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Methods The total RNA of leucocyte was extracted 43 allergic vasculitis patients and 24 normal subjects before and after treatment with Trizol reagent and the integrity and concentrations of HLA-DRw53 gene mRNA were determined by agarose gel electrophoresis and UV spectrophotometer after denatured with 1% formaldehyde, the product was transcribed into complementary retroviral synthetic deoxyribonucleic acid, and sequence-specific primers were designed based on the sequences of second HLA-DRw53 gene exon and housekeeping gene glyceraldehyde phosphate-dehydrogenase cDNA.

利用随机引物逆转录合成互补脱氧核糖核酸，根据HLA-DRw53基因第二外显子序列和管家基因人磷酸甘油醛脱氢酶的cDNA序列设计序列特异性引物。检测43例变应性血管炎患者治疗前后与24名正常人HLA-DRw53基因mRNA表达量的变化。

Intramuscular lipids was extracted at the day 0、32、60、160、240 of Jinhua ham,then the content and composition of glyceride、phospholipids and free fatty acid were analyzed.

分别提取0、32、60、160、240d的金华火腿中的肌内脂肪，分析其甘油酯、磷脂、游离脂肪酸的含量和脂肪酸组成。

Extracted the subcutaneous lipids of 0, 32day, 60day, 160day, 240day of Jinhua ham, respectively, analyzed the content and composition of glyceride, phospholipids and free fatty acid .

分别提取0、32d、60d、160d、240d的金华火腿中的皮下脂肪，分析其甘油酯、磷脂、游离脂肪酸的含量和脂肪酸组成。

The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

This desire is unacceptable in the godhead and is extracted from Sophia and excluded from the heavenly realm.

这种愿望是不能接受的神格，是提取索菲亚和排除在天上的境界

There's a gold mine of information out there waiting to be extracted.

有一个信息的金矿在那里等着我们去挖掘。

Hydrastine(97.7%) was obtained with extracted rate 65%by extracting the Goldenseal with mixed Solvent;Berberine hydrochloride (99.9%) was obtained by adding hydrochloride acid to alcohol (alcohol∶H 2O=1∶1) solution of the extract and Hydrastine (99.5%) was gained by CLC method with the yield 1%.

原药材用混合溶剂直接提取分离，可得到含北美黄连碱 97.7%的结晶体，提取率达 6 5%；提取物经醇水溶解、酸化，可直接获得小檗碱盐酸盐结晶，纯度达到 99.9%；用柱色谱法，可得到 99.5%的北美黄连碱，收率约 1

After determinating the iso-electric point of protein in brewer's spent grain, the protein could be extracted from brewer's spent grain by alcohol -alkali.

通过试验在确定了啤酒糟蛋白质的等电点后，采用醇-碱法提取啤酒糟中的蛋白质，得到了一种合理的提取工艺，使啤酒糟中蛋白质的提取率达 45 。48

The most effective method to study rheology of mafic granulite is to perform creep tests using natural gabbro as well as using hot pressed synthetic samples prepared from the major mineral components extracted from mafic granulite.

选择与基性麻粒岩中主要矿物成分接近的天然辉长岩，或者从天然基性麻粒岩中挑选出主要矿物，通过热压合成人工样品，进行高温流变实验是研究基性麻粒岩流变的最有效途径。

American Spots Removing Powder This product is produced by American Scientists, who extracted effective ingredients from some plants, such as grape seeds, pine tree barks, glossy gendarmes, blueberries, cowberries and other plants, and composed by the latest hi-tech biosynthesis.

中译英测试B 美国祛斑粉剂-本产品是美国科学家从葡萄籽、松树皮、灵芝、蓝莓、越橘等植物中提取有效成份，并用最新高科技生物技术合成，所提取的成份富含各种去斑因子，该产品可以辅助治疗各种色斑；黄褐斑、雀斑、老年斑、皮肤色素沉着、黑斑、日晒斑、暗斑，使你的肌肤恢复光滑和白晰。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。