查询词典 fibroblasts

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

AIM: To evaluate the effect of retinoic acid on chick scleral fibroblasts proliferation and ultrastructural changes in vitro.

摘要目的：研究视黄酸(retinoic acid，RA)对体外培养的鸡巩膜成纤维细胞的生长及其超微结构的影响。

RA inhibits chick scleral fibroblasts proliferation significantly in vitro and causes significant ultrastructural changes.

RA对体外培养的鸡巩膜成纤维细胞的增殖有明显的抑制作用，并引起其超微结构的改变。

The effect of RA on chick scleral fibroblasts ultrastructure was observed by transmission electron microscope.

另分别取对照组及6×10-5 mol/L RA组对数生长期2代细胞铺片采用透射电镜观察其超微结构的改变。

Objective To study H2O2-induced single strand breaks formation in DNA and the reparation In NIH3T3 mouse fibroblasts by single cell gel electrophoresis technique and to introduce SCOB.

目的 观察小鼠成纤维细胞由过氧化氢(H2O2)引起的DNA损伤及其修复，并详细介绍单细胞凝胶电泳技术。

In vitro cytotoxicity experiments the IC50 value for HeLa,K562 and MCF-7 tumor cells is 0.6 mol/L, 0.8 mol/L and 4 mol/L separatedly, while for the normal human fibroblasts cells, even up to 8 mol/L, no cytotoxic effects were observed.

在体外细胞毒性实验中，对人三种肿瘤细胞HeLa、K562、MCF-7具有抑制作用，其IC50分别为0.6 mol/L、0.8 mol/L和4 mol/L，而对于正常人成纤维细胞在酶浓度达到8 mol/L时仍未见明显细胞毒性。

Pulmonary arterial fibroblasts from SERT mice, but not wild-type mice, proliferated in response to hypoxia.

SERT组小鼠的肺血管成纤维细胞因慢性缺氧发生增值，而野生型小鼠没有出现增值。

The cells were shammed or irradiated with different dosages of UVB radiation and treated with the above TCM monomers. The damage of fibroblasts was observed with inverted phase contrast microscope.Cellular proliferation activity was detected by MTT method.The secretory volume of IL-6 and TNF-α was measured by ELISE. Results The damage degree of the cells irradiated was dependent with the irradiated doses and the proliferation activity reduced by 28%-44%.

采用30mJ/cm^2、60mJ/cm^2、90mJ/cm^2剂量UVB照射体外培养的原代人皮肤成纤维细胞，以中药单体EGCG和黄芩苷进行干预处理，倒置相差显微镜观察细胞受损程度，以甲唑盐比色试验检测细胞增殖活性，酶联免疫吸附试验检测上清液TNF-α、IL-6分泌量。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐