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And it was divided into four groups at randomly (n=6),The vertical compression,bending and torsion intensity in without and with reduction of cortical fragment of plate fixated contra side, without and with reduction of cortical fragment of plate fixated sidewere measured.A11 results were analyzed with statistic method.2、animal experiment :The 12 adult goats were divided into two group at randomly,wedged cortical defect with 1 cm of long and conjugate diameter was created in the middle-part of the medial femur and plate was fixed in the defect side and as the experimental side,the other side was control.after operation 4、8、12 weeks,investigation of the fracture healing by means of gross observation,X-ray examination and histology in both sides.

测试各组的垂直压缩、三点弯曲及抗扭转的刚度。2、动物实验：12只健康成年山羊，雌雄不限，体重在20～25kg之间，在同一只羊双侧股骨中段内侧制造楔形骨皮质缺损，分实验组和对照组两组进行钢板内固定并植骨，于术后4、8、12周对实验组及对照组行X线正位片检查，观察骨折愈合过程中不同时间点的影像学特点。于4、8、12周各组处死4只动物行大体标本观察，并取缺损区骨组织行组织学检查。X线检查及组织学检查结果根据LaneX线评分标准及组织学评分标准进行评分。最后采用SPSS13.0进行统计学分析。

This paper presented the research and exploration of mechanism and technics as well as the design of the magnetic circuit of this new compound machining, based on above, establishing the parameter model, meantime, analyzing influential effects on productivity of magnetic and EDM compound machining the holes through large numbers of experiments, including velcoity contrast experiments of pre-compound and after-compound machining with the change of current, velcoity contrast experiments of pre-compound and after-compound machining with the change of magnetic induction, velcoity contrast experiments of pre-compound and after-compound machining with the change of impulse width, experiments of the superficial roughness, experiments of the Electrode wastage, and intersectant experiments analysis as well as the change experiments of current waveforms. Through a number of experiments we analysed the influential effects of magnetic and EDM compound machining, compared the factors with EDM by oneself. A large of experiment records and deducibility have demonstrated that the productivity of magnetic and EDM compound machining is developed by 20%～400% than traditional EDM.

本论文对磁场电火花复合加工这种新的复合加工的机理、工艺和复合加工的磁路设计进行了研究和探索，在此基础上，建立了磁场电火花复合加工的参数模型，同时通过大量的随着电流变化磁场电火花复合前后加工速度对比实验、磁感应强度变化时的加工速度变化实验、随脉冲宽度变化的加工速度实验、表面粗糙度实验、电极损耗量实验、磁场电火花复合加工正交实验分析、电流波形变化等实验，实验分析了影响磁场电火花小孔加工效率的因素，并且跟单一的电火花加工中的影响因数进行了比较，大量的实验数据和理论推导证明磁场电火花复合加工比传统的单一电火花加工的效率提高了20％～400％左右。

The vapour and plasma ignition times of the target are got based on the equation of heat conduction and cascade model. We suppose that the plasma is ignited when 210 generations of new electrons are born in vapour generation time. The influence of temperature on the thermodynamics and optics parameters of material also have been considered in the model. The resulting theory has good agreement with the experiment and overseas report. A blade method to measure the laser spot is given in this paper with validation and error analysis. The plasma threshold of metals in atmosphere and water ambients both are diagnosed with the light deflection and piezoelectric transducer. A Q-switched pulsed Nd:YAG laser operating at infrared (1064nm), visible(532nm) and ultraviolet (355nm) wavelengths has been used. Al、Fe、Cu are used as targets and get a similar results with both method. Theoretical and experimental analyses are applied on the influence of wavelength on the threshold have been done, both of which have shown that the plasma threshold of metals decrease as the laser wavelength increases; The plasma threshold of metals are higher in water than in air and the pressure of the shock wave in water is five times higher than in air.

从热传导方程和雪崩电离机制出发，假设当电子增值2~(10)倍时，考虑了温度对材料热力学和光学参数的影响时，得到了气化和等离子体点燃的时间，利用该模型进行计算得到的结果与国内外报道及自行通过实验测得的阈值基本一致；提出了利用刀刃法测量激光光斑面积的方法，并通过实验进行了验证和误差分析；利用光偏转装置和压电换能器分别对空气和水中金属等离子体点燃阈值进行了实验诊断，激光器均为调Q—YAG激光器(波长1064nm，532nm，355nm，脉宽10ns)，靶材分别为Al、Fe和Cu，两种测试方法得到的等离子体点燃阈值基本一致；本文从实验和理论计算两个方面讨论了波长对等离子体点燃阈值的影响，均得到了等离子体点燃阈值随着波长的增加而减小的结论；对空气中和水中不同环境下金属等离子体的点燃阈值进行了比较研究，得到了金属在水中的等离子体点燃阈值比空气中的大，且水中产生的冲击波的压强是空气中的5倍左右的结论。

The method provides preparation with metal pattern the candle of same size 96, the false tooth that 2 kinds of brands use after breaking candle base hold colophony in the palm to fill glue, undertake with 4 kinds of methods heat treatment makes its solidify, detect with machine of all-purpose and mechanical experiment base hold resinous bending intensity in the palm, mix with double element variance analysis multiple undertake statistical processing quite.

方法用金属模具制备相同尺寸的蜡型96个，失蜡后使用2种品牌的义齿基托树脂填胶，用4种方法进行热处理使其固化，用万能力学实验机检测基托树脂的挠曲强度，用双因素方差分析和多重比较进行统计学处理。

The result also showed that return treatment is better than taken treatment within different treatments. 4. According to the experiment when the plant was harvested(In March 25th,2009), it was found that the rape with NPK treatment,especially with NPK-T treatment in Quaternary red soil and in granitization red soil, grew abnormality or dead in the centre of district(80%),which yield was extremely low,but had some to the remaining(20%) close to the cement ridge.Among the three types of parent material,eighteen districts,Dischaemum Schreb of gramineae plants could only grow in granitization red soil with NPK treatment,and the other sixteen districts were little or even not.The rape planted in Quaternary red soil with NPK treatment got disease especially in the centre of the district.

其次是M处理，CK最低。3、三种母质种植油菜均表现为：归还处理好于带走处理；4、据试验收产时（2009年3月25日）发现：四纪红土和花岗岩二种母质的NPK处理的油菜除靠近水泥梗边行长有部分油菜外（占小区面积20%），小区中间（占小区面积80%）的油菜生长不正常或死亡，产量极低；三种母质共18个小区，只有花岗岩母质的NPK处理长有禾本科植物马唐草，其余16个小区很少或没有；四纪红土NPK处理油菜病害严重。

In general, the design work with traditional measures is done by hand, which cannot meet the needs of the technology and manufacture. So the author has developed a CAD hydraulic design software fit for lower specific speed axial flow runner, which is easily operated by users.The main research work of this paper is as follows:1 Analyzing the main reason of axial flow power station cannot work well under littler volume of flow in some season and putting forward an improving scheme, pointing out the characters of design under abnormal factors.2 Combined with the development of the industry in our country, the abnormal hydraulic design method of propeller turbine is discussed based on present literature, data and experiment results.3 Guided by the design method of software engineering, a suit of hydraulic design software of Lower Specific Speed Axial Flow Runner is programmed by the object-oriented programming language Visual Basic and a straightforward, concise and standard Windows human-computer dialogue interface is designed for the system, which makes the operation of the system very convenient.4 ActiveX Automation technology is adopted as the interface with AutoCAD and the hydraulic drawings are edited, modified by AutoCAD until hydraulic model drawings according with the demand of engineering practice are output.5 To resolve practice problem, the author has designed a lower specific speed axial flow runner for Duowa Power Station through an example.

论文的主要内容有：1 深入分析很多轴流定桨式水电站枯水季节小流量下不能发电的主要原因，提出减容改造方案，并指出超常规条件下的改造设计特点。2 结合国情及行业发展需要，参考现有资料、文献和前人的研究成果，进行低比转速轴流式转轮叶片水力设计方法的研究，对轴流式转轮叶片的奇点分布法水力设计进行了全面的探索，结合现代计算机技术，找到了一种针对特殊条件的轴流式转轮叶片水力设计方法。3 仔细比较多种AutoCAD二次开发工具后，结合本课题的特点，选用Visual Basic语言，综合应用软件工程的设计思想，进行低比转速轴流式转轮叶片水力设计软件的编制，为系统设计了直观、简洁和标准的Windows人机对话界面，便于用户操作；编程时在考虑到设计条件特殊性的同时，尽量做到程序的通用性，使之可用于解决同一类问题；另外，大部分程序还可移植到普通轴流式转轮叶片水力设计中，具有良好的适应性。4 利用ActiveX Automation技术与AutoCAD接口，对AutoCAD进行二次开发，在AutoCAD下实现水力图的自动生成和编辑，直至输出符合实际工程要求的木模图。5 通过算例，为多哇水电站设计了一个低比转速轴流定桨式转轮，解决其在枯水期小流量下不能发电的问题。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

To evaluate the efficacy of the recombinant fowlpox virus vaccine against the heterotype IBV, the chickens were challenged with the homotype IBV LX4 strain and the heterotype IBV LTJ95I strain after three weeks post vaccination. Antibodies against IBV were detected in vaccinated chickens one week post inoculation. The CD4+ T-lymphocytes in the peripheral blood increased rapidly in all groups challenged with IBV, except for the vaccinated group challenged by heterotype strain and the low level of CD4+ T-lymphocytes remained until end of the experiment. In all the groups, a high level of CD8+ T-lymphocytes only was observed in the vaccinated group after challenging with IBVhomotype strain. The morbidity and the mortality of this group were 21.43 % and 0 %, respectively, which showed significant difference with other groups. In addition, the lesions of chickens and virus shedding were less in the vaccinated group challenged by IBV homotype strain comparing with other groups, but there was no difference for the average body weight of chickens in all groups.

结果显示，重组疫苗接种1周后，免疫鸡产生抗IBV的抗体；而且外周血中CD4+和CD8+ T淋巴细胞的含量略高于非免疫对照组；攻毒后，异源强毒株攻毒的免疫组CD4+ T淋巴细胞呈下降趋势，并且该组低水平CD4+的状态一直持续到试验结束，而其他组CD4+ T淋巴细胞均迅速上升，峰值达到14.5 %；同源强毒株攻毒的免疫组CD8+ T淋巴细胞呈高水平的表达，而其他组攻毒后均无明显变化；保护率结果显示，同源强毒株攻毒免疫组的发病率和死亡率为21.43 %和0 %，与其他各组相比均有显著差异；另外同源强毒株攻毒的免疫组病理损伤与异源强毒株攻毒的试验组相比明显减轻，其排毒时间和排毒量也均有所减少；强毒攻毒后所有试验组体重无显著差异。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件，消融人肝细胞癌株SMMC-7721细胞悬液，分为五组：A、对照组，B、45℃10min组，C、50℃3min组，D、54℃3min组，E、60℃即刻组，消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤，间隔1周，右侧腹股沟皮下接种瘤块作为观察瘤，成瘤后分为四组：A组，对照组，不行治疗；B组，微波组，仅微波消融小鼠消融瘤；C组，成熟DC组，微波消融小鼠消融瘤，并于小鼠双侧腋下注射肿瘤抗原致敏的树突状细胞；D组，未成熟DC组，微波消融小鼠消融瘤，并于消融瘤周注射未成熟树突状细胞。

The desulfurization experiments of CaO samples with various fractal dimensionswere carried out in the TGA to investigate the influences of fractality on the sulfationreaction, and the evolution of pore structures during the reaction. Under certainsulfation conditions, the calcium conversions of the CaO samples increase with theincrease in fractal dimension when fractal dimension is less than 2.68, and decreaseswhen it is more than 2.68. In the range of the experiment temperatures, the calciumconversion for a sample with a lower fractal dimension increases with the increase inthe temperature, and for a sample with a higher fractal dimension there exist anoptimum temperature at which the sample can reach the highest calcium conversion.

选用具有不同分形结构的CaO试样进行了硫化实验，研究孔结构的分形特性对硫化反应的影响以及硫化过程中分形孔结构的变化特性，实验结果表明，在一定的分形维数范围内，随着分形维数的增加，钙利用率增大，但分形维数超过一定值时(约为2.680)，钙利用率反而降低。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。