查询词典 fibroblast

Expanded MSCs were induced to differentiate into nerve cells withthree different treatment protocol in the prensent study . On the following day, the medium was replaced with preinduction medium consisting of DMEM, 20% FCS, and 10 ng/ml basic fibroblast growth factor. After 24 h, the preinduction medium was removed, the cells were washed twice with PBS, and neuronal induction medium containing DMEM supplemented with 2% DMSO and200 m bu-tylated hydroxyanisole was added. In later experiments, we used DMEM with 5 mM p - mercaptoethanol or sulfo - glycerine as an alternative neuronal induction medium for the same incubation.

将待诱导分化的MSCs按0.4x10~6／ml接种于事先放置有消毒盖玻片的六孔板内制备细胞爬片，分别采用三种不同的方法诱导第2代至10代的MSCs向神经细胞分化，即方法1先用含bFGF的培养基进行预诱导，之后再用含叔丁基对羟基茴香醚，和二甲亚砜的无血清培养基进行诱导；方法2和方法3用B-巯基乙醇和硫代甘油进行预诱导，然后不同浓度的上述物质进行正式诱导。

The goat fetal fibroblast cells were transfected by linear GBCt-PAm with electroporation or lipidosome, and after the identification of PCR, we got 7 lines of progenitive cells that examined positive.The two cell line (M9, M10) had been used for the somatic nuclear transfer for the first time.

将pGBCt-PAm去除原核序列后回收线性片段，采用电击和脂质体转染两种方式转染山羊胎儿成纤维细胞，经G418筛选培养基筛选及PCR检测，最终得到了7株能有效扩增的PCR检测阳性的细胞克隆。

To observe the influence of hirudin on the cultured sclera flbroblast and extracellular matrix, and research the mechanism of hirudin treating the diseases caused by the proliferous fibroblast.

实验目的通过观察水蛭素对体外培养的巩膜成纤维细胞及细胞外基质的影响，从而探讨水蛭素防治由成纤维细胞增生引起的疾病的机制。

The reverse transcription-polymerase chain reaction was used to seek the expressions of a specific fibroblast marker, prolyl 4-hydroxylase β, and a specific marker of epithelial cells, cytokeratin-4, in the cells.

采用逆转录多聚酶链反应检测hEFs表达成纤维细胞特异性标志（脯氨酰4-羟化酶β亚单位）和上皮细胞特异性标志（细胞角蛋白4）的情况，对该细胞进行鉴定。

Objective To characterize the mechanism of promotive effects of basic fibroblast growth factor on proliferation of lens epithelial cells.

目的探讨碱性成纤维细胞生长因子（basicfibroblastgrowthfactor，bFGF）对晶体上皮细胞促增殖作用的机理。

Objective: To investigate expression level of b-FGF and probe into the function of basic-Fibroblast Growth Factors in congenital pseudarthrosis of tibia.

目的：明确碱性成纤维细胞生长因子在先天性胫骨假关节骨膜中的表达水平，探讨b-FGF在CPT发病中的作用。

In SW620 cell line, there were more fibroblast-like cells with many microvillus and pseudopodia on the cell surface and loose inter-cells conjunction.

由于 不同转移特性癌细胞存在膜表面分子种类和数量等的差异，其超微结构的形貌也必然存在或多或少的差异。

In SW620 cell line, there were more fibroblast-like cells with many microvillus and pseudopodia on the cell surface and loose inter-cells conjunction.

SW620瘤细胞纤维母细胞样细胞较多，表面有较多的微细绒毛和细长胞突，瘤细胞间黏附不紧密。

Methods Pterygial samples were extracted and collected and the pterygial endothelial cells and pterygial fibroblasts were cultured alone, conditional and co-cultured to form different culture systems. The methods included that to select the suitable intensity of ultraviolet by MIT, to detect the changes of curves of growth about two kinds of cells by MIT and to explore the developments of protein and RNA of vascular endothelial growth factor and fibroblast growth factor-basic in three culture systems under ultraviolet whose intensity is 20 mJ/cm^2 by ELISA and RT-PCR.

收集翼状胬肉标本，采用血管内皮细胞和成纤维细胞单独培养、条件培养和共同培养的方法构建体系，用MTT法选择紫外线照射细胞的适宜强度；采用MTT法绘制强度20mJ/平方公分紫外线照射下细胞生长曲线；采用ELISA和RT-PCR检测紫外线照射下3种体系中细胞上清液和细胞中血管内皮细胞生长因子和成纤维细胞生长因子的蛋白和RNA含量变化。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐