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We explored many factors which influenced high efficient transformation of large DNA, such as prepartion of BAC invector and high-molecular-weight DNA, partial digestion, ligation, electroporation,,and provided a basis of construction of the whole library.

摸索了BAC载体制备、高分子量DNA制备、酶切、连接和电击转化等一系列环节对高效转化产生大片段DNA的BAC的影响，为整个文库的构建奠定了基础。

G. speech perception and understanding. These stages are described and interpreted in terms of the findings from recorded corpus and questionnaires in education. As for speech production, students' speech motives are explored in detail. Speaker's speech intention is interpreted in connection with listener's reception of it. Findings are presented to show that, in the course of formulating speech plan, the previous sentences is often processed, the construction of sentence is a process of accumulative expansion of speech items through sequential or inversive appending or insertion, and the interaction between long-term and short-term speech plans often occurs.

本文运用录音语料、问卷调查材料具体分析了师生在言语生成、言语理解各阶段的特点和规律：在言语生成方面，本文探讨了学生的言语动机及其分类；结合言语意图的领会考察了言语意图；发现在制订言语计划过程中，常常出现对所听话语的处理加工，语句的建构是一个语项累加扩展过程，这种累加可以是顺序的，也可以是插序或倒序的，言语计划的制订还表现为预定计划与即时计划的相互作用；考察了言语计划执行过程中的一些现象，如制订计划与执行计划的交替、执行计划的中断等，并分析了产生这些现象的原因。

The involutionary hypothesis has not explored the needed economic theories very well and the problem of the production value per capita that has not been increased in the long run.

过密化假设对需要用到的经济学理论，对人均产值长期没有得到提高这个问题，都没有很好地进行研究。

An American team explored a temple which stands in an ancient city on the promontory of Asia Irenic.

一个美国考古队在阿伊亚。

A regular contributor to National Geographic and GEO Magazines, he has explored subjects ranging from the remotest stretches of Arabia's Empty Quarter to the unknown tree people of Irian Jaya.

他经常为美国国家地理杂志和展望，他探索的课题，从沙特阿拉伯的空季延伸至最边远的伊里安查亚不明树人不等。

This was one of the questions that a small group of IMD students explored when we chose the Bangladesh elective course, led by Professor Jean-Pierre Lehmann.

这是一小群IMD 学生曾经探讨过的问题之一。当时我们选修了让？

In this AACS-compatible JEF system, many attack points exist and can be explored to defend it. Furthermore, multiple attack points can form multi-point collusion attacks, which also engage the proposed system.

在本技术中，我们探讨了许多可能的攻击点以及抵抗措施，此外，我们提出了多重攻击点所产生的串谋攻击以及合适的抵抗对策。

This thesis explored the interaction and combination course and reason between rites and penalty law at the juristic basis, the legislation and the judicative practice level in Chinese ancient history.

本文试图从礼刑互动关系的新的视角，来进一步探索中国古代礼与刑在法理思想、法律规范和司法实践三个层面的关系。

Finally, the relationships between physic stimuli and psychological nature of kinesthesis sensation was explored in the study. DIVAD110、DIVAD120、DIVAD1130、DIVAD140、of DIVAD system made in Germany were adopted as the main method to explore the kinesthesis sensation of waist and neck.

该实验首先确定动觉的心理属性包括肌紧张感与运动幅度感，并采用客观指标——复制力与角度的误差绝对值大小，分别作为反映它们高低的指标，在综合这两个指标的基础上考察不同水平自由跤运动员动觉感受性的差异。

Objective To seek new activated killer cells, the feasibility of preparing LAK cell from umbilical blood was explored.

目的 寻找新的高效杀伤活性的细胞，探讨脐血单个核细胞作为LAK细胞前体细胞的可行性和意义。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。