查询词典 fibroblasts

Copper levels in skin fibroblasts of the involved vitiliginous skin were much lower than those of the uninvolved, but slighty lower than or the same with those of controls.

近年来发现白瘫风患者的血清铜和铜蓝蛋白含量降低囚，作者对白瘫风患者的皮损区、周围区的皮肤成纤维细胞进行了细胞培养和铜含量测定。

A complete bovine 18ku-bFGF gene was cloned by nested-PCR and subcloned into expression vector pET-28a.The recombinant plasmid pET-28a-bFGF was transformed into Escherichia coli BL21.At 25℃,recombinant protein was induced by 0.5mmol/L IPTG for 5 hours.Supernatant of cell lysate was purified using Ni-NTA method and the products was submitted to detect the bioactivity by Western-blotting,which indicated that the fusion protein contained recombinant bovine bFGF.Results showed that recombinant bovine bFGF was produced and solubly existed in the supernatant.NIH-3T3 fibroblasts were used to detect the bioactivity of the fusion protein.

采用巢式PCR方法克隆了牛18ku-bFGF基因完整的编码序列，并构建了原核表达载体pET-28a-bFGF，将其转化大肠杆菌BL21，在25℃低温条件下，用0.5mmol/L IPTG诱导表达5h，用Ni-NTA亲和纯化细胞裂解上清液，经Western-blotting检测，结果显示，在特定的诱导条件下，重组牛bFGF基因在大肠杆菌中获得了表达，并且主要以可溶性状态存在于细胞中。

ICR fetus mice were sterilely obtained to culture mouse embryonic fibroblasts by tissue pancreatin digestion method.

无菌条件下取出ICR胎鼠，组织块胰酶消化法分离培养小鼠胚胎成纤维细胞，接种在0.1%明胶包被的6孔板中即为饲养层。

Objective To investigate the phenotypic change and proliferation of fibroblasts in human inflammatory strictured bile duct wall.

目的 探讨炎性狭窄胆管壁中成纤维细胞表型改变及增殖在炎性胆管狭窄发生中的作用。

Methods We observed the density and ultrastructure of fibroblasts, and the histologic structure in human normal bile duct wall and inflammatory strictured bile duct wall by light and electron microscope.

对人正常胆管壁及炎性狭窄胆管壁组织学结构及其成纤维细胞的密度和超微结构进行观察。

Objective: To explore the mechanisms resulting in the recurrence of urethral scar which make urethral strictures difficult to be cured, a series experiments were conducted to find potential effective factors involved in urethral scar formation and degradation, including the studies of extracellular matrix component of urethral stricture scar, the characteristics of urethral scar fibroblast, and the effects of urine on urethral fibroblast in vitro, as well as the studies to compare the difference of collagenase activity, type Ⅰ collagen and tissue inhibitor of metalloproteinase-1 (TIMP-1) in the tissues and cultured fibroblasts from normal urethra and strictured urethra respectively, and the studies to investigate the effect of antisense TIMP-1 oligodeoxyonucleotide on cell proliferation and collagenase activity of urethral scar fibroblast.

中文题名尿道瘢痕基础研究副题名胶原酶活性，TIMP-1的表达及其反义基因治疗外文题名 Experimental study on urethral scar-activity of collagenase，expression of TIMP-1，and antisense TIMP-1 gene transfection of urethral scar fibroblast 论文作者黄翔导师杨宇如教授学科专业外科学研究领域\研究方向学位级别博士学位授予单位四川大学学位授予日期2002 论文页码总数104页关键词尿道手术瘢痕胶原酶成纤维细胞尿道瘢痕馆藏号BSLW /2003 /R699 /12 目的：研究尿道瘢痕的细胞外基质的组成，尿道瘢痕成纤维细胞的生物学特性以及尿液对其生长的影响；比较胶原酶活性，金属蛋白酶组织抑制因子-1（TIMP-1）以及Ⅰ型胶原含量在尿道瘢痕和正常尿道组织及体外培养的成纤维细胞中的差异；研究反义TIMP-1寡核苷酸对尿道瘢痕成纤维细胞增殖以及胶原酶活性的影响。

IH is characterized by the invasion and proliferation of vascular smooth muscle cells and fibroblasts, causing hyperplasia in the subendothelial plane.

内膜增生一般是由平滑肌细胞、成纤维细胞的增生并向新生内膜侵润而引起。

Hideyuki Doi, Nakui Madaki, Hrroshi, Hiromichi Komatsu, Keisei Fujimori and Susumu Satomi Tohoku. A New Preadipocyte Cell Line,AP-18,Established from Adult Mouse Adipose Tissue. J.Exp.Med,2005,207:209-216[2] Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblasts by PPARγ 2, a lipid-activated transcription factor. Cell ,1994,79:1147－1156

PPARα调节脂肪代谢的机制总结如下：肝脏脂肪酸水平的增高激活PPARα，然后转录激活参与脂肪酸氧化的基因；配体与之结合后可激活编码乙酰辅酶A合成酶的基因，同时PPARα激活后可调节脂肪转运相关基因的表达如PPARα升高高密度脂蛋白和抑制ApoC－Ⅲ的表达。

HE staining revealed that collagen fiber, fibroblasts and granulation tissue and synechia belt around tendons and stomas were apparently less in contrast to control group.

术后4周苏木精－伊红染色结果表明，实验组腱吻合口被胶原纤维连接，腱周及吻合口周围胶原纤维、成纤维细胞及肉芽组织明显较对照组少，腱周纤维组织较对照组粘连轻，粘连带稀少。

There were synergistical effect on proliferation, but no synergistical effect on differentiation in periodontal ligament fibroblasts on groups of rhBMP-4 plus rhIGF-Ⅰ.

rhBMP-4与rhIGF-Ⅰ联合应用，对促进人牙周膜成纤维细胞生长增殖有协同作用，但对细胞分化并无协同作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐