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In the mutant plant,a genecalled Lcyc is extensively methylated and thus not expressed--and thismethylated state is heritable by subsequent generations of toadflax plants.

在突变体植物中，一个叫Lcyc基因广泛甲基化，因此不能表达。这一甲基化状态在云兰属植物中是能遗传的。

This is old ground to an extent, and has been covered extensively by Tomkins, among others, but the fact remains that Rafa has missed out on many of his first-choice targets due to a lack of funds, had nothing to spend last summer (when player sales are taken into account) and should not be realistically expected to mount a title challenge with the fifth most expensive team in the league.

这算是老生常谈了，而且汤姆金斯等人也多次就此作出过辩论，由于资金的匮乏，贝尼特斯已经多次退而求其次，眼睁睁地看着很多自己的第一选择被别的球队拿更大额的支票挖走，而今年夏天球队更是实现了转会盈利，看看资产净值仅排名联赛第5的这支球队，要求他一直围绕着冠军发起冲击是否合理？

It will consume lots of CPU time and hard disk space of computer in calculating the ultimate torsional moment of a hull girder by using FEM, especially for the complex structure. The ultimate torsional moment of box thin-walled beam is analyzed by using finite element method. The influences of different characteristic parameters on the ultimate torsional strength are extensively studied.

在船体梁扭转极限承载能力的有限元计算中，对於比较大而且形状复杂的结构，需要采用足够多的单元数来模拟其真实的破坏模态，因而会耗费大量的CPU计算时间和硬盘空间，并且往往因为单元数太多而使数值计算变得不现实。

Awarded the Wheelwright Traveling Fellowship by Harvard in 1951, he traveled extensively in England, France, Italy and Greece.

荣获wheelwright行奖学金，由哈佛大学，在1951年，他赴广泛，在英国，法国，意大利和希腊。

The structure and genetic control of trichome morphogenesis of Arabidopsis have been extensively characterized, showing a number of structural and genetic similarities to cotton fibers.

研究与棉纤维发育相关基因的启动子元件及调节这些基因表达的转录因子有助于了解棉花纤维起始和发育相关的分子机制。

Cordycepin and Cordycepin triphosphate have been used extensively in the study of gene/mRNA transcription.

因此，虫草素及其三磷酸苷被大量用于基因/mRNA 转录的研究。

It obtains the various optical images of the sample's nanometer surface, transcended the diffraction limit of conventional optical microscope, by using a fine optical-fiber tip probing ultra-high frequency optical information on the sample surface. And it has extensively been applied on life science, chemistry and material science fields.

它利用细小的光学探针探测样品表面隐失波的超高频空间信息，超越经典衍射极限的限制，可对样品表面纳米尺度区域的各种光学信息进行扫描成像，在生命科学、化学及材料科学领域中都有着广泛而重要的应用。

Since then, the subject has been extensively explored and it is still under investigation

从那以后，该课题获得了广泛的探讨并且仍在研究中

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

With "Possessing what we still were unpossessed by," a partly sexual metaphor is extensively punned on.

拥有我们依旧尚未被宰制的领域」这一句部分带有性暗示的比喻广泛弥漫。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。