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Total DNA were extracted from the pearl oyster Pinctada fucata tissues preserved at -70℃ and fixed in 95% alcohol, respectively.

分别从保存于-70℃和95%乙醇的合浦珠母贝组织提取总DNA。

Firstly, Dempster-Shafer is developed by not only considering the certainty of the evidence, but also considering the average support of the evidence to different subsets in the assignment framework so that it can give more reliable combination result. Secondly, amplitude ratio feature and Euclid distance of the probability density distribution function in Pearson graph from different temporal SAR images are extracted to present change feathers in different scales. Finally, the improved Dempster-Shafer algorithm is applied to fuse the two different features to detect change information of SAR images.

首先通过综合考虑证据本身的确信度和证据对辨别框架中子集的平均支持度进行证据间的加权合成,改进证据理论对证据的合成,提高其可靠性;然后提取不同时相图像间的散射幅度的对比度和概率密度分布函数在皮尔逊图中的欧式距离,两种特征参数代表了像元级和区域级不同空间尺度下微波遥感图像中关于地表变化的信息;最后将改进的证据理论用于两特征之间的融合处理,得到地表的变化信息。

The results showed:among the 7 isolates, five isolates of HY3、GY1-3、ZJ1-1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示:7个菌株中,其中5个菌株HY3、GY1-3、ZJ1-1、HP1、FC3的菌落形态相同:不规则形状、流体、粘性、不透明、表面光滑;另外2个菌株93B、GN1菌落形态相同:不规则形状、干燥、不透明、表面粗糙;用7个菌株接种剪根桉树苗,发病情况非常明显,而自来水对照实验中桉树苗却不发病;无EPS菌悬液培养桉树剪根苗,发病率也很明显,但是相比原菌液,则发病率有不同程度的下降;从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株,用它们配制菌悬液培养桉树苗,设置剪根和不剪根两个处理,每个处理设置五个重复,数据分析结果显示:对于剪根苗,上部和中部、上部和下部的含菌量有显著的差异,中部和下部含菌量差异不显著;带根苗,上部、中部、下部含菌量彼此之间差异显著;不管是剪根苗还是带根苗,木质部和韧皮部含菌量之间的差异都非常显著;上中下与木韧交互作用中,含菌量差异非常显著;最后筛选出来的强弱2个菌株HY3和93B,它们的纤维素酶活性分别为:1.955ug/和1.288ug/,具有显著的差别;果胶酶的活性分别为:1.325 ug/和1.24ug/,没有显著的差别,而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著,分别为:7.08×10-8ug/cell和5.17×10-8ug/cell。

The expression of banana Lectin gene and Pectate Lyase gene in different tissues and on different ripening stages of fruit was studied. The Total RNA of 12 tissues was extracted from bananas root,leaf,fruit peel and pulp on different ripening stages of post-harvest fruit.

本研究还用RT-PCR的方法对香蕉凝集素基因和香蕉果胶裂解酶基因在香蕉不同组织和果实采后不同成熟阶段的表达情况进行了研究。

Were extracted by different extracting solvents in lab, the results indicated that with the increasing of Mn concentration levels, the speciation of Mn in their roots transform from H2O-extractable species to HCl-extractable species and NaCl-extractable species, showed increases of Mn of oxalate binding and pectate binding or prontein binding in cell wall of roots; Mn of H2O-extractable species were 34.55~50.26% of total Mn in stems and increased with the increasing of Mn concentration levels, which benefited upward transport of Mn; Percentage of Mn of H2O-extractable species were 38.55~44.19% and Mn of HCl-extractable species were 20.66~28.74% to total Mn in leaves, suggesting that most Mn in leaves were the speciation of H2O-solubility organic salts or manganese oxalates. 5. Chlorophyll in leaves of Polygonum hydropiper L.

不同的提取剂对水蓼根、茎、叶的提取结果表明,随着锰处理浓度增加,水蓼根中Mn由水提取态向盐酸提取态和氯化钠提取态转化,说明根细胞壁结合的草酸盐和果胶酸盐或蛋白质结合态的Mn含量随锰处理浓度的增加而增加;在茎中,水提取态锰含量占总锰的34.55-50.26%,且随锰处理的增加其水提取态锰的比例增加,这有利于锰向上运输;水蓼的叶片中,水提取态锰占总锰的38.55-44.19%,盐酸提取态锰占总锰的20.66-28.74%,说明叶片中的锰大部分以水溶性有机盐或草酸锰的形态存在。

After alkaline hydrolysis, the hair sample (10 mg) was extracted with pentane, and analyzed by liquid chromatography-tandem mass spectrometry with electrospray ionization and multiple reaction monitoring.

将10 mg样品碱水解后加入戊烷提取,然后进行LC-MS/MS分析,采用正离子电喷雾电离、多反应监测模式测定,方法的最低定量限为25 pg/mg。

The paper studied on the relationships between H–factor and dissolving rates of main saccharides, ratio of total saccharides and pentose to total solid, and concentration of furfural and hydroxymethylfurfural in hot-water pre-extracted liquor of bamboo under different conditions.

本文研究了不同工艺条件下竹子热水预抽提过程中主要的糖类组分溶出率、总糖和戊糖占抽提液中固形物的比例及糠醛、羟甲基糠醛的生成随H因子的变化规律。

A teasaponin is extracted from the tea seed,and then a well-behaved washing assistant is prepared by the teasaponin blended with sodium perborate .

通过从茶籽饼粕中提取茶皂素,并将其与过硼酸钠进行复配进而制取性能良好的洗涤助剂。

Doctors could not explain why the .32-caliber bullets did not penetrate Pereira's skull and didn't even need to be extracted immediately.

医生也不能解释为什么点32口径的子弹并没有进入她的颅内,而且根本不需将这些子弹马上把取出来。

Effective ingredients were extracted from 5 species of chinese medicinal materials respectively and processed compatibility combination. The toxicology and pharmacology of shuang shen perfusate were studied.

方法]从5种中药中分别提取有效成分,进行配伍组方,时双参灌注液的毒理、药理学进行研究。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。