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Total genomic DNA was extracted from four marine fishes (Verasper variegates, Cleisthenes herzensteini, Turbot Scophthalmus maximus, red seabream Pagrosomus major) using a proteinase K and phenol-chloroform procedure. Long mtDNA fragments were amplified by long and accurate PCR using primers designed based on the mtDNA sequenses of Thunnus thynnus thynnus, flounder Paralichthys olivaceus and other related fishes.

用蛋白酶k酚氯抽提法从圆斑星鲽、高眼蝶、大菱鲆和真鲷4种海水鱼的肌肉组织中提取基因组DNA，根据金枪鱼、牙鲆及相关鱼类的线粒体DNA序列保守片断设计出扩增引物，扩增从16SrRNA到ND4之间约9400bp的mtDNA长片段。

It has an advantage above other sugar alcohols- it is all natural, extracted from birch trees, sugar cane bagasse, and corn cobs/stalks.

它有一个好处，凌驾于其他糖醇，它是全天然，提取桦木树，甘蔗渣，玉米棒子/秸秆等。

Renner-Nantz said that the most exotic or unusual source of natural colours in DD Williamson's portfolio is carmine, which is extracted from the cochineal insect Dactylopius coccus found on particular cacti from the genus Opuntia.

公司首席执行官沃尔夫冈伦纳- nantz 说，最奇异或不寻常的来源，自然的颜色在房屋署副署长威廉姆森的投资组合是胭脂红，这是摘自胭脂虫的昆虫 dactylopius 球菌上发现，特别是从仙人掌属仙人掌。

Cocoa butter is extracted from the seed.

牙科医生将她的智牙拔出。

In the discrete wavelet transform domain, the watermark information is embedded by quantifying the coefficient of the transform domain andthe watermark can be extracted without the original audio.

在离散小波变换域水印嵌入算法中，本文给出了小波变换域系数均值量化的水印嵌入方案。

In the discrete cosine domain, the watermark is embedded by quantifying the coefficient of the dct domain, spectrum-spread is applied. the watermark can be extracted without the original audio.

在离散余弦变换域，采用扩频技术通过对变换域系数进行量化来嵌入水印信息，在水印的提取过程中不需要原始音频信号的参与。

Hi the discrete cosine domain, the watermark is embedded by quantifying the coefficient of the DCT domain and the quantification procession is given; the watermark can be extracted without the original audio.

3在离散余弦变换域，通过对变换域系数进行量化来嵌入水印信息，给出了量化方案；在水印的提取过程中不需要原始音频信号的参与。

In the discrete cosine domain, the watermark is embedded by quantifying the coefficient of the DCT domain and watermark can be extracted without the original audio.

在离散余弦变换域中如何嵌入数字水印，会具有较好的脆弱性和透明性。

Protoscoleces of cyst were recovered from the brain of sheep naturally infected with coenurus of Taenia multiceps and total RNA was extracted.T.multiceps thioredoxin peroxidase cDNA fragment was amplified by RT-PCR from the total RNA,ligated into pMD18-T vector and sequenced.

从自然感染的病羊脑内采集多头蚴原头节，提取总RNA，采用RT-PCR技术扩增多头带绦虫硫氧还蛋白过氧化物酶基因片段，PCR产物连接到pMD18-T载体，转化至大肠杆菌DH5α后测序，发现克隆到的TmTPx基因片段开放阅读框为453bp，编码150个氨基酸。

And after the diesel has been extracted, the coffee grounds can still be used for compost.

在燃料油被萃取出来之后，剩下的咖啡渣仍然可以用来作为肥料。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失