查询词典 fall into a pattern

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Hey yo jim man why don't ya, you don't you kick some of that you know you know how you do it man it's a trip people don't even believe were together right now but tell your story you know the one i like say it for me riders on the storm riders on the storm into this house we're born (into this house we're born) into this world we're thrown (into this world we're thrown) like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah goin' off of this off of that with the lizard king bumpin' in the back how bout that driftin', liftin', swiftin', coastin', testaroastin' but the wheels won't stop 200 on the highway fresh up off the block he's a rider, na he's a killer dresses in all black but his hat says stealla pedal to the metal i gotta go hard drive by and say hello hey fredwreck you my mello now let me here what i sound like acapella wow ride dip swish now bring it back just like this like a dog with out his bone unlike a g with out his chrome it's hard to imagine the homey dog in a jag and he's checkin' for the checkered flag comin' in first never in last cause my car to fast i neva eva run out of gas cause i just to clean i do it upper class so fasten your seat belts its so hot it will even make heat melt so get a bowl and roll and ride slip through the slip and slide like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah need for speed i'm trying to take the lead hold on little homey before you run into the trees i've seen thinks that i would have never saw before hey yo jim let'em in, let'em in open up my back tire smokin' the whole street and now the police wanna flash there lights and chase the dogg all night but i won't pull over nor give up cause i just don't give a fuck yeah from the side boy where we was born and raised straight up to ride boy continuously, we get to an expeditiously keep the light on east side on snoop dogg and the doors and yeah we bout to ride on riders on the storm riders on the storm into this house we're born into this world we're thrown were thrown

嘿摇摇弯轨机人为什么不唉，你不你踢一些那你知道你知道如何你这样做人这是一次旅行人们甚至不相信是在一起此时但告诉你的故事你知道那个我像为我说它骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔像个狗没有一根骨头一个演员那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀去'离开对于这离开关于那和蜥蜴国王 bumpin'在后面如何拳击比赛那 driftin'，liftin'，swiftin'，coastin'，testaroastin'但是这个车轮不会停止两百在公路上新鲜向上远离块他的一个骑士，娜他的一个杀手衣服在纯黑但他的帽子说stealla 踩踏板金属我有去难驶过和问好嘿fredwreck 你我的梅洛现在让我这里什么我听起来像acapella 哇骑现在倾斜豪华带来它回来只是这样像个狗与出来像个狗没有一根骨头一演员借出的骑士在暴风雨上那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀需要速度我是试图着先鞭继续小像家一样在你未碰撞树木我已经看到想那我从来不锯在以前嘿摇摇弯轨机让'em 在，让'em 在开发我的后面疲劳smokin'一街一巷那么警察要闪光那儿灯光和追逐道格整夜但我不会拦截也不放弃造成我只是不给一个性交是呀自边男孩我们在那里出生和提升直直至骑男孩骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔我的翻译不太标准别见怪

Meet fishes, they can't immediately hurtle up to rob a food, but class action, rush through the fishes into a ring of encirclement, then take turns into a ring of encirclement to take a meal;If meet penguin to stay in not and greatly floating ice, the killer whale will jump to float in the ice and make to float ice occurrence inclination, so the penguin will fall into its big mouth;For presume to contend with them of walrus, killer whale will be divided into two brigades, from the positive false attack, 1 brigade's another brigade from back attackstone, keep killing walrus corpse the horizontal time is wild;Meet other large cetaceanses, the killer whale then will gather but Jian it, lay siege to opponent together;Sometimes they even will adopt "Zha dead" strategy and launch to suddenly assault.

遇到鱼群，它们不会立即冲上去抢食，而是集体行动，把鱼群赶入到包围圈中，然后轮番进入包围圈进食；如果遇到企鹅待在不大的浮冰上，虎鲸会跃到浮冰上，使得浮冰发生倾斜，这样企鹅就会落入它的血盆大口之中；对于敢于与它们抗争的海象，虎鲸会分成两队，一队从正面佯攻，另一队从背后攻击，直杀得海象尸横遍野；遇到其他大型鲸类，虎鲸则会聚而歼之，一起围攻对手；有时它们甚至会采用&诈死&策略，发动突然袭击。谁帮我把这段故事翻译成英语的，谢谢了、明天就要了，好急啊我的财富值剩不多了，所以，还请大家见谅

Meet fishes, they can't immediately hurtle up to rob a food, but class action, rush through the fishes into a ring of encirclement, then take turns into a ring of encirclement to take a meal;If meet penguin to stay in not and greatly floating ice, the killer whale will jump to float in the ice and make to float ice occurrence inclination, so the penguin will fall into its big mouth;For presume to contend with them of walrus, killer whale will be divided into two brigades, from the positive false attack, 1 brigade's another brigade from back attackstone, keep killing walrus corpse the horizontal time is wild;Meet other large cetaceanses, the killer whale then will gather but Jian it, lay siege to opponent together;Sometimes they even will adopt "Zha dead" strategy and launch to suddenly assault.

遇到鱼群，它们不会立即冲上去抢食，而是集体行动，把鱼群赶入到包围圈中，然后轮番进入包围圈进食；如果遇到企鹅待在不大的浮冰上，虎鲸会跃到浮冰上，使得浮冰发生倾斜，这样企鹅就会落入它的血盆大口之中；对于敢于与它们抗争的海象，虎鲸会分成两队，一队从正面佯攻，另一队从背后攻击，直杀得海象尸横遍野；遇到其他大型鲸类，虎鲸则会聚而歼之，一起围攻对手；有时它们甚至会采用&诈死&策略，发动突然袭击。谁帮我把这段故事翻译成英语的，谢谢了、明天就要了，好急啊我的财富值剩不多了，所以，还请大家见谅问题补充：不要在线翻译的呀。。。。。这样怎么见人啊。。。

In this paper, the mathematic model of the 3D garment basic pattern is divided into geometry model and analytical model, the former decides the fundamental structure of garment basic pattern surface, but the latter presents the analytical expression.

考虑到服装设计的特殊性，文中将三维服装原型的数学模型区分为几何模型与解析模型两类，前者确定了服装原型曲面的基本结构，后者给出了解析表述。

It and the folk paper-cut is similar to paper-cut difference ismostly in the colored paper with scissors to cut paper into avariety of bird fleas with skilled, fortune and longevity Hi,characters landscape, etc. for people to buy to put up orappreciation, while the shoe-like pattern is a piece of paper withthe burin Yitabaixuan variety of plants that pattern down, lacepattern, designed for families on behalf of women as a kind ofembroidery.

它和民间剪纸类似，不同的是剪纸大多是用剪刀在有色纸上用巧手剪出各种花鸟鱼虫、福禄寿喜、人物风景等，供人们买去张贴或欣赏，而花样鞋样是在一沓白宣纸上用刻刀纹下来的各式各样奇花异草、花边图案，专供家庭妇女绣花的一种替样。

Cairngorm Framework uses MVC pattern as its main design pattern, and divided Flex application into three parts: view, model and control. The view is responsible for displaying user interface. The model is focused on data keeping, especially keep data consistency in two facts, one is data consistency between client and server, the other is between different views sharing the same data.

视图层主要负责展示用户界面；模型层则关注于数据的保存，尤其是在两个方面维护数据的一致性，一是Flex客户端和服务器端之间的数据一致性，二是Flex不同视图对同一数据引用的一致性；而控制层除了将模型里的数据展示到视图中之外，还关注于事件的处理和服务的调用，为之提供了一整套封装的机制。

Weft distribution pattern and its input of fabric with varied weft density ;2. In ordor to weave the varied weft density fabric of which weft distribution pattern is prescribed in sections,the definition field of fabric′s weft density function is divided into continuous and discrete intervals according to the variation characteristics of weft density of fabric.

为织造出分区段设定纬密变化的疏密纬织物，根据织物的纬密变化特点，把织物纬密函数的定义域分成连续区间和离散区间；在此基础上，对于分段连续且可能存在间断点的连续区间，建立了卷取量方程；分析并确定了织物各纬卷取量的解区间，给出了卷取量的求解方法——以解区间足够小为解判据的二分法，并阐明了求解方法在间断点处的有效性；对于由离散点构成的离散区间，给出了区间端点卷取量的取值方法和区间中各纬卷取量的确定方法。

It is iffy that distinguished the evolvement of a institution into cause pattern and force pattern.

以往把制度演化分为诱致式和强制式是有问题的。

The findings are divided into three parts: First, through the archetypal pattern, we see the intercommunity and the intercommunion of human mind, and we also see the permanence and the universality of human nature, therefore the reproduction of archetype in literary work is not only the repetition of fixed literature content in pattern frame, but also the representation of intercommunion and intercommunity in human psychologically emotional activities.

研究结果分为三部份：第一、通过原型的模式，既看到人类心灵的共同性、相通性，也看到人性的永恒性与普遍性，所以文学作品中原型的重现，不只既定的文学内容在模式框架中的重复，也是人类心理情感活动中相通性与共同性的表现。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48