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Firstly, an Iterative Closest Point algorithm is applied for coarse registration. Secondly, gradient feature is extracted from image. Based on the principle of projective transformation, the fine registration is performed by finding the maximum CT gradient projections.

首先基于一种最近点迭代法进行快速粗配准；然后提取图像的梯度特征，根据投影变换原理，采用寻找具有CT最大梯度投影位置的方法进行精配准。

Protein fractions(albumin,globulin,prolamin and glutelin)were extracted from defatted tartary buckwheat flour.

采用Osborne分级分离的方法从苦荞粉中制备得到清蛋白、球蛋白、醇溶蛋白和谷蛋白。

Methods HBV DNA was extracted from the serum of patient with hepatitis B. The X gene was amplified by PCR using the primers with EcoRI and HindⅢ digestion sites, and then cloned into pronucleus expression vector pMAL-C2X, which was detected by EcoRI and HindⅢ digestion and sequence.

从乙肝病人血清中提取HBV DNA；以带有EcoRI和HindⅢ酶切位点的引物，用PCR方法扩增X基因；而后定向克隆到原核表达载体pMAL-C2X上，经酶切鉴定和序列分析；以IPTG诱导X融合蛋白的表达。

The term depletion is used to describe not only exhaustion of a natural resource but also the proportional allocation of the cost of a natural resource to the units extracted.

期间损耗是用来形容，不仅用尽一种自然资源，而且还向单位按比例分配提取自然资源费用。

The genomic DNA was extracted from the peripheral blood of the 16-year-old propositus, female.

突变位点经限制性内切酶分析证实。108名健康献血者作对照。

DLL to a main EXE document, Operation can be extracted directly from the memory (or may choose to release it onto the hard disk) This document can protect your DLL, or other procedures for the use of third parties to avoid being revised.

DLL到一个主 EXE 文件中，运行时可以直接从内存中提取，这样可以保护您的动态链接库文件，避免被第三方程序使用或被其他人修改。

No amplification was observed when using RNAs extracted from Pseudorabies virus and uninfected MDBK cell.

对伪狂犬病毒和未接种病毒进行PCR扩增，结果均为阴性。

The extract is prepared by the method containing the following steps: water and/or organic solvent are used as extractant, and the psoralea fruit extract is extracted and obtained from the seeds of psoralea fruit.

所述提取物是用包括如下步骤的方法制备的：用水和/或有机溶剂作为提取剂，从补骨脂种子中提取得到补骨脂提取物。

MethodsThe K562 cells were treated with PSO extracted from Chinese medicine psoralea fruits in different concentrations plus UVA (360 nm) in different irradiation time or not. Apoptosis ratios, ultrastructure changes and expression of Caspase8 and Caspase3 proteins were detected. The factorial design and analysis of variance were used to analyze the interaction among the factors.

方法用不同浓度的中药补骨脂中提取的PSO和不同照射时间、波长为360nm的UVA作用于K562细胞，检测细胞凋亡率、在电镜下细胞超微结构改变及Caspase8、3蛋白的表达，用多因素方差分析法对定量资料进行统计分析。

The total DNA has been extracted from the fresh leaves of 4 medicinal species of pteridophyte with CTAB, SDS, to compare their effects on DNA extraction. The result shows that the optimum preparation method obtained to extract high-quality DNA lays theoretic and practical base for PCR amplification and primer design, for filtrating related gene of the active elements from leaves of the four pteridophytes.

以四种药用蕨类的叶片为材料，采用改进的CTAB法、SDS法分别对四种蕨类进行了总DNA提取效果的比较分析，结果表明，通过实验获得最佳的DNA提取方法，为PCR扩增，引物设计，为筛选四种药用蕨类中的活性成份的相关基因奠定理论和实践基础。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失