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The dissertation focuses on performance gradient estimation and SRO algorithm of DES aswell as other related topics,such as modeling of DES and discrete event simulation,performancemeasure Hessian matrix estimation,Stochastic Approximationand so on.General Semi-Markov Processis adapted to describe behaviour of DES and discrete eventsimulation.A general result is obtained and applied to analyze strong consistency of performancegradient Perturbation Analysisestimator.Conditions for effectiveness of InfinitesimalPerturbation Analysisare also discussed.New PA algorithms,such as server PA,PA usingDirac generalized function,job sequence PA etc.,are proposed to deal with discreteparameters.Furthermore,strategies of combining PA algorithms with certain iterative rules areanalyzed widely in order to optimize DES parameters and some SRO algorithms are constructed toslove DES optimization problems.

论文将DES性能分析领域中性能梯度估计和单运行仿真优化作为主要的研究对象，探讨了与性能梯度估计有关的DES和离散事件仿真过程建模、高阶导数估计以及随机逼近等理论和方法；建立了具有一般性的DES和离散事件仿真的广义半Markov过程模型；获得了分析性能梯度估计量强相合性的理论结果和无穷小摄动分析算法应用的条件；提出了诸如服务台数目的摄动分析、奇异摄动分析、串联生产线和加工车间中工件加工序的摄动分析等一系列摄动的思想和算法，对连续参数和离散参数都进行了深入的研究；对摄动分析算法同优化规则结合实现仿真优化的策略也进行了广泛的分析，构造了新型的变界截尾的随机逼近算法，并用于DES的单运行仿真优化研究；最后，给出了DES性能分析方法的实证性研究结果。

In order to analyse the structural reliability by stochastic finite element method rapidly and efficiently, a method combined with finite element method and gradient optimum algorithm based on ANSYS is presented when referring to the geometric interpretation of structural reliability index.

为快速、高效地进行基于随机有限元法的结构可靠度分析，从结构可靠性指标的几何意义出发，提出了在ANSYS平台上采用有限元法和梯度优化算法相结合的方法进行可靠度分析的构想，对ANSYS进行二次开发实现了该方法。

Several methods of deriving horizontal wind fields from satellite temperature data are analyzed and compared in this paper, including the calculation methods of geostrophic wind, gradient wind and balance wind.

本文分析和比较了利用卫星温度资料计算水平风场的方法，包括地转风、梯度风和平衡风的计算方法。

Produced the height-gradient form of the geostrophic velocity equations and provided many examples of geostrophy by looking at 500mb height maps.

推导出地转速度方程的高度梯度形式，藉由观察500百帕高度地图举出很多地转的例子。

Methods:Via a cannula iserted into adominal aorta, Gey's balanced salt solution containing 0.025% collagenase P was perfused into rat normal pancreas. After digestion with collagenase P and protease, Nycodenz density gradient centrifugation was performed to isolate PSC. PSC was identified by its morphology, cytoplasmic lipid droplets and immunocytochemical staining for desmin, GFAP and α-SMA. Results:The production,viability and purity of isolated PSC were（15.3 ± 4.6）× 103／g body weight,（95.0 ± 3.5）%, and ﹥80％ respectively.

大鼠腹主动脉插管灌注后，经胶原酶消化、Nycodenz密度梯度离心，分离得到PSC；通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白、神经胶质酸纤维蛋白（glial fibrillary acidic protein，GFAP）、α平滑肌肌动素（α-smooth muscle actin，α-SMA）的表达来鉴定PSC。

In this paper the convergence rate and the global convergence properties of the Gradient Method are studied.

本文研究了梯度法的收敛速度和全局收敛性。

Rice seed storage glutelin could be separated into three acidic and three basic subunit components by SDS-PAGE using 15%~25% gradient acrylamide gel.

利用15%～25%丙烯酰胺梯度凝胶的SDS-PAGE分析法可将水稻种子贮藏谷蛋白分离为3个酸性亚基和3个碱性亚基。

Regarding the circular defects such as leakage gnarl in log, this paper designed an improved Laplace's special gradient method to clarify the target image.

针对原木中的漏节等圆形缺陷，本文设计了改进的拉普拉斯算子法增强图像中目标的清晰度。

Regarding the circular defects such as leakage gnarl in log, this paper designed an improved Laplaces special gradient method to clarify the target image.

针对原木中的漏节等圆形缺陷，本文设计了改进的拉普拉斯算子法增强图像中目标的清晰度。

In this paper,we developed a color to gray method based on gradient domain .

本文提出了一种基于梯度域的彩色图像转灰度图像的方法。

The use of the web to spread fears that flicking the switch on the LHC could create a Black Hole that could swallow up the Earth particularly concerned him, he said.

他说，使用互联网散布恐慌说启动LHC会生成一个可能吞噬地球的黑洞的做法让他感到忧虑。

Not a single word have I written down.

我一个字也没有写下来。