查询词典 fibroblast

Methods: Immunohistochemical study of CD133 expression: 55 cases osteosarcoma conventional paraffin-embedded tissues specimens come from Pathology Department Qilu Hospital Shandong University, including 4 cases parosteal osteosarcoma, 13 cases fibroblast-type osteosarcoma , 12 cases chondroblastic osteosarcoma and 26 cases Osteoblastoma osteosarcoma.

免疫组化研究CD133的表达：取自本院病理科的骨肉瘤常规石蜡包埋组织蜡块标本55例，其中骨旁骨肉瘤4例，纤维母细胞型骨肉瘤13例，软骨母细胞型骨肉瘤12例，骨母细胞型骨肉瘤26例，切成3μm的组织切片备用。

AIM: To construct,overexpress and purify a stable and active form of shortened human acidic fibroblast growth factor in Escherichia coli.

目的：稳定且具有活性的截断型人酸性成纤维细胞生长因子的构建、表达及纯化。

MethodsThe transplanted tumor model of the mouse S180 sarcoma was established.Fifty mice were randomly divided into five groups,the control group,the CTX group,the Gecko group.They were treated respectively with oral administration of saline,and intraperitoneal injection of CTX 100 mg/kg only one time,oral administration of Gecko in doses of 13.5,9,4.5 g/kg,one time a day.After 14 days,the anti-tumor activity was evaluated by tumor tissue weighing. The impact on immune organ was detected by accounting thymus index, spleen index and the number of peritoneal macrophage which phagocytose chicken red blood cells.The protein express of vascular endothelin growth factor and basic fibroblast growth factor were detected by SABC immunohistochemistry.

方法建立移植瘤小鼠S180肉瘤模型，将50只雌鼠随机分为对照组、环磷酰胺组、壁虎高组、中组、低组共5组，分别给于生理盐水灌胃1次/d，CTX（100 mg/kg）腹腔注射1次，壁虎高、中、低组（13.5，9，4.5 g/kg），每天灌胃1次。14 d后，称取荷瘤小鼠瘤重、胸腺重、脾脏重，计算抑瘤率、胸腺指数、脾脏指数，观察腹腔巨噬细胞吞噬鸡血红细胞实验指标；用SABC免疫组化法检测血管内皮生长因子、碱性成纤维细胞生长因子的蛋白表达，TUNEL方法检测细胞凋亡率。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色：翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成；基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质，在翼状胬肉体部深层基质中存在粗糙的纤维组织；正常球结膜组织细胞排列整齐；基质为疏松结缔组织，胶原纤维平行排列，其间可见成纤维细胞，散在少量中性粒细胞、毛细血管；Masson染色：翼状胬肉浅层基质中存在致密的胶原纤维染色，深层基质中的胶原纤维存在变性样改变；VG染色：翼状胬肉组织深层基质中存在大量变性的胶原纤维，其间夹杂黑色的弹性纤维；免疫组化染色法：MMP-3在翼状胬肉上皮细胞中呈强表达，成纤维细胞中呈中等强度表达，血管内皮细胞中未见表达；LN在血管壁中呈强表达，在上皮细胞基底膜中呈中等强度表达，在整个基质中未见明显表达；col Ⅲ在整个翼状胬肉基质中呈强表达。

The therapeutic mechanism of the method of nourishing qi, activating blood, dispersing phlegm and promoting the collaterals was supposed as that it intervened the airway remodeling and alleviated the progress of airflow obstruction of COPD by suppressing the chemo taxis of cytokines, regulating the proliferation of fibroblast cell and smooth muscle cell and preventing the proliferation and plerosis of extracellular matrix.

推测益气活血化痰通络法防治COPD的机制可能为：通过抑制炎症细胞的趋化、影响细胞因子的活性、调节成纤维细胞和平滑肌细胞的增殖、阻止细胞外基质的增生修复，从而达到干预COPD患者气道结构重塑，减缓COPD气流受限进展的作用。

We have found that plumula nelumbinis, euphorbiae fischerianae and euphorbiae can inhibit the proliferation of rat lung fibroblast. Plumula nelumbinis is the best drug with most effective and little cytotoxic action.

I热川川川川川川~蔗选，寻找到了莲子心、狼毒、千金子、这三种对大鼠肺成纤维细胞有抑制作用的中草药，其中效果最佳、细胞毒作用最小的为莲子心提取物。

The mechanism of action maybe include the depression in content of collagen, inhibition to proliferation of lung fibroblast, action of anti free redical and anti lipid peroxidation, and elevation of content of GSH-PX.So we think phenol total base of plumula nelumbinis is a sort of promising drug to healing PF.

综上所述，我们认为莲子心酚性总碱可能对肺纤维化的病理过程有多靶点作用：前期通过抗自由基作用，从而减轻肺泡炎损伤：后期直接抑制肺成纤维细胞的增殖及蛋白沉积，减轻纤维化病变程度。

In normal postauricular skin, EGF was expressed in squamous epithelium in 14 samples (41%), fibroblasts in one sample (3%), and endothelium in none. No statistical difference in EGF expression was found between cholesteatoma and normal postauricular skin samples.

在中耳胆脂瘤组织细胞中，EGF阳性百分比在squamous epithelium、endothelium及fibroblast细胞分别为53%（21例）、5%（2例）及5%（2例）；而在耳廓后皮肤组织中，其阳性率分别为41%（14例）、0%（0例）及3%（1例）。

It can prevent growth of fibroblast and formation of fibrin bundles. So it can prevent postsurgical adhesions effectively.

本品可用于多种手术中，防止或减少术后组织粘连的形成，如妇产科手术、普通外科手术、骨科手术、神经外科手术、肿瘤手术、各种腹腔镜手术等。

Preinduction was performed using basic fibroblast growth factor, which could promote the differentiation from BMSCs into nerve precursor cells or neurons. The expression of neuroprotein molecule was coincided with the development of neural cells.

在添加化学诱导剂之前先使用成纤维生长因子进行预诱导，能促进骨髓间充质干细胞向神经前体细胞和神经元转化，且在诱导分化过程中神经蛋白分子的表达顺序与神经细胞发育过程一致。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐