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Methods: Foty healthy New Zealand white rabbits were randomly divided into four groops. Control groop was fed with normal diet for 10 weeks, model groop was fed with 1.5% cholesterol diet plus normal diet; APS groop was fed with 1.5% cholesterol plus 12.0mg/kg APS plus normal diet; Captopril groop was fed with 1.5% cholesterol plus 15.0mg/kg Captopril plus normal diet. After ten weeks' experiment, the concentration of each group's Triglyceride, Total Cholesterol, High Density Lipoprotein Cholesterol, C Reactive Protein, Nitric Oxide, Maleic Dialdehyde and Endothelin-1 were measured, so was the activity of superoxide dismutase.
将40只雄性新西兰兔随机分为4组,每组10只,对照组餵普通饲料;模型组:普通饲料加质量分数为1.5%的胆固醇餵养;黄芪多糖组:普通饲料加质量分数为1.5%的胆固醇餵养,同时腹腔注射APS(12.0mg/kg);卡托普利组:普通饲料加质量分数为1.5%的胆固醇餵养,同时灌喂卡托普利(15.0mg/kg);10周后,测定各组空腹血清或血浆三脂酰甘油、总胆固醇、高密度脂蛋白胆固醇、一氧化氮、内皮素-1(ET-1)、丙二醛、C反应蛋白含量以及超氧化物歧化酶活性,并计算主动脉内膜粥样斑块面积。
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Methods HAb18Fab was prepared by papain digesting HAb18, and it was radioiodinated with iodine-131 using high efficiency iodination method. The experiment consisted of two parts: ten nud mice bearing human hepatocellular carcinoma xenograft were injected intravenously with 131Ⅰ-HAb18Fab 3 700kBq/mouse, and six patients with hepatocellular carcinoma were injected intravenously with 131Ⅰ-HAb18Fab 111-166.5 mBq/body.
以木瓜蛋白酶切割肝癌单克隆抗体HAb18制备HAb18 Fab,应用高效碘标记法将131Ⅰ标记于HAb18 Fab,再以131Ⅰ-HAb18 Fab偶合物进行荷肝癌裸鼠及肝癌病人体内放射免疫显像。
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Methods: In acute or chronic experiment, all SD rats were randomly divided into three groups which included microthromboembolism group with the injection of 400 μg sodium laurate into the coronary arteries of SD rats, TS group with drinking telmisartan (10 mg/kg), and NS group with NS instead of drinking telmisartan (10 mg/kg), and NS group with NS instead of sodium laurate.
将SPF级SD大鼠冠状动脉注射月桂酸钠400μg后,随机分入微栓组和替米沙坦组(饮水中给药10mgkg^(-1)d^(-1)),对照组则以等量生理盐水替代月桂酸钠。
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Finally, simulation software was developed with Visual C++ and Delphi for 2D molding analysis of MIM, which affords facilities for the quality forecast of 2D thin wall parts and optimal design of mold and process parameters. In the research of molding performance of MIM feedstock, for the shortcoming of capillary rheometry, it was propounded that using spiral mold test molding performance of feedstock, and a set of spiral mold was creatively designed with quickly exchange gates and closed mold cavity. With the spiral mold, an orthogonal experiment was made on getting regress equation of flow length L〓 of the heavy alloy feedstock. Then the rule of L〓 varying with Q〓, P〓, and ΔT〓 was discovered. Q〓 ranks first in the influence on process parameters of molding performance, P〓 and Δ T〓 second. Therefore the injection rate Q〓 should be controlled primarily to improve the flow performance of feedstock.
在MIM喂料注射成形性能研究方面,针对毛细管流变仪测试法衡量流动性能的不足,提出使用螺旋线模具测试喂料熔体的注射成形性能,创造性地设计了一套可快换浇口,模腔封闭的螺旋测试模,并在螺旋测试模上采用正交实验设计,得出了高密度重合金喂料流动长度L〓的回归公式,揭示了L〓随Q〓、P〓、△T〓和S〓的变化规律,并得出工艺参数对成形性能影响的强度顺序为Q〓最大,P〓与△T〓其次,因而改善喂料流动性能应优先控制注射速率Q〓。
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It is necessary to carry out a thorough study on pest control technology with DMDS, with an expectation of obtaining a new fumigant of methyl bromide alternative.2. Investigation of the toxicity of fumigation on adult and mixed stages of the three most tolerant insect species -- Sitophilus oryzae, Rhyzopertha dominica and Tribolium castaneum -- under a range of EtF、DMS and DMDS doses in a Flow-through Fumigation System shows that the FTFS procedure offers a constant concentration of fumigant with low CO2 concentrations and ensures that the fumigant toxicity is effectively not compromised by CO2. In comparison with chamber fumigation method, the toxicity data of EtF、DMS and DMDS obtained from this experiment is more accurate by using FTFS.
主要研究结果如下:1、甲酸乙酯和二甲基二硫对仓储害虫具有良好的控制作用,应加快甲酸乙酯替代溴甲烷防治仓储害虫开发应用,同时,二甲基二硫是很有潜力的仓储害虫防治熏蒸剂,要深入进行二甲基二硫控制有害生物的技术研究,以期获得新的溴甲烷替代熏蒸剂。2、利用循环熏蒸系统测定了甲酸乙酯、二甲基二硫和甲硫醚对米象Sitophilus oryzaeL。
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The studies was proceeded on the the supercritical CO2 extraction technique of rotenone from Tephrosia vogelii. The extraction influences of technology conditions were systematically groped, The research results were listed as follows:1 The analysis method applied in this paper has been proved to be suitable to technology research of the studies on the SC-CO: extraction technique of rotenone from Tephrosia vogelii. 3 indexes, extraction ratio of rotenone. extraction ratio of total compounds and rotenone content percent in total compounds weight, were carried out with HPLC quantitatively determination combined with the sample weight loss after extraction. With these 3 indexes. The extraction influences of technology conditions were evaluated by synthesis. Then the best technology conditions were ascertained for different extraction aims.2 The pretreatment of rough material should be needed before extraction. Comminuting the material to 0.28mm size is most fit to SC-CO: extraction.3 Firstly static extracting 20 min then turn to dynamic doing is the better extraction mode than other ones.4 The orthogonal experiment was conduct with 3 factors and 5 levers.
本论文通过对非洲山毛豆SC-CO_2萃取中影响萃取效果的工艺条件的研究,初步得出如下结果:(1)本论文中所采用的萃取效果评价方法适宜于非洲山毛豆中鱼藤酮的超临界流体萃取技术研究,该方法具体为:HPLC定量分析非洲山毛豆SC-CO_2萃取物中的鱼藤酮含量,再结合萃取前后处理样品的重量损失,得出鱼藤酮萃取率、萃取所得粗物质萃取率和粗物质中鱼藤酮含量三个参数,以此三个指标综合评价萃取条件对萃取效果的影响;(2)通过对原料粒度对萃取效果影响的研究,结果表明SC-CO_2萃取非洲山毛豆复叶中的鱼藤酮,原料粉碎成30目粒度叶粉时萃取效果较好;(3)通过对萃取方式与萃取效果之间关系的研究,结果表明SC-CO_2萃取非洲山毛豆中鱼藤酮应采用先静态后动态的萃取方式,静态萃取时间为20min;(4)通过温度、压力和CO_2流量三因素五水平的正交试验,结果表明温度是最主要的影响因素,压力因素对鱼藤酮萃取率的影响程度高于CO_2流量,而对于粗物质萃取率和粗物质中鱼藤酮含量CO_2流量因素高于压力。
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Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.
第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml,分离单个核细胞体外诱导培养DC,并将实验组和对照组细胞各分为三组,A组为未处理组,B组为冻融组,C组为RNA转染组。A组DC在培养5d后,每孔加入TNF-α200U/ml;B组DC培养第5d后,每孔中加入癌细胞蛋白抗原,使DC/肿瘤细胞=1:1,继续培养4h后,每孔加入TNF-α200U/ml;C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC:RNA=1×105/ml: 5ug,按照invitrogen DMRIE-C试剂说明书进行操作),转染结束后用完全培基取代转染剂,每孔加入TNF-α200U/ml。
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Compare the lung injury extent and the important physiological parameters to determine the optimal condition for rabbit ventilator induced lung injury, Choosing another 24 new Zealand rabbit, randomly divided into 4 groups:(1)conventional ventilation+PEEP group;(2)ventilator induced lung injury group: employ the experimental condition determined by the first part experiment;(3)VILI+HO-1 inducer hemin group:pre-treated with HO-1 inducer, the ventilation method is the same with VILI group;(4)VILI+HO-1 inhibitor Zn-PP group(Zn-PP group):pre-treated with HO-1 inhibitor, the ventilation condition is the same with VILI group.
第二部分24只家兔随机分为4组:(1)常规潮气量+PEEP组;(2)呼吸机所致肺损伤组:使用实验第一部分确定的最适条件;(3)呼吸机所致肺损伤+HO-1诱导剂干预组:预先给予氯铁血红素,通气条件同VILI组;(4)呼吸机所致肺损伤+HO-1阻断剂干预组:预先给予锌原卟啉,通气条件同VILI组。
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Methods: The interference evaluation experiment proves that IVIG exerts strong inhibitions in Bacterial Endotorxin Test. While the interfering factors can't be eliminated by treatments such as dilution and neutralisation, they are liable to be inhibited provided that the IVIG samples are to be diluted by 1:4 with the Dilution Solution Ⅰ.The BET for IVIG preparation diluted by 1:4 with the Dilution Solution Ⅰ is carried out comply with the Chinese Pharmacopoeia(2000 Edition) The lysate with sensitivity of 0.125Eu/ml is used in the test,and the results of the BET are comparable to that of Pyrogen Test carried out in rabbits.
通过干扰评价实验证明IVIG对细菌内毒素检查法有强烈的抑制作用,单纯用简单稀释和调整pH值法无法消除干扰,如果用稀释剂对IVIG作1:4以上的稀释,即可消除样品对该检查法的干扰,利用灵敏度为0.125EU/ml的鲎试剂,将样品用稀释剂稀释4倍,按中国药典(2000版)进行细菌内毒素的检查,结果与家兔热原质试验进行比较。
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A testing triangulation experiment for a polygon with 100 inside islets was done with this algorithm, and the result showed that this algorithm is efficient and robust for triangulating complex polygons with large numbers of inside islets. This algorithm has been successfully applied to the three- dimensional ore-body modeling and visualization system, in which it is used to triangulate the sectional polygons with inside islets, e.g., the inside stones or holes in ore-body, and its high efficiency has been proved.
实验表明,该算法在含有大量岛屿的带岛屿多边形三角剖分中具有很高的时间效率和很强的鲁棒性,并成功将其应用到基于剖面的三维矿体建模与可视化系统中,解决了含有夹石或孔洞的矿体剖面多边形三角剖分问题,具有一定的实际应用价值。
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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.
曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。
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The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.
稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。
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When I was in school, the rabbi explained everythingin the Bible two different ways.
当我上学的时候,老师解释《圣经》用两种不同的方法。