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The fragment was connected to pcDNA3.0 carrier which was cut by the same two enzymes to form the plasmid which was transformed into Escherichia coli DH5a.Appropriate clone was cultured to extract plasmid.The extract was analysed to confirm that it was constructed correctively.

将该片段连接到同样被KpnⅠ和XhoⅠ双酶切的pcDNA3.0载体中，转化大肠杆菌DH5α感受态细菌，挑取阳性克隆进行质粒提取，进行酶切鉴定，并获得阳性克隆。

Methods: Based on the results analyzed correlatively form the SGRQ and the examination of function, 46 patients with COPD are divided into the control group and the therapeutic group.

目的：应用呼吸问卷评价慢性阻塞性肺疾病患者生活质量的价值。

The result of experiments show that resynthesized speech signals form the its correlogram by auditory model inversion is nature and robust in noisy environment.

实验结果表明，我们通过听觉模型反演从信号的自相关图谱中恢复出的语音信号，具有较好的自然度和良好的噪声鲁棒性。

To discuss this wave aspect, it is useful to invoke a form of the correspondence principle.

为讨论这个波动性质，利用对应原理的一种形式是有好处的。

It extends the correspondence principle to the physical picture of microscopic system and the existance form of the space,thus confirms the truthfulness and the objectivity of microscopic particle.

将"对应原理"推广于微观体系的物理图象及空间存在形式，肯定了微观粒子的真实性和客观性。

Each of you will receive a copy of the other's form so that you can begin corresponding.

你们每个人都会收到其他人的表格的副本，这样你就可以开始通信了。

In these cased, human-leaded culture is just for format, not for practice; and to solve these problems needs the corroborative work form government, enterprises and employees.

这些问题的解决有待于政府、企业和员工的共同努力。

The rubber cover, which is made form chemical resistant materiral. Has fine anti-chemical corrosiveness and good physical properties.

选用耐酸碱的材料加工制成覆盖蹭，具有良好的抗化学腐蚀性能和良好的物理性能。

They come in the form of corruption and poverty.

他们来，在形式的贪污和贫穷。

Postcards,locks of hair,and the dried corsage form their senior pro

明星片，留作纪念的头发和高中毕业班会上得到的已经干枯的花束

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。