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Objective To establish a PCR method for detection of Enterobacter sakazakii. Methods DNA was extracted by thermal denaturalization method and phenol-chloroform method respectively.

目的 建立阪崎肠杆菌的PCR检测方法方法用热裂解法和酚-氯仿法提取DNA。

Methods Collected 25 extracted teeth with shallow dentin caries in 5 age groups, each group 5 teeth.

方法收集5组不同年龄组含有牙本质浅层龋的离体牙，每组各5颗牙齿。

For example; one advertisement promotes an insect spray while showing the actor having teeth extracted at the dentist's office.

例如，一则促销害虫喷雾器的广告，展示的却是演员有着从牙医工作室提取的牙齿。

The available tunneling depth of channel carriers is dependent of frequency. Hence traps density in opposition to dielectric depth is extracted by charge pumping current and frequency.

通道电子能穿隧的深度会随著不同操作频率而改变，因此可由电荷帮浦电流对应到频率便能得知在闸极介电层中不同深度的缺陷密度。

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA；分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针，混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods The genome DNA was extracted from cultured strains of 7 common dermatophyte fungi species and part of clinical specimen.

方法以皮肤癣菌的基因组DNA为模板，采用一对半真菌通用引物（NS5、ITS1、ITS4）行半巢式PCR扩增rDNA上的ITS段。

A white crystalline insecticide that has low toxicity for mammals; is used in home gardens; extracted from the roots of derris and cube.

一种白色的晶体杀虫剂，对哺乳动物有轻量毒性，用于家庭花园，从毛鱼藤和鱼藤的根部提取。

And finally,the dominant characteristics are extracted which are composed of representative vectors of faces through analyzing the eigencurves using Fourier describer.

作为对人面特征的描述，最后利用傅里叶描绘子对特征曲线进行解析提取关键特征，得到人面的表征向量。

Based on such consideration, a strong texture description operator used in computer vision called local binary patterns is introduced. In this paper, the classic LBP method is enhanced from three aspects for facial expression recognition: image data, constructing feature way and the way of combining all extracted features.

基于以上几点考虑，本文引入了在图形学中具有很强描述纹理能力的局部二元模式(Local Binary Patterns，LBP)算法，改进了其中的三点对表情识别不利的因素：(1)将原图像经过小波包分解并在其它频率图像为零的情况下重构，得到了四幅与原图像大小相等的不同频率图像，在一定程度上增加了原图像的数据，改进了原算法数据量不足的问题。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失