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After repeating 5 times, the result shows that the genome DNA can be extracted from the fresh specimens by the methods ,but fail to be extracted from the preserved specimens with the same methods through electrophoresis.

经 5次重复实验,结果表明,用两种方法均能从新鲜标本中获得较完整的DNA ,且DNA得率较高;而对于无水乙醇保存的标本,提取出的DNA用琼脂糖凝胶电泳检测不出DNA条带,说明标本在保存过程中DNA可能发生了变化,致使提取出的DNA量太少或未分离出DNA 。

Result The background noise of bidirectional electrophoresis map of protein extracted by TCAJA method was greater, its clarity was poor, its horizontal striations were more and there were tinny spots with irregular shape appeared on it. The variegated spots had difference from the normal protein spots, they formed high coverage and shading on the other protein spots and affected the accurate expression of map information. The background noise of bidirectional electrophoresis map of protein extracted by Trizol method was little, its horizontal striations and non-protein impurities were less, there was no shading phenomenon strong spots and mere protein spots, so it provided abundant information for quantitative and qualitative analyses on the proteome from buffalo grass seeds, Compared with TCA/A precipitation.

结果]TCA/A法提取的蛋白质双向电泳图谱背景噪音大,清晰度差,横向条纹较多,图谱上呈现多个形状不规则的斑点,杂色与正常蛋白质点有区别,对其他蛋白质点构成高度及盖和遮进,影响图谱信息的准确表达;Trizol法所提取的蛋白质样品,其双向电泳图谱背景嗓音小,横向条纹少,非蛋白类杂质少,无斑点间的这进现象,蛋白质点数量增多,为野牛草种于蛋白质组定量、定性分析提供了丰富的信息。

The fir essential oil was extracted from fir root by steam distillation method, from which cedrol was extracted, and then, cedrol methyl ether was synthesized based on cedrol.

以杉木根为原料通过水蒸气蒸馏法提取杉木精油,从杉木根精油中精制提取柏木脑,再以柏木脑为原料合成甲基柏木醚。

The confidence interval method and the confidence region method for the error analysis of extracted parameters are studied. The algorithm implementation of confidence analysis based on the AD technique and OOP technique is presented, which can distinguish the redundancy and the correlation of extracted parameters.

研究了模型参数误差分析的置信区间法和置信区域法,提出了置信分析在AD技术及面向对象设计基础上的算法实现,可以很好地实现了对模型参数的冗余或相关的识别。

The extracted P-wave is reverse-time extrapolated and imaged with a P-velocity model using a finite difference solution of the scalar wave equation, the extracted S-wave is reverse-time extrapolated and imaged similarly, but with an S-velocity model, and converted S-wave data requires a polarity correction prior to migration to ensure constructive interference between data from adjacent source.

在模型中,每个网格点的纵、横波成像时间,为震源到该网格点的纵波初至时间。利用纵波速度模型和声波传播方程的有限差分解,对反射纵波进行逆时延拓和成像,利用横波速度模型和声波传播方程的有限差分解,对转换横波进行逆时延拓和成像,并在偏移前进行极性校正。

From the sea ice motion data extracted from the MODIS and AVHRR images, it is found that the sea ice motion in the Bohai Sea is effected not only by prevailing winds but also by topographic steering, ocean currents and internal ice stresses. The extracted sea ice drift is similar to observed ice drift, and can clearly show the sea ice drift in the Marginal Ice Zone especially. It can compensate for the limitation of the local or single station observation.

利用MODIS和NOAA/AVHRR资料获取的渤海冰速资料的综合分析显示:渤海海冰运动,除受盛行风控制外,还受到复杂的海岸地形、流和冰内应力的共同作用,所得到的大范围海冰运动规律和多年历史观测资料分析结果基本一致,并清楚地显示了冰边缘带海冰运动的特征,弥补了局地、单站海冰观测的局限性。

This pigment was extracted with 1%NaOH, then was extracted for 3 times with petroleum ether and ethyl acetate.

结果表明,板栗壳色素易溶于碱性水溶液,不溶或难溶于非极性溶剂。

There are two main types of Evening Primrose oil, one is extracted using chemical solvents, whilst the other is extracted by mechanically pressing the oil from the seeds.

主要有两种类型的月见草油,是使用化学溶剂提取,而另一种是提取石油机械紧迫的种子。

Were done and no positive reaction was found.5. The DNA of the young leaves of 132 Bt transgenic plants was extracted to do PCR Five positive inversion plants were obtained and the inversion rate was 3.8%.6. The DNA of fire positive inversion plants obtained in Dl generation was extracted and tested. Two stable positive inversion plants were obtained in D2 generations.7 The way of flourescent mirror flat was used to observe the pollen tube extension circumstance in the flower post after self-pollination.

检测了132株,没有发现阳性反应。5、对转入Bt基因的132株植株的幼叶提DNA,作PCR检测。D1代获得5株阳性转化植株,分别是90035的4株,85—593的1株,转化率为3.8%。6、将D1代获得5株阳性转化植株的种子在温箱中发芽,提取DNA检测,得到D2代稳定遗传的阳性转化植株2株。7、应用荧光镜制片法观察大豆自花授粉后花粉管在花柱中延伸情况。

A set of complex Morlet wavelet are applied on projection slice of each direction to decompose each projection into several frequency channels,the average and variance extracted are computed in each frequency channel,and then linear regression model is employed to computer realationship feature between frequency channels.1-D DFT is applied to features and the amplitudes of Fourier coefficient are selected as features,then the extracted features are rotation invariant.

该算法是将一定大小的图像进行二维傅里叶变换;其次在变换后的图像中央选择一个圆盘区域,并在方向[0°,180°]内进行等间隔角度频率抽样,实现方向分解,使用一组复Morlet小波对每个方向上的映射切片进行小波变换,从而实现多通道频率分解;在各个频率通道中计算均值和方差作为特征,并利用线性回归模型计算频率通道之间的关系特征;将特征沿方向进行一维傅里叶变换并取其幅值,从而得到旋转不变性特征。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。