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Abnormal chromosome was detected in 17 fetuses. Among them, trisome 21, trisome 18, trisome 13, Turner syndrome, Klinefelter syndrome, short arm deletion of chromosome 18, and inverted chromosome 9 were detected in 8, 3, 1, 1, 1, and 2 fetuses, respectively. Abnormal chromosome was found in 5 out of 8 fetuses who received serum screening. Ultrasound showed abnormal chromosome in 11 fetuses.

共诊断17例胎儿染色体异常,其中21三体8例,18三体3例,13三体1例, 1 Turner综合征1例,Klinefelter综合征1例, 18染色体短臂微缺失1例,9号染色体臂间倒位2例(1例来源于母亲)。17例中有8例进行了唐氏儿血清学筛查,5例异常,11例超声检查胎儿有结构异常。

Methods Fetal echocardiographic examinations were performed in 144 pregnant women, included 70 fetuses with nuchal cord and 74 fetuses without nuchal cord and fetal distress.

144例行胎儿彩色多普勒超声检查的孕妇,其中脐带绕颈者70例,对照组为74例无脐带绕颈及无宫内窘迫者,监测两组胎儿的左、右心室Tei指数。

Five fetuses were diagnosed with female pseudohermaphroditism (21-hydroxylase deficiency in 3 and urorectal septum malformation sequence in 2). Four fetuses were diagnosed with male pseudohermaphroditism (1 with steroid sulfatase deficiency, 1 with presumed camptomelic dysplasia, and 2 undetermined).

在这16个胎儿中有5个被诊断为女性假两性畸形(其中3个有21-羟化酶分泌不足,2个有尿道直肠隔畸形),4个被诊断为男性假两性畸形(其中1个有类固醇硫酸酯酶分泌不足,1个推测可能为弯肢性发育异常,另外2个原因未定),5个有染色体异常,2个有性逆转,核型为(46,XX+SRY)。

The nasal bone was absent in 43 of 59 (73%) trisomy 21 fetuses and in three of 603 (0.5%) fetuses with normal karyotype.

在59名第21对染色体三体的胎儿中有43名(73%)出现了鼻骨缺失,在正常染色体组合的603名胎儿中有3名(0.5%)出现鼻骨缺失。

Results During the period of January 2003 to March 2005,21 924 fetuses were scanned in our hospital and 11 fetuses with omphalocele were detected including 6 miss-diagnosed.

结果2003年1月至2005年5月间我院共进行了21 924例胎儿超声筛查,其中脐膨出11例,超声检出5例,漏诊6例。

The lungs of CDH fetuses were hypoplastic compared with normal controls and had thick walled septa with poorly developed saccules. In contrast, Dex and EGF fetuses showed improved mesenchymal differentiation with well-developed saccules and thin-walled septae, manifested by radial alveolar counts , mean terminal bronchiole density , ratio of pulmonary mesenchyma and that of pulmonary acinuses.

EGF组肺组织形态明显改善,表现在肺泡间隔变薄,肺泡面积所占比例明显增大,轴向腺泡计数较CDH组及Dex组均有明显增加,其平均终末支气管密度较CDH组改善明显,但仍少于正常对照组。

Results The parietal bone of the 9th-week fetuses was a layer of mesenchyme membrane with positive BMP immunoreactivity. In tile parietal hones of 10th to 36 week fetuses, BMP positive immunoreactivity was found in osteoblasts, newly-born osteocytes and periosteum.

结果 第9 w胎儿顶骨为一片间充质膜,呈BMP阳性反应;第10~36 w胎儿顶骨内BMP阳性反应主要见于成骨细胞、新生骨细胞及骨膜中。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%,0 and 38% respectively in sorted GPA positive cells of 3 samples by FISH,and the results of GPA+ cells of the 3 cases by PCR were positive,negative and positive respectively,which were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证实。

Methods Monoclonal antibody against the glycophorin A was used to identify nucleated erythrocytes in the peripheral blood of pregnant women.Candidates fetal cells from 3 pregnancies at 16 to 24 weeks of gestation were isolated successfully.Sorted cells were also confirmed using Y-special probe (PY3.4) repeated sequences in maternal blood from women carrying male fetuses were detected by polymerase chain reaction.Results The PY3.4 position hybridization rates were 31.5%0 and 38% respectively in sorted GPA positive cells of 3 samples by FISHand the results of GPA+ cells of the 3 cases by PCR were positivenegative and positive respectivelywhich were manifested by the gender after aborticide.

选用红细胞特异单克隆抗体Glycophorin A结合流式细胞术成功地从3例妊娠16~24周的孕妇血中分离出NRBCs,并用Y-染色体特异重复序列DNA探针(PY3.4)荧光原位杂交及聚合酶链反应技术检测Y-染色体特异序列结果 FISH结果,3例分选出的GPA阳性细胞中PY3.4杂交阳性率分别是31.4%、0、38%;其PCR结果,分别为阳性、阴性、阳性,并在引产后得到性别证明。

And intentionally impregnating women and aborting their fetuses?

和故意浸渍妇女和流产胎儿?

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