查询词典 femurs

Methods: BMSCs were harvested from the femurs, tibias and humerus bones of the rabbit, then separated, purified and proliferated.

采用体外细胞培养技术自兔股骨、胫骨及肱骨中分离BMSCs进行纯化、培养。

Methods: MSCs were isolated from adult inbred Wistar rats' femurs and tibias, cultured in high glucose Dulbecco's modified eagle medium with 20% fetal bovine serum, in 37 C, 5% CO2 condition.

采用成年近交系Wistar大鼠，分离双侧胫股骨全骨髓细胞，于20％FBS的高糖型DMEM培养基、37℃、5％CO_2条件下培养。

Methods Bone marrow cells were flushed from femurs and tibias of healthy Lewis rats, depleted of erythrocytes with osmotic lysis.

方法大鼠骨髓细胞经贴壁去除悬浮细胞，加入细胞因子(IL-4和GM-CSF)培养2周。

Methods BMSCs were harvested from the femurs, tibias and humems bones of the rabbit, then separated, purified and proliferated.

采用体外细胞培养技术自兔股骨、胫骨及肚骨中分离BMSCs进行纯化、培养。

METHODS: Lipofectamine method: The BMMSCs were obtained from the tibias and femurs of the guinea pigs. The third passage BMMSCs were cultured with plasmid-lipofectamine mixture for 6 hours, followed by fetal bovine medium for 48 hours. Immunohistochemistry was performed for transient expression. G418 was added after 48 hours.

①脂质体法：取体外分离、培养的第3代豚鼠骨髓间充质干细胞，将质粒-脂质体混合物加入含细胞的培养基中培养6 h，再加入胎牛血清的培养基，孵育48 h 后行免疫组织化学检测，即为瞬时表达。48 h后加入含G418培养基筛选。

All 38 cases of floating knee fracture were treated with locking nail fixation .Different parts of the fracture were treated with different locking nail internal fixation, 38 tibias, 15 femurs fractures were treated with open reduction and antergrade internal locking nail fixation.

对38例&浮膝&骨折病例均使用双交锁髓内针内固定治疗，根据不同骨折部位采用不同髓内针内固定，其中38例胫骨、15例股骨骨折切开复位，顺行髓内针内固定，13例股骨骨折骨采用逆行髓内针固定。

Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats. MSCs were cultured, proliferated and purified by passage culture. After being induced by bFGF, cultuered MSCs were divided into experimental groups (GM1 group, GDNF group and GDNF+ GM1 group) and control group.

取雄性Wistar大鼠股骨和胫骨骨髓，进行MSCs的体外培养、传代扩增及纯化。bFGF预诱导24 h后，依据加入的神经营养因子不同分为单唾液酸四己糖神经节苷脂（GM1）组、胶质源性神经营养因子组和GDNF+GM1组，以及对照组。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。