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Using semi-quantitative RT-PCR, the differential expression profiles of eleven selected genes were confirmed in the ovaries of triploid and diploid. These genes fell in gene categories with a wide range of functions. The results indicated that triploidy affects the dynamic gene regulatory network in triploid ovary. This study established a firm basis for future investigation on characterization of crucial molecular events for normal ovarian development in shrimp.To further dissect exact gene functions for gonad development of shrimp, three differentially expressed genes between diploid and triploid ovary, PCNA (proliferating cell nuclear antigen), CAS/CSE1 (cellular apoptosis susceptibility protein/chromosome segregation 1) and SSRF (spermatogonial stem-cell renewal factor) were characterized on certain aspects.

利用抑制性消减杂交技术，建立了对虾二倍体和三倍体卵巢间的2个消减文库；在正向消减文库(以三倍体卵巢作为实验组，二倍体卵巢作为驱动组)中，鉴定到54个基因；在反向消减文库(以二倍体卵巢为实验组，三倍体卵巢为驱动组)中，鉴定到16个基因；选取11个差异表达的基因，利用半定量RT-PCR的方法对其在二倍体和三倍体卵巢间的表达进行了检测，均能很好地与消减结果相吻合；这些差异基因编码多种功能的蛋白，分析表明染色体的三倍化使三倍体卵巢中的基因调控网络受到了影响；为深入揭示维持卵巢正常发育的关键分子调控事件奠定了基础。

I learn'd from it also this in particular, that being abroad the rainy Season was the most pernicious thing to my Health that could be, especially in those Rains which came ended with Storms and Hurricanes of Wind; for as the in which came in the dry Season was always most accompany'd with such Storms, so I found that Rain was much more dangerous than the Rain which fell in September and October.

现在，闲话少说，重回到日记上来吧。我当前的境况是：虽然生活依然很艰苦，但精神却轻松多了。由于读《圣经》和祈祷，思想变得高尚了，内心也有了更多的安慰，这种宽慰的心情我以前从未有过。同时，健康和体力也已恢复，我重又振作精神，安排工作，并恢复正常的生活。

He lived in Argentina as a boy and fell in love with the local food, the main ingredient of which is meat: it's among the best in the world and is prepared in a variety of ways: barbecued, skewered, grilled or boiled, always served in generous portions accompanied by pulses, potatoes, rice or maize and various sauces such as chimichurri.

他在阿根廷居住的时候还是一个孩子，并且喜欢上了当地的食物，这些食物的主料就是肉，这是世界上最好的肉，并且有很多方法来制作：野外烤、烤肉串、一般烧烤或是煮，通常提供足够的配料来吃，例如豆类、西红柿、大米、玉米和各种各样的酱，例如 chimichurri 。

Day, he one when hunting in the forest,he caughtsight of artemis'' fair maidens,the seven pleiades .hewas so attracted by their beauty that he started chasing them.indespair the charming maidens prayed to artemis for help and were sent up by the goddess to the heavens as a constellation .later he fell in love with another maiden,merope by name,butrash and impatient,he took the maiden by force and enraged her father,king oenopion of chios.the king made him drunk and put out his eyes .following the sound of the cyclopes'' hammer,he went to hephaestus,who sent a guide to travel with him to the east,where the sun rose.exposing his eyeballs to the rays of the sun,he was restored to his eyesight.he became aconstant companion of artemis and followed the goddess in the chase with his dog sirius.apollo did not approve of this love, however.one day he dared his sister to shoot at a dark spot moving in the sea.little did she suspect that she was aiming herarrow at orion,for the dark spot turned out to be the head of orion swimming in the ocean.artemis was extremely sad.as she could not pull him back from the grasp of death she sent him up to the sky as a constellation,with his hunting dog sirius be sidehim.

他是海神波塞冬漂亮的儿子。一天，他在森林中打猎，看到了阿尔特弥斯的仙女们——七颗明星。他如此爱慕她们的美丽，便追赶她们。困境中，美貌的仙女们向阿尔特弥斯求救。结果女神将她们都送入天空充当星座。后来奥利安爱上了另一少女墨洛珀。他鲁莽急躁地施暴于她。这激怒了少女的父亲俄诺皮耳国王。国王把他灌醉后，挖出了他的眼睛。随着&独眼巨人&大锤的敲响，他来到火神赫斐斯托斯跟前。火神派向导带他去太阳升起的东方。将眼球暴露于阳光后，他就恢复了视力，成了女神阿尔特弥斯永久的伴侣，并带着他的猎狗天狼跟随女神打猎。阿波罗并不赞成他们之间这种恋情。一天，阿波罗怂恿阿尔特弥斯向海中一移动的黑点射箭。她根本没想到自己是将箭瞄准奥利安。当看清黑点竟是在游泳的恋人奥利安的头时，阿尔特弥斯悲痛万分。因她无力使他起死回生，只好将他送入天空作一星座。他的猎狗天狼则蹲在他的身旁。

Orion orion was a giant hunter.he was the beautiful son of poseidon.one day ,when hunting in the forest,he caughtsight of artemis'' fair maidens, the seven pleiades .hewas so attracted by their beauty that he started chasing them.indespair the charming maidens prayed to artemis for help and were sent up by the goddess to the heavens as a constellation .later he fell in love with another maiden ,merope by name,butrash and impatient, he took the maiden by force and enraged her father,king oenopion of chios.the king made him drunk and put out his eyes .following the sound of the cyclopes'' hammer,he went to hephaestus ,who sent a guide to travel with him to the east,where the sun rose.exposing his eyeballs to the rays of the sun ,he was restored to his eyesight.he became aconstant companion of artemis and followed the goddess in the chase with his dog sirius.apollo did not approve of this love,however.one day he dared his sister to shoot at a dark spot moving in the sea.little did she suspect that she was aiming herarrow at orion ,for the dark spot turned out to be the head of orion swimming in the ocean.artemis was extremely sad.as she could not pull him back from the grasp of death she sent him up to the sky as a constellation, with his hunting dog sirius be sidehim.

奥利安奥利安是位身材高大的猎手。他是海神波塞冬漂亮的儿子。一天，他在森林中打猎，看到了阿尔特弥斯的仙女们——七颗明星。他如此爱慕她们的美丽，便追赶她们。困境中，美貌的仙女们向阿尔特弥斯求救。结果女神将她们都送入天空充当星座。后来奥利安爱上了另一少女墨洛珀。他鲁莽急躁地施暴于她。这激怒了少女的父亲俄诺皮耳国王。国王把他灌醉后，挖出了他的眼睛。随着&独眼巨人&大锤的敲响，他来到火神赫斐斯托斯跟前。火神派向导带他去太阳升起的东方。将眼球暴露于阳光后，他就恢复了视力，成了女神阿尔特弥斯永久的伴侣，并带着他的猎狗天狼跟随女神打猎。阿波罗并不赞成他们之间这种恋情。一天，阿波罗怂恿阿尔特弥斯向海中一移动的黑点射箭。她根本没想到自己是将箭瞄准奥利安。当看清黑点竟是在游泳的恋人奥利安的头时，阿尔特弥斯悲痛万分。因她无力使他起死回生，只好将他送入天空作一星座。他的猎狗天狼则蹲在他的身旁。

Orion was a giant hunter.he was the beautiful son of poseidon.one day,when hunting in the forest,he caughtsight of artemis'' fair maidens,the seven pleiades .hewas so attracted by their beauty that he started chasing them.indespair the charming maidens prayed to artemis for help and were sent up by the goddess to the heavens as a constellation .later he fell in love with another maiden,merope by name,butrash and impatient,he took the maiden by force and enraged her father,king oenopion of chios.the king made him drunk and put out his eyes .following the sound of the cyclopes'' hammer,he went to hephaestus,who sent a guide to travel with him to the east,where the sun rose.exposing his eyeballs to the rays of the sun,he was restored to his eyesight.he became aconstant companion of artemis and followed the goddess in the chase with his dog sirius.apollo did not approve of this love,however.one day he dared his sister to shoot at a dark spot moving in the sea.little did she suspect that she was aiming herarrow at orion,for the dark spot turned out to be the head of orion swimming in the ocean.artemis was extremely sad.as she could not pull him back from the grasp of death she sent him up to the sky as a constellation,with his hunting dog sirius be sidehim.

奥利安是位身材高大的猎手。他是海神波塞冬漂亮的儿子。一天，他在森林中打猎，看到了阿尔特弥斯的仙女们——七颗明星。他如此爱慕她们的美丽，便追赶她们。困境中，美貌的仙女们向阿尔特弥斯求救。结果女神将她们都送入天空充当星座。后来奥利安爱上了另一少女墨洛珀。他鲁莽急躁地施暴于她。这激怒了少女的父亲俄诺皮耳国王。国王把他灌醉后，挖出了他的眼睛。随着&独眼巨人&大锤的敲响，他来到火神赫斐斯托斯跟前。火神派向导带他去太阳升起的东方。将眼球暴露于阳光后，他就恢复了视力，成了女神阿尔特弥斯永久的伴侣，并带着他的猎狗天狼跟随女神打猎。阿波罗并不赞成他们之间这种恋情。一天，阿波罗怂恿阿尔特弥斯向海中一移动的黑点射箭。她根本没想到自己是将箭瞄准奥利安。当看清黑点竟是在游泳的恋人奥利安的头时，阿尔特弥斯悲痛万分。因她无力使他起死回生，只好将他送入天空作一星座。他的猎狗天狼则蹲在他的身旁。

But in 2008, stocks fell in lock step both at home and abroad, while in 2009 they rose almost in unison around the world.

但在2008年，国内外股市同步下跌，2009年，全球股市又几乎是不约而同地上涨。

It was observed as follows:(1)The body temperatureof the cord transectedanimals fell in a good agreement with ambient temperature.It appears thatthe fall in B.T.is one of the main causes inducing ulceration.(2)Administrationof epinephine decreased ulceration and prevented the fall in B.T.both in a dose-dependent manner.(3)Cord transection induced ulcer formation was markedlyattenuated by adrenalectomy and exacerbated aga...

结果表明：(1)脊髓横断动物的体温随环境温度而下降，体温的下降可能是诱发溃疡的重要原因之一；(2)注射肾上腺素可减轻溃疡和防止体温下降，且两者均呈剂-效关系；(3)切除肾上腺可使脊髓横断诱发的溃疡明显减轻，如补充注射地塞米松则可使之重新加重，但体温的下降在以上情况下均不受影响；(4)脊髓横断后胃粘膜泌酸和内分泌功能的改变可能与溃疡生成有关。

In fact, is very single-minded, and they also know how to appreciate are very talented girls who like boys loaded ha ha Shuai like to say that they like to say how great they are, what, but they never admit that he or beautiful or narcissistic Would like to arouse the attention of what it is because they thought it was a way of self-love, if people do not even know how to self-love, then to others so that people are concerned about the hypocrisy, some wear untrue, but very cautious The decision to make, very polite to strangers, very friendly acquaintance, is a simple, lively, gentle, with wisdom, sense of responsibility of the girls, very tolerant, sometimes speak with a forked tongue yo ha ha ' Difficult to fall in love with a male, but if you fell in love with will never forget, although in the minds of many boys like, but there is nothing more than good dim, for a time-release mentality and I said, because they would like from the bottom of my heart To appreciate others, it tends to mislead some of the boys, only one cow was angry that his temper will not be jealous of others, the ability to judge, some dependent on the boyfriend, but will not have any boyfriend will be difficult to help them, and the like And the co-operation, the money is very sensitive to some inner melancholy, like the quiet, simple world, and often unsatisfactory, but is still able to adapt.

其实是很专一的，而且她们也都是懂得欣赏很才华的人的女生喜欢装男生哈哈喜欢说自己帅喜欢说自己是多么的伟大什么的，但她们从不承认自己很漂亮或者自恋或者想引起别人的注意什么的，那是因为她们认为那是自爱的一种方式，如果人连都不懂得自爱的话那么那人给他人的关心都是虚伪的，不真实的有些磨，但是很谨慎做出的决定，对陌生人很礼貌，对熟人很友善，是个单纯、活泼、温柔、拥有智慧、有责任感的女生，很宽容，有时会口是心非哟{哈哈'很难爱上一个男生，但是如果爱上了就会刻骨铭心，虽然在心中喜欢很多男生，但也只是朦胧的好感罢了，过一阵子心态又放平了我说过，因为她们从心底就很喜欢欣赏他人，所以往往会误导某些男生，只是一生气那真是牛脾气，不会嫉妒别人，判断力强，对男友有些依赖，但是不会有什么困难就要男友帮她们的，而且很喜欢与人合作，对金钱很敏感，内心有些忧郁，喜欢安静、简单的世界，却往往不如意可是依旧可以适应。

In 1827 Berlioz fell in love, wildly, passionately, dramatically of course, with an English actress, Harriet Smithson, then performing in a season of Shakespeare plays in Paris.

1827年，柏辽兹狂热地、激情地、当然也是戏剧性地爱上了一位英国女演员哈里特·史密森。当时她正在巴黎的莎土比亚戏剧季节中演出。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。