查询词典 fibroblasts

aim: to study the effect of urokinase on the cell proliferation and apoptosis of rat pleural fibroblasts.

目的：研究尿激酶对大鼠胸膜成纤维细胞增殖与细胞凋亡的影响。

In normal postauricular skin, EGF was expressed in squamous epithelium in 14 samples (41%), fibroblasts in one sample (3%), and endothelium in none. No statistical difference in EGF expression was found between cholesteatoma and normal postauricular skin samples.

在中耳胆脂瘤组织细胞中，EGF阳性百分比在squamous epithelium、endothelium及fibroblast细胞分别为53%（21例）、5%（2例）及5%（2例）；而在耳廓后皮肤组织中，其阳性率分别为41%（14例）、0%（0例）及3%（1例）。

The purpose of this study is to evaluate the proliferative activity of fibroblasts in lung cancer, and to explore its correlation with tumor biologic behavior.

背景与目的肿瘤细胞与间质细胞的相互作用在肿瘤的侵袭和转移中起着重要作用。

Results The hEFs were successfully isolated and cultivated from gonadal ridges and dorsal mesenteries of human embryos. They could be passaged beyond the 25th generation. The biologic characteristics of the cells did not change, even in high-passage cells or frozen-thawed cells. The cells expressed prolyl 4-hydroxylase β, but not cytokeratin-4, which was similar to the fibroblasts. The cultured cells expressed bFGF and LIF.

结果：从人胚胎生殖腺嵴和肠背系膜中成功地分离培养出hEFs，该细胞可传25代以上，且经过传代及冻存复苏后生物学特性无改变；hEFs表达脯氨酰4-羟化酶β亚单位，不表达细胞角蛋白4，确定其为成纤维细胞；该成纤维细胞表达bFGF和LIF。

In this study, the genomic structure and regulatory elements of ApoD from 10 representive species including protozoan, invertebrate, protochordate and vertebrate were analyzed and compared. The genomic structure of ApoD is less conserved in organisms from protostome to deuterostome invertebrates, while it is highly conserved among chordates including amphioxus and verebrates. All four conserved cysteine residues are present in amino acid sequence of deuterostome ApoDs, while there are only two cysteine residues in amino acid sequence of protostomes ApoD. Structure divergence between protostome and deuterostome ApoD proteins suggests their function difference. The majority of regulatory elements are present in nearly all organism ApoD genes ranging from unicellular protozoan to mammals, suggesting that ApoD plays a very fundamental role, and possesses a conserved regulatory mechanism. However, there also exist some specific regulatory elements, which are present only in certain species and may perform some special roles.ApoD mRNA expression in murine NIH/3T3 fibroblasts exposed to various stresses such as as hydrogen peroxide and UV light shows dose-dependent increase. And a fly homolog of ApoD, Glial Lazarillo, whose overexpression results in increased resistance to hyperoxia as well as a extension of lifespan under normoxia and resistance to starvation without altering lipid or protein content.

本文首先从生物信息学角度对分属于原生动物、无脊椎动物、头索动物和脊椎动物类群的10种动物ApoD的基因结构及调控区的调控元件进行分析及比较，发现：(1)ApoD基因外显子-内含子结构从原生动物草履虫到原口动物再到后口动物海胆的进化过程中不保守，但在分析的几种脊椎动物中相当保守；(2)文昌鱼ApoD基因扮演从无脊椎动物到脊椎动物承上启下的角色，可能代表了脊椎动物ApoD基因原型；(3)四个半胱氨酸保守位点在后口动物中都存在，而在原口动物中只存在两个，原口、后口动物ApoD蛋白一级结构上的差异反映蛋白功能上可能也存在一定差别；(4)调控区大多数主要调控元件为不同动物共有，说明ApoD主要功能及其表达调控在进化中相当保守；(5)ApoD基因个别调控元件是随着物种进化而出现并开始发挥相关作用，如SF-1；还有一些调控元件在进化过程中还没有发现其规律，这说明ApoD某些功能和基因表达调控模式可能因物种不同而存在一定的差异。

To observe the effect of diterpenoid compound 5F from Pteris semipinnata L.(5F) on the cell proliferation, collagen synthesis and the expression of connective tissues growth factor in human pterygium fibroblasts cultured in vitro.

观察半边旗二萜类化合物5F(5F)对体外培养的人翼状胬肉成纤维细胞增殖和胶原合成及结缔组织生长因子表达的影响。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

收集翼状胬肉标本，采用血管内皮细胞和成纤维细胞单独培养、条件培养和共同培养的方法构建体系，用MTT法选择紫外线照射细胞的适宜强度；采用MTT法绘制强度20mJ/平方公分紫外线照射下细胞生长曲线；采用ELISA和RT-PCR检测紫外线照射下3种体系中细胞上清液和细胞中血管内皮细胞生长因子和成纤维细胞生长因子的蛋白和RNA含量变化。

Objective:To observe the biological effect of bFGF on fibroblasts from peridongal ligament,and evaluate the value of bFGF in clinical pulpal and periodontal therapy.

R781.4A1005-2720-（2010）03-0003-02目的：了解bFGF对人牙周膜成纤维细胞的生物学效应，为bFGF在牙周病中的治疗学研究提供新的实验依据。

The COX-2 positive cells were odontoblasts, pulpal fibroblasts vascularendothelial cells and macrophages (positive neutrophils could be found in some cases of the second group and of the third group).(3)We found that COX-2 developed up with HIF-1 a in third group ,but against it infourth group.

COX-2表达阳性的细胞有成牙本质细胞、成纤维细胞、血管内皮细胞和单核巨噬细胞(阳性中性粒细胞可出现在第二组和第三组)，HIF-1α与COX-2的表达相关主要在第三组和第四组牙髓标本中体现明显，在第三组二者的变化大致呈平行关系；在第四组基本呈反向变化关系。

AIM: Investigation of bio-effects of GM- CSF on periodontal ligament and pupal fibroblasts.

目的：观察GM-CSF对牙周膜成纤维细胞、牙髓成纤维细胞的生物学作用。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐