查询词典 fibroblast

A strain of new virus was isolated from fledgling budgerigars by authors in 1995. Cytopathic effect became visible after the isolate was passaged 4 generations on budgerigar embryo fibroblast and 5 generations on Chicken embryo fibroblast.

摘 要 1995年作者从湖北濒死鹦鹉体内分离到一株新病毒，经鹦鹉胚成纤维细胞盲传四代过渡到鸡胚成纤维细胞，再传至第五代出现细胞病变。

In test group fibroblast growth factor immunoreactivity was detected in 89% of the samples, mRNA was detected in 20% of the samples. In control group no fibroblast growth factor immunoreactivity and mRNA was detected.

结果：实验组30例免疫组化和原位杂交的阳性率分别为89％和20％，对照组6例免疫组化和原位杂交均为阴性，两组免疫组化方法检测阳性率有显著差异。

The cells were passaged no more than 5 generations,and the cell freezing density is about 3×106 cell/ml, sample numbers of each kind are between 30 and 60, conserved numbers are 150-180 ampules,biological characters of fibroblast were analysed according to the request of constructing cell line. The results were as follows: 1 Cultured cells'morpha is typical fibroblast,which is fusiformate or unregular triangle, cells is radiant,flaming or rotative during growing .Qingyuan flesh chicken's cytoplasmic tubercle is much more and shorter in contrast with Zang chicken and Langshan chicken,that can be regarded as different character from others.

细胞冻存代数在5 代以内，冻存密度为3×106个细胞/ml，每个品种的样本含量在30～60 个之间，保存管数达150～180 支，根据建系要求，对成纤维细胞的生物学性状进行分析，所得结果如下： 1 培养细胞形态为典型的成纤维状，为梭形或不规则三角形，细胞在生长时呈放射状、火焰状或旋涡状走形，清远麻鸡的细胞形态与藏鸡和狼山鸡相比，胞质向外伸出的突起较多，且较短。

An attenuated vaccine strain of Muscovy Duck Reovirus, which was suitable for propagating in chicken embryo fibroblast, was obtained by serial passage alternately between Muscovy duck embryo fibroblast and CEF.

应用番鸭胚成纤维细胞和鸡胚成纤维细胞交替传代选育出适应CEF繁殖的番鸭呼肠孤病毒弱毒疫苗株。

In this system, tubular scaffold can rotate slowly around own major axis. By means of this, three-dimension tissuesor organs can be cultured in vitro. Part IIIHVEC, VSMC and fibroblast were seeded onto inner surface of tubular porous PGA and PHB scaffold precoated with collagen. Sead HVEC,VSMC and fibroblast onto three porous polyglycolic acid and Poly-?

我们还特别设计了旋转培养装置，通过微型马达驱动，减速装置作用使旋转部分绕自身中心水平轴做缓慢转动，从而使置于旋转部件上的管形支架绕其正中长轴作缓慢旋转，其原理与美国 Genzrpe公司的 RCCS（Rotary cell culture system RCCS）系统相仿。

Then the caprine fetal fibroblast cells were transfected with p6.5hLF-EGFP by LipofectamineTM-2000 and selected by G418 for 3 to 4 weeks. The G418 resistant transfectants were identified by PCR and EGFP detection. The results indicated that the transgene was stably integrated into the open region of the chromatin of G418 resistant fibroblast cells.

脂质体介导法转染山羊胎儿成纤维细胞，G418抗性筛选3-4周后，经PCR扩增和报告基因EGFP表达检测，得到稳定整合外源基因的转基因供体细胞系，为制备高效表达人乳铁蛋白的转基因山羊乳腺生物反应器提高可靠的核移植供体细胞。

Majority of aderent cells from BM were fibroblast-like cells, and small parts were endothelioid cells. Aderent cells from PB and CB at the fifth generation contained more endothelioid cells and mononuclear and macrophage-like cells besides fibroblast-like cells.

初始培养和传代培养后，骨髓间充质干细胞多为成纤维样细胞，仅有少量内皮样细胞；而传至第5代的外周血、脐带血间充质干细胞除有成纤维样细胞外，还有较多的内皮样细胞和单核－巨噬样细胞。

Immunofluorescence cytochemistry was used to detect the expression of IPSCs fibroblast growth factor receptor 3, the expressions of SV40Tag and fibroblast growth factor receptor 3 in the human precartilaginous stem cells were determined by RT-PCR.

以免疫荧光细胞化学技术检测永生化人前软骨干细胞成纤维生长因子受体3，以RT-PCR检测永生化人前软骨干细胞及人前软骨干细胞中SV40Tag和成纤维生长因子受体3表达。

Objective: Mast cell tryptase has been implicated in regulation of skin fibroblast, lung fibroblast and hepatic stellate cell proliferation and collagen synthesis.

目的：肥大细胞类胰蛋白酶已被证明在皮肤、肺成纤维细胞和肝星型细胞增生和胶原合成中具有重要作用。

The histopathologic changes included lymphocyte and monocyte infiltration,hyperplasia of synovial cells and small vessels,interstitial fibrosis,hyaline degeneration and cartilaginous metaplasia.The immunohistochemical observations showed that the high expressions of IL-1 and IL-6 were significantly different between the pathologic plicae and the control groups（P＜0.01）.The positive expressions of IL-1 and IL-6 were the synovial cells and monocyt-lymph cells in the pathologic synovial plicae.The positive expressions of MMP-1 and TIMP-1 have significant difference between the experiment and control group（P＜0.01,P＜0.05）.The expression of MMP-1 was positive in synovial lining cell,monocyte,fibroblast,endothelial cell in small vessel and chondrocyte.The TIMP-1 expression was detected in the synovial lining cells and a small quantity fibroblast.

结果 正常滑膜皱襞和病理性滑膜皱襞在滑膜细胞增生及小血管增生、间质纤维化及玻璃样变、软骨化生组织学改变方面，差异均有显著性（P＜0.01）；IL-1、IL-6在病理性滑膜皱襞内的增生滑膜细胞、单核及淋巴细胞和在正常滑膜皱襞内的表达差异均有显著性（P＜0.01）； MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达，差异具有显著性（P＜0.01，P＜0.05），MMP-1在增生滑膜衬里层细胞、单核和纤维母细胞、血管内皮细胞和软骨化生的软骨细胞呈阳性表达；TIMP-1只在滑膜衬里层细胞和少量纤维母细胞有表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐