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The discussions which the study investigated were chicken and pork meat processing for elders via different tender mode. In this study, we want to evaluate the nutritions of elders and focus on animal protein. The experiment was injected marination with CaCl2, NaHCO3 and enzyme on pork and chicken meat quality traits. Treatment on the meat was analyzed proximent quality and textural properties by response surface stepwise regression. The best tender method was discovered the elder's sensory evaluation of different tender mode and observed degradation of myoglobulin by muscle tissue slice and SDS-PAGE (10%). The function of sous-vide cooking collocated with tenderization that was evaluated by textural properties and sensory evaluation. It looks for discussion about meat processing for elders.

中文摘要本试验目的在利用市售禽畜肉为原料，针对高龄者之营养需求，分别使用氯化钙、碳酸氢钠与嫩化酵素等常见之嫩化方式对禽畜肉进行嫩化处理，搭配反应曲面法分析一般特性与物性测定，并进行高龄者品评测试以探讨不同嫩化方式之口感影响以求得不同禽畜肉之最佳嫩化处理，并以肌纤维组织切片与电泳分析观察肌肉蛋白质之分解现象，最后利用真空调理法搭配嫩化处理并分析物性测定与品评测试，评估真空调理法增进嫩化之效果，探讨禽畜肉开发为适合高龄者之加工产物模式。

Besides, The nodulation with else host experiment told us that NWGX035 could nodulate with melilotus suaveolens Ledeb, sophora alopecuroides Linn, lotus corniculatus Linn, sophora viciifolia Hance, trifolium repens Linn. The property of powerful salt, alkali, high temperature endurance and wide nodulation could confirm this strain was a particular strain.

另外，回接其它寄主植物的试验表明， NWGX035能与草木樨、黄化苦豆子、百脉根、狼牙刺和白三叶共生结瘤，其广泛的共生结瘤范围和强的耐盐、碱和抗(来源：A9fBC论文网www.abclunwen.com)高温特性可以证明该菌株为一株性状优良的菌株，其共生结瘤机制和耐盐碱机制需进一步研究。

Recombinatino polypeptide or chemically synthesized polypeptide antigen is used to immunize the mammal to obtain polyclonal antibody and the nonconservative amion acid sequence of the antiboby to N-terminal with recognition site qBrn-2 of development regulatory protein qBrn-2. The antibody does not recognize other POU structure domain protein, and recognizes specifically qunique protein in Western imprinting experiment. The qBrn-2 expression mode obtained immunohistochemistry process is identical with the resulted height of in-situ molecular hydridization.

以重组多肽或化学合成多肽为抗原免疫哺乳动物获得的多克隆抗体，这一抗体对发育调控蛋白qBrn－2的识别位点为qBrn－2的N－末端的非保守性的氨基酸序列，该抗体不识别其它POU结构域蛋白；在Western印记实验中，该抗体特异地识别单一的蛋白；利用免疫组织化学接示的qBrn－2表达模式与分子原位杂交的结果高度一致。

With the nymphae of L. imitate on longan tree as tested objects, the 98.5% N24 mineral oil pesticide and 99% EnSpray mineral oil EC were diluted with water at 100 and 200 times of solution resp., the control effect of 98.5% N24 mineral oil pesticide on L. imitate of longan was researched by field experiment.

方法］以龙眼树上的白蛾蜡蝉若虫为供试对象，用98.5%N24矿物油杀虫剂和99%绿颖矿物油乳油分别对水稀释100和200倍液，通过田间试验，研究了98.5%N24矿物油杀虫剂对龙眼白蛾蜡蝉防治效果。

With vertical-section analysis, in the later of experiment, naked terra and wheat land make content of soluble salt high in 0～20cm,and decreasing with deepening of soil-layer; the content value of planting two-year-old Onobrychis viciaefolia 、one-year-old Onobrychis viciaefolia 、interplanting and sequential cropping in the every soil-layer is equal; in harvest, only planting wheat and interplanting display content of soluble salt is high in middle-layer (20～60cm) and low in surface and down layers(0～20cm,60～80cm).

土壤垂直剖面分析，试验末期裸地的土壤盐分表现为0～20cm 土层高，随土层深入含量逐渐降低；二年生红豆草、一年生红豆草、套种和复种处理的土壤盐分均表现出各层含量近似相等的特征；小麦收获期，单作小麦和小麦套种地各土层的可溶性盐含量均表现为中层(20～60cm)高、上下层(0～20cm 和60～80cm)低的特征。

This experiment was conducted with swamp buffalo to compare the effects of different acquisition methods, serum-free medium or serum-contained medium and serum-contained medium with many growth factors, on the culture of oviductal epithelial cells.

以广西沼泽型水牛输卵管为材料，比较研究不同取材方法、有无血清及生长因子对水牛输卵管上皮细胞培养的影响。

Methods DCs were prepared from peripherad blood mononuclear induce d with gra-nulocyt e/macrophage colony-stimulating factor and interleukin-4. Apoptosis of glioma cells were induced with γ-radiation. We design these experiment groups including (1) coculture of DCs and apoptotic glioma cells and T cells,(2) coculture of DCs and U937 cells and T cells,(3) coculture of DCs and cultured glioma cell and T cells,(4) coculture of Des and T cell.

用粒－巨噬细胞集落刺激因子加白介素－4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人脑胶质瘤细胞凋亡，将DCs、T淋巴细胞和凋亡胶质瘤细胞共培养，同时设计不同类型肿瘤细胞（U937及培养胶质瘤细胞）作对照，分离、富集DCs、T淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

Methods DCs were pre pared from peripherad blood mononuclear induce d with gra-nulocyt e／macrophage colony-stimulating factor and interleukin-4.Apoptosis of he patochlangioma cells were induced with γ-radiation.We design these experiment groups including(1)coculture of DCs and apoptotic cancer cells and T cells,(2)co culture of DCs and necrosis cancer cells and T cells,(3)coculture of DCs and cul tured cancer cell and T cells,(4)cocultu re of DCs and T cell,(5)cocuture of DCs and cultured cancer cell.

用粒-巨噬细胞集落刺激因子加白介素-4(IL-4)从人外周血分化、诱导DCs、γ-射线在体外诱导培养的人胆管癌细胞凋亡，将DCs、T淋巴细胞和凋亡胆管癌细胞共培养，同时设计不同类型肿瘤细胞(坏死胆管癌细胞及培养胆管癌细胞)作对照，7d后，分离、富集DCs、T 淋巴细胞进行免疫应答及肿瘤细胞杀伤试验。

The experiment obtains the noon peroxy free radical density approximately 11 pptv, the observation had discovered the free radical the density has the direct ratio with the ozonedensity but to have the inverse ratio with the nitrogen dioxide density.

实验测得中午过氧自由基的浓度约11pptv，观测发现自由基的浓度同臭氧浓度成正比而与二氧化氮浓度成反比。

Phellodendron amurense Rupr. stem segment with axillary bud and top bud were cultured with MS and 1/2MS medium in this experiment,in order to study the effects on rapid propagation of P. amurense in different culture conditions.

摘要以黄檗带顶芽和腋芽的茎段为材料，以MS和1/2MS为基本培养基，进行黄檗组培苗的快速繁殖，以研究不同培养条件对黄檗快速繁殖的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。