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Changdong" showed a noticeable enhancive effect when it was co-administrated with other anti-tumor agents or when it was co-applied with radiotherapy. The action mechanism study showed that an apparent cytotoxic effect of "Changdong" on ten tumor strains in vitro with a quantity-effect relationship was observed. Immunity experiment showed that "Changdong" could induce proliferation of spleen lymphocyte in mice bearing tumor and could improve NK cell" s activity, whereas has no impact on IL-2" s reproduction. It was also noticed that "Changdong" could induce human liver cancer QGY" s apoptosis in vitro, and it happened mainly on G0-G1 phase.

结果:"长动"对小鼠移植瘤模型和人体肿瘤裸鼠异种移植均有明显抑制作用,量效关系明显:"长动"与放疗和化疗分别联合应用有明显的增效作用:作用机制研究结果:细胞毒性试验研究表明"长动"在体外对10株肿瘤细胞有明显的细胞毒性作用,量效关系明显;免疫学试验表明"长动"可以诱导荷瘤小鼠的脾淋巴细胞增殖,增强NK细胞的活性,对IL-2的产生无明显影响;"长动"在体外可明显诱导人体肝癌QGY细胞凋亡,使细胞阻滞于G0-G1期。

Part three: An experiment study on the repair of peripheral nerve injury by NT-3 gene modified neural stem cells objective To investigate the effective treatment of peripheral nerve injury with NT-3 gene modified neural stem cells, to explore the feasibility of the treatment by means of gene transfection Methods 54 SD rats were divided evenly into 3 groups. The sciatic nerve transected model were made and sutured end to end with epineurium. The neural stem cell without NT-3 gene or with NT-3 gene modified were injected into triceps muscle one time a week, saline injected as control group. The nerve trunks of stomas and triceps muscle were acted as specimen three weeks、six weeks and nine weeks after operation.

最后,为了探讨神经营养素-3基因修饰的神经干细胞对周围神经再生修复的影响,从转基因角度探讨治疗周围神经损伤的有效方法,我们选用54只SD大鼠随机分为3组,造成坐骨神经切断损伤模型,神经外膜端端对位缝合后,于小腿三头肌每周分别注射生理盐水、未被神经营养素-3基因修饰的神经干细胞、及神经营养素-3基因修饰的神经干细胞。

SLNs of 48 patients with early-staging breast cancer were detected by methylene blue staining SLNB with intraoperative frozen section combined with rapid immunohistochemical staining by EPOS. 28 patients were found negative, and were randomized to receive simplified ALND(n=15, experiment group) or standard ALND(n=13, control group).

30例早期乳腺癌行美蓝染色SLNB术中行SLN冰冻切片联合EPOS法快速免疫组化检查,其中阴性者28例分成两组,15例行简化的ALND(仅清扫胸小肌外侧组淋巴结,简化组),13例行标准ALND。

And the last, the effects of fermented cottonseed-hull on dairy performance were studied with feed tests. In experiment 1, Cottonseed-hull and comminuted cottonseed-hull were fermented with complexing strains of microbials including EM and probiotics, using seven pairs of formulation have been formed by corn meal, molasses and fecula combined with urea and zein meal.

试验一利用EM菌液与益生素混合扩大培养后的复合菌液,选用玉米粉、糖蜜、淀粉三种炭源和尿素、玉米蛋白粉两种氮源共组成了7组发酵底物配方,将棉籽壳分成微粉样与原始样,发酵结束后对测定结果进行统计分析。

Using solution culture experiment, discussed the influnce of nitrobenzene to the germinationrates and seedling growth of ten leguminous crops, and results showed that, when the nitrobenzeneconcentration was 1~100mg·L~(-1), it had little influnce to germination rates of leguminous crops,with the increasing of nitrobenzene concentration, emergence rates of leguminous crops decreased,but no significance compared with control, and found that the toxicity of nitrobenzene toleguminous related with the concentration, the thresholds of nitrobenzene toxicity to differentleguminous crops were different, under the test conditions, the inhibition of root was larger thanhypocotyl, according to the relationship between the inhition rates of root and hypocotyl length andthe nitrobenzene concentration, the sensitive order of the ten leguminous crops to nitrobenzenewas:small-seedsoybean>kidneybean>Dongnong42>brownbean>Dongnong49>Dongnong46>ormosia>french bean>black bean>mung bean.

本研究探索了硝基苯对十种豆类作物的发芽率及幼苗生长的影响,结果表明:硝基苯浓度为1~100mg·L~(-1)时,对豆类作物发芽率影响不大,随着硝基苯浓度的增加,豆类作物出苗率有所降低,但与对照差异不显著,研究发现硝基苯对豆类作物的生物学毒性同浓度有关,硝基苯对不同的豆类作物的毒害阈值不同。

Field experiment was carried out to study the effects of KEON and PEON (organic N extracted with phosphate buffer) from 4 kinds of organic wastes on the growth and N use efficiency of green soybean. The results show that the application of PM could apparently improve the shoot dry weight, seed protein content and the yield by 20.4%, 17.8% and 38.0%, respectively, compared with regular chemical fertilizer applied. The application of OMM could significantly improve the shoot dry weight, seed protein content and the yield by 33.2%, 16.2% and 32.5%, respectively, compared with regular chemical fertilizer applied.

通过田间试验研究4种有机废弃物中的KEON和PEON2种形态的有机氮对菜用毛豆生长及化肥氮利用率的影响结果表明:施用鸡粪和豆粕混合肥与单施化肥相比,地上部分干物质重分别提高20.4%和33.2%,籽粒中的蛋白质含量分别提高17.8%和16.2%,达到显著差异水平;与单施化肥相比,毛豆产量分别提高38.0%和32.5%。

After comparing the image effect of homomorphic filtering, the effect of median filtering with different templates and the effect of binarizing with improved OTSU, the experiment results show that V groove laser image in underwater welding is preprocessed very well through such serial processing steps as homomorphic filter strenghening, median filtering, binarizing with improved OTSU, and thinning.

通过比较同态滤波前后效果、比较不同模板下的中值滤波效果、以及Otsu法改进前后的效果,实验表明,对水下焊接V型焊接坡口激光图片依次进行同态滤波增强、中值滤波、改进Otsu二值化和腐蚀细化一系列图像处理,取得了效果较理想的预处理结果。

The protocol for the detections of all the AIV isolates, the H5-subtype and the optimization of reactions were studied with a H5-subtype reference isolate BSG1. A 229bp-band of AIV specific and a 380bp-band of H5 subtype specific were amplified individually with the designed primers of AIV specific and H5-subtype specific respectively by the developed RT-PCR technique from isolate BSG1. A double-PCR was also developed which can detect all the AIVs and identify the H5-subtype AIVs. The results of specificity experiment showed that there was no specific band could be amplified with the samples of Newcastle disease virus, infectious bronchitis virus, infectious laryngotracheitis virus, egg drop syndrome virus and infectious bursal disease virus.

结果应用通用引物可从BSG1毒株扩增到与预计大小相符的229bp AIV特异性片段,应用H5亚型鉴定引物则可从BSG1毒株扩增到与预计大小相符的380bp H5亚型特异性片段;在此基础上建立了可检测所有AIV毒株以及可同时进行H5亚型毒株鉴定的二重RT-PCR方法;检测方法的特异性试验结果表明,该方法对新城疫病毒、传染性支气管炎病毒、传染性喉气管炎病毒、产蛋下降综合症、传染性法氏囊病毒无交叉反应;检测方法的敏感性试验结果表明,通用引物扩增、H5亚型特异性引物扩增以及二重PCR扩增反应的组织样品总RNA的最低检出量分别为0.0217μg、0.0217μg和2.17μg;对20份临床样品检测中,有10份为AIV阳性,其中有5份为H5亚型病毒阳性。

We also found that the content of Na(superscript +), K(superscript +) and proline in leaves varied with different treated days. Under all treatments, the absorption of Na(superscript +) increased and the content of proline in leaves decreased with the prolonging of treated days. The absorption of K(superscript +) measured on the 7th day and the 21st day increased and was restrained on the 14th day, which lead to the decline of K(superscript +)/Na(superscript +) ratio. While the content of praline measured on the 14th day remarkably increased with the increase of salinity. Our experiment indicated the live oak seems to adapt the salt stress by increasing the absorption of K(superscript +) during the earliest and late stages of salt stress and accumulating proline in leaves during the middle stage of salt stress.

同时发现,盐胁迫处理不同时期对叶片脯氨酸含量和Na、K含量的影响各不相同,在处理7d和21d时,叶片对Na和K的吸收量均有增加,使K/Na维持相对稳定的比值,而脯氨酸含量随NaCl质量质量浓度的增加减少;在处理第14d时,对Na吸收增加的同时,抑制了对K的吸收,导致K/Na比值随NaCl质量质量浓度的增加而迅速下降,而此时叶片脯氨酸含量随着盐质量质量浓度的增加而迅速增加,表明在盐胁迫早期和晚期,弗栎可能通过增加对K的吸收以减轻Na离子毒害效应,而在盐胁迫中期,叶片积累脯氨酸是适应盐胁迫的方式之。

The relation of interfacial adsorption equilibrium in the solution system was respectively tested and calculated with Gibbs equation and dynamic method .The conclusion showed that (1) the experiment result of dynamic method with was highly in coordination with the value which from theoretical calculation.(2) The surfactant molecule was in arrangement of monomolecular layer at the gas-liquid interface.

用吊环法测定了十二烷基硫酸钠水溶液不同浓度与其界面张力的关系,拟合得到的回归方程为σ=-0.9523 ln ~2C-24.534 ln C-81.411,同时还测得其临界胶束浓度值CMC为6.5×10~(-3M,运用吉布斯等温方程和动态法分别计算和测定了该溶液系统的界面吸附平衡关系,结论表明:(1)动态法测定结果基本吻合吉布斯方程的理论计算;(2)表面活性剂分子在气—液两相界面是以单分子层形式定向有序排列的。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。