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And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠，雌雄不限(中国医科大学实验动物中心提供)，腹腔注射10%水合氯醛0.4ml/100g，无菌条件下取出气管，无菌PBS反复冲洗，洗净血液和粘液，置于DMEM/F12培养液中(含10%胎牛血清)，于培养液中加入终浓度为100mg/ml的5-Fu，37℃，5%CO2孵育12小时，弃去上述培养液，换成新鲜DMEM/F12液(含10%胎牛血清)，同时加入终浓度为200nM的TSA继续培养，于换液后3、6、12、24、48小时分别10%中性福尔马林固定，石蜡包埋，制成4μm厚的组织切片。

There were packmen's yawps 、 buyer's argy-bargy 、 children's laugh and sounds of gong 、 drum and firecrackers.文汇博客f!O6zY3pLx_H1

在堶情A有小贩的叫喊声，顾客的讨价还价声、小朋友的欢笑声和锣鼓鞭炮声。

There were packmen's yawps 、 buyer's argy-bargy 、 children's laugh and sounds of gong 、 drum and firecrackers.文汇博客$XV4?1'3P I;F

在堶情A有小贩的叫喊声，顾客的讨价还价声、小朋友的欢笑声和锣鼓鞭炮声。

The good news is that it's extremely unlikely that there's anything physically wrong with him, because if a guy's hurting he doesn't raise his arm slot like Wang did last night, he drops it.7 ~3 q9 1 f l$ T** h

好消息是，他极度不可能有任何身体方面的问题，因为，假如某人有伤的话，他的手臂会垂下来，出手的手臂角度不可能像小民昨晚那样。

Apart from its unique performance properties and environmental benefits the Victrex PEEK-based honeycomb provides numerous other advantages including excellent impact resistance; unlike an aluminium or thermosetting honeycomb, PlastiCell's honeycomb will not remain deformed by accidental impacts during machining or assembly of the part and, due to the material's high temperature performance, the honeycombs can operate continuously up to 220°C (428°F, a thermoformable honeycomb core allows for the production of parts with short cycle times using an autoclave-free process. There is no size constraint as it is possible to make an endless honeycomb using PlastiCell's manufacturing process. Part size limitation is dictated by the hydraulic press platen size.

除了其独特的性能特性和环境效益外，VICTREX聚醚醚酮基蜂窝芯材还有很多其它优点，比如优良的耐冲击性，不像铝或热固性蜂窝芯材，PlastiCell蜂窝芯材不会在部件加工或安装期间由于受意外撞击而变形，因为该材料具有高温性能，（此蜂窝芯材的操作温度可连续达到220℃（428℉），生产热压成型的蜂窝芯材的周期短，不使用高压反应釜加工，没有尺寸限制，用PlastiCell工艺可以生产连续蜂窝芯材，液压模板的尺寸限制部件的大小。

Its basic viewpoints are reflected in W.F. Pinar's "reconceptualism", M.Van Manen's phenomenological curriculum study and D. J. Smith's "hermenutical pedagogy".

派纳的&概念重建主义&、范梅南的&现象学课程研究&以及史密斯的&诠释学教育学&反映了其基本主张。

Last spring, Cleveland player Ira Newble asked his teammates to sign an open letter to China pr o t e s ting the country's interests in S u d a n, where the government has sponsored atrocities in the D a r f u r region.

去年春，Cleveland球员Ira Newble让他的队友签署一封给中国的公开信，抗议其在达富尔地区支持暴行，在苏丹的利益。

The tractors are to be delivered by lighter to F.A.S. s/s "ORIENT", Port of London.

4拖拉机将用轮船运交伦敦港&东方&轮，船边交货。

The tractors are to be delivered by lighter to F.A.S. s/s "ORIENT", Port of London.

拖拉机将由驳船运交伦敦港&东方&轮，船边交货。

The fundamental difference between Bakhtin 's ideas about semiology and structuralism semiology with F.D. Saussure and U.M.Lotman as representative is: Bakhtin's semiology is built out of language system, while the latter's semiology is built within the system of language.

巴赫金的符号哲学与以索绪尔和洛特曼为代表的结构主义符号学不同，前者建立在语言体系之外，而后者则建立在语言体系之内。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新