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In anticipation of achieving the above objectives, marine-derived actinomycetes, H2003 and Aspergillus terreus Thom have been screened by using this model, the EtOAC extracts of strain H2003 and Aspergillus terreus Thom showed bioactivities of inhibiting the cell cycle progression and cytoclasis of t...

并采用活性追踪分离方法对一株放线菌和一株真菌的代谢产物活性成分进行了初步研究采用减压硅胶柱层析、凝胶柱层析(Sephadex LH-20)、反相硅胶柱层析和反相制备高效液相等分离手段,从具有细胞坏死活性的放线菌H2003分离得到22个单体化合物。

Davallia mariesii has the hightest protective effects among the aqueous extracts of these three plants on the neuronal oxidative damage induced by 6-OHDA in B35 neuroblastoma cell.

因此,进一步探讨海州骨碎补、鳞轴小膜盖蕨及槲蕨之水抽取物对6-OHDA诱发B35神经细胞氧化损伤之保护效果。

the extracts of dipsacus asperoids with certain concentration can improve the osteoblastic proliferation and differentiation.

续断提取物在一定浓度范围内有促进成骨细胞增殖与分化的作用。

Cells were seeded by 2×104/ml in 96 well cell culture cluster. Afer cultured by the extracts of dipsacus asperoids, the function of osteoblastic proliferation was detected by MTT method.

将传代细胞制成密度为2×10~4/ml的细胞悬液,接种于96孔培养板中,细胞贴壁后用续断甙培养液培养,用MTT法测定成骨细胞的增殖能力。

Cells of successive transfer culture were seeded by 5×104/ml and cultured in 24 well cell culture cluster. After confluenced, the extracts of dipsacus asperoids was applied to cell culture, then the ALP content in the culture fluid was detected.

将传代的细胞制成密度为5×10~4/ml的细胞悬液,接种于24孔培养板中,细胞融合单层后用续断甙培养液培养,测定培养液中ALP的含量。

Fresh algal extracts prepared in 0.5 M HCl were neutralised, purified on a SPE column (RP-18, Merck), lyophilised in the presence of 1 mM dithiothreitol to prevent the formation of disulphide bridges and stored at -/20℃.

食藻提取物中拟定0.5米盐酸被瓦解,纯化的固相萃取柱(RP-18, Merck),lyophilised,在场的1毫米二硫苏糖醇,以防止形成二硫键桥梁和储存在-/20℃。

Hepatocytes were treated with 25 or 50 μg/mL of ethanol or ethyl acetate extracts of Ap or 10 or 20 μM andrographolide, which is the major active diterpene lactone of Ap, for 48 h.

因此,本实验利用大鼠初代肝细胞培养模式,探讨穿心莲对诱发 pi 属麸胱甘硫转移(pi class of glutathione S-transferase, GSTP)的作用。

The antifungal activities of these materials were investigated and build foundation to develop low poison plant antifungal products. In experiment, polyphenols were extracted by reflux ectraction method which applyed petroleum ether to degrease and methyl alcohol solvent. Phenolic hydroxyl were examined and inspected by colour-developing reaction and the content of total polyphenols was determined by UV-spectrophotometry. Screening test were performed to the antifungal activities of polyphenol extraction by the tube dilution method to the selected ten pathogenic fungus such as Dothiorella gregaria, Rhizoctonia solani, Phyllosticta pirina, and the minimal inhibitory concentration were measured. The column chromatography and thin layer chromatography were applyed to separate and analyse cortex extracts of Dracaena cochinchinensi Lour.

实验部分采用以石油醚脱脂,甲醇为溶剂的回流提取法来提取植物多酚,用显色反应检识酚羟基;采用紫外分光光度法对多酚粗体物进行总多酚含量的测定;选取了聚生小穴壳菌、立枯丝核菌、梨叶点霉等10株常见农林业致病真菌,采用试管液基稀释法对多酚粗提取物进行了抗真菌活性筛选试验,并测定其最低抑菌浓度;采用柱层析法和薄层层析法对其中抑菌效果较好的龙血竭进行分离分析,根据其中3种化合物的光谱数据UV、IR、~(13C-NMR、1H-NMR和MS鉴定其结构。

It was suggested that the extracts oil of Artemsia dracunculus L. may be an effective immunomodulating agent.

椒蒿具有增强小鼠非特异性免疫的功能,并对体液、细胞免疫功能有促进作用,提示椒蒿是一种免疫调节物。

A new flavanone from Dryopteris sublaeta;2. Isolation and Structure Identification of a New Flavanone from Dryopteris sublaeta;3. RESULTS Five compounds are isolated from the ether fraction of 70% acetone-extracts,and their structures were identified as(2S)5,7-dihydroxy-6,8-dimethyl flavanone desmethoxymatt.

结果浅裂鳞毛蕨地上部分用体积分数为70%丙酮提取液乙醚萃取部位分离得到了5个化合物,分别鉴定为:(2S)5,7-二羟基-6,8-二甲基二氢黄酮,(2S)5,7-二羟基-6,8-二甲基-4′-甲氧基二氢黄酮,(2S)5,7,2′-三羟基-6,8-二甲基-二氢黄酮,(2S)5,7,4′-三羟基-3′-甲氧基-6,8-二甲基二氢黄酮,(2S)5-羟基-4′-甲氧基-6,8-二甲基二氢黄酮-7-O--βD-葡萄糖苷。

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And discuss some key point of integrated management system design, include hazard identification and risk assessment and risk control in the occupational health and safety management, the measure of satisfaction target of customer, the flawlessness of law system, the integration of the integrated management system and enterprise standardization system, unify lead institution and work orgnization, integreted internal audit and integrated management review.

同时,论述了一体化管理体系构建中的几个主要问题,包括职业健康安全管理中的危险源辨识、风险评价和风险控制、顾客满意度的科学监测、法律、法规体系的完善、一体化管理体系与企业标准化体系的有机结合、统一的领导机构和工作机构、一体化的内部审核和管理评审。

Deepwater Taranaki is investigated for its petroleum potential, using all available seismic data tied to shallow-water wells.

运用与浅水井相关的现有全部震波探测数据考察了深海区塔拉纳基盆地的油藏前景。

If you're huffing and puffing at any point during the run, slow down and make sure you can take deep breathes.

如果你任何时候在跑步过程中气喘如牛,降低速度并且确保你能够深呼吸。