查询词典 fibroblasts

Objective: To study the expression of integrins in fibroblasts in patients with scleroderma.

目的：研究硬皮病皮损成纤维细胞整合素表达及细胞超微结构特征。

Objective To observe the effects of IL-1α derived from keratinocytes on proliferation and collagen secretion of fibroblasts.

目的：研究角质形成细胞分泌的IL-1α对成纤维细胞生物学行为的影响。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

In order to study the protection of β-carotene and vitamin C against the transformation of human lung fibroblasts cell induced by nickel sesquioxide, HLF cells were treated repeatedly by different concentrations of Ni2O3 in vitro.β-carotene (5.4mg/L) and vitamin C(1.8mg/L) were added into the media containing Ni2O3 (1.0mg/L) respectively. The identification of malignancy of the transformation of HLF cell was carried out by the tests of ConA and the growth on semisolid agar culture. The results showed that Ni2O3 could induce the malignant transformation of HLF cell. The transformed cell proliferated rapidly.

为了探讨三氧化二镍(Ni2O3)诱导人肺成纤维细胞恶性转化作用以及β-胡萝卜素和维生素C的保护作用，用不同浓度的Ni2O3在体外多次处理HLF细胞，利用刀豆凝集素A凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定；同时添加β-胡萝卜素(5.4mg/L)和维生素C的食物(1.8mg/L)，观察对Ni2O3转化作用的影响。

AIM: To study the expression of MMP2 and MMP9 in fibroblasts of rat silicotic fibrosis model and its significance.

目的：探讨肺间质成纤维细胞MMP2和MMP9在SiO2致肺纤维化中的表达及意义。

In fibroblasts of rat silicotic fibrosis model and its significance.

2和MMP？？？？9在SiO2致肺纤维化中的表达及意义。

JC| o tl9^4M AIM: To study the expression of MMP2 and MMP9 in fibroblasts of rat silicotic fibrosis model and its significance.

O8R-gr1STXj，Z 目的：探讨肺间质成纤维细胞MMP2和MMP9在SiO2致肺纤维化中的表达及意义。

To explore the potential mechanism of cigarette smoke extract -induced cell injury, primary cultured skin keratinocytes and fibroblasts from rat were selected as target cells in Part Ⅰ study compared with the effect of UVA.

本研究第一部分以原代培养的大鼠皮肤角质形成细胞和成纤维细胞为对象，采用UVA损伤作用为参照，探索香烟雾提取物（Cigarette SmokeExtract，CSE）对皮肤细胞的损伤作用，重点观察CSE所致的皮肤细胞毒作用、功能损伤及可能作用机制。

Results With different concentration of sodium arsenite 0.5 ～ 5.0 μmol/L, the skin fibroblasts were markedly stimulated, and a dose effect relationship was observed.

结果与对照组比较，皮肤成纤维细胞经不同剂量的亚砷酸钠诱导后，吸光度值均有改变，存在剂量效应关系（P〈0.01）。0.5～5.0μmol／L浓度的亚砷酸钠对皮肤成纤维细胞有明显的增殖作用，而10μmol／L浓度的亚砷酸钠产生明显的毒性作用。

Methods Cell number counting, MTT reduction assay, transmission electron microscope were performed in skin fibroblasts,and sodium arsenite was used as tester for inorganic arsenics.

方法通过直接细胞计数法和噻唑蓝还原法检测亚砷酸钠对皮肤成纤维细胞生长的影响。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。