查询词典 far-sighted

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

In the passage the tightly shutting door opened and closed again, and a tempest of applause was blown as far as the greenroom.

朝向走廊的带软垫的门开了一下，又关上了，一阵暴风雨般的掌声一直传到演员休息室里。

I want to ask you if this bus goes down Fifth Avenue as far as Greenwich Village .

我想问一下这车是否沿15大道直至格林威治村。

I want to ask you if this bus goes down Fifth Avenue as far as Greenwich Village.

等一下，这车是不是经过第五大道，一直到格林尼治村那儿？

Wait. I want to ask you if this bus goes down Fifth Avenue as far as Greenwich Village .

等一下，这车是不是经过第五大道，一直到格林尼治村那儿？

And the change that gregarious website brings for our life is far more than still these.

而社交网站为我们生活带来的改变还远远不止这些。

Gretchen : I'm not far. Are you okay?

我就在附近，你还好吗？

And the consequences could be far more serious for anyone using their home internet connection for business purposes, said Simon Halberstam, head of e-commerce law at Sprecher Grier Halberstam..

为此，你不得不向调查此事件的警察表示你的清白，这整个过程是令人头痛的。

I am grimalkin: Still be a person so far do.

我是老猫：到目前为止还是一个人做。

I am grimalkin: Capital investment is done not have however too much, but the investment of energy is far costlier than capital.

我是老猫：资金投入倒是没有太多，但精力的投入远比资金更值钱。

No complex distressful things on your mind but only our love and care for each other.

无复杂痛苦的事情，对你的心，但只有我们的爱和照顾对方。

At first light, on his own initiative, Vick located the enemy emplacements andfired on them, using 15 rounds.

破晓时分，维克主动地去查找敌军的炮位并向它们开火，打了15发弹药。