查询词典 fibroblast

MAIN OUTCOME MEASURES: Preparation of fibroblast feeder layer; growth of embryonic stem cells; results of alkaline phosphatase staining; cell differentiation tendency.

主要观察指标：成纤维细胞饲养层的制备情况，胚胎干细胞的生长状态、碱性磷酸酶染色结果及分化趋势。

Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中，我们在相同条件下，将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells，HEL)和3株传代细胞株：人肝癌细胞(Human hepatic carcinoma 3B cells，Hep-3B)；人肺腺癌细胞(Human lung carcinoma A549 cells，A549)；鼠正常成纤维细胞(Normal mouse fibroblastscells，NIH 3T3)。

Objective To study the antitoxic effect of basic fibroblast growth factor against guinea pig kidney injury induced by gentamicin.

目的 研究碱性成纤维细胞生长因子对庆大霉素所致豚鼠肾损伤的拮抗作用。

Objective To study the role of fibroblast in aseptic loosening.

目的 探讨成纤维细胞在无菌性松动中的作用。

OBJECTIVE To explore the effect of basic fibroblast growth factor combined with autogenous vein graft conduit on peripheral nerve regeneration.

目的 探讨碱性成纤维细胞生长因子与静脉套接法修复神经缺损的作用。

Acellular allogeneic dermal matrix is the dermal replacement of heterogenous human skin in which the cellular elements have been removed by special technique and basement membrane complex have been remained. It becomes a kind of 3D porous acellular dermal scaffold. It supports fibroblast infiltration, neovascularization and epithelialization in the absence of an inflammatory response.

异体脱细胞真皮基质（acellular allogeneic dermal matrix， ADM）来源于正常的异体皮肤组织，经过特殊的理化处理去除真皮中的细胞成分，保留原有的胶原三维结构和完整的基底膜，成为一种多孔的三维膜性支架，其空间网状结构有利于细胞的增殖、毛细血管增生，加速组织的愈合过程，移植后可以迅速被血管化和自体细胞重建。

Objective:The aim of this study is to determine the content of vascular endothelial growth factor and basic fibroblast growth factor in the wound fluid during maxillofacial soft tissue blast injury, and to evaluate their effects on angiogenesis of wound healing.Methods: The rabbit model of maxillofacial blast injury was made by KTY_04 blasting cap.

目的：探讨颌面部软组织爆炸伤愈合过程中血管内皮细胞生长因子和碱性成纤维细胞生长因子的分布和在伤口液中含量的动态变化，及其对血管再生的刺激作用。

RESULTS: In the verapamil group, the fibroblast were round or oval, and there were tiny cell process, less alpha substance, atrophic and decreased rough endoplasmic reticulum in kytoplasm and abundant vesica. In control group, there were fusiform or polygon fibroblast, and much matrix among cells, abundant rough endoplasmic reticulum and ribosome in cytoplasm and less vesica were found.

结果：维拉帕米组神经瘢痕成纤维细胞呈球形或椭圆形，细胞突起细小，胞间网状物质少，胞浆中粗面内质网萎缩且数量减少，囊泡丰富；对照组成纤维细胞呈梭形或多角形，细胞间基质较多，细胞浆内粗面内质网及核糖体丰富，囊泡少见。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

Studies on mouse nuclear transfer with adult male mouse ear fibroblast cells as donors In this study, C57BL/6 adult male mouse ear fibroblast cells and Kunming mouse MⅡoocytes were used as donors and recipients, respectively, to investigate the effects of different passages of donor cells and electrofusion times on the in vitro development of NT reconstructed embryos, when the oocytes were treated with 3% sucrose.

以成年雄鼠耳成纤维细胞为供体克隆小鼠的研究该研究以C57BL/6 成年雄鼠耳成纤维细胞为供体，昆明白小鼠MⅡ期卵母细胞为受体，研究了3%蔗糖处理卵母细胞去核后，供体细胞不同传代次数和电融合次数对核移植重构胚体外发育的影响。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐