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Hu said he will meet with Japan's Emperor Akihito, have an in-depth exchange of views with Japanese Prime Minister Yasuo Fukuda on bilateral relations and other issues of common concern, and have extensive contact with Japanese people from different walks of life.

胡锦涛表示，他将会见明仁天皇陛下，同福田康夫首相就中日关系和共同关心的问题深入交换意见，广泛接触日本各界人士。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

To adapt from the natural and humane environment, to inherit and keep and comply with with admitting with continuity, local culture respect and carry on with development in spirit traditional moral character.in the beginning of the new century , the renew of the education system, the transition of the thought mode, and the development of science andtechnology impels universitys architecture cultural developement in the 21st century from sealed to open socialization, and we must pay close attention to people and the nature ecologinically,pay more attention to the informationization that knowledge exchange, and combine the intelligent trend of the technical development.

新世纪刚刚开始，教育体系的更新、思维模式的转变、科学技术的发展促使21世纪的高校建筑文化发展由封闭到开放的社会化、关注人与自然的生态化、注重知识交流的信息化、结合技术发展的智能化趋势。

Two of these protein bands, with a molecular weight of 12 000 Mr and 14 000 Mr, showed immunoreactivity with IgE in the sera from patients with allergy to pollen of Caryota ochlandra. For the pollen extract of Carvota mitis, 30 protein bands were detected in SDS-PAGE, and three of these protein bands, with a molecular weight of 12000, 14000, and 26000 Mr, showed immunoreactivity with IgE in the sera from patients with pollen allergy of Carvota mitis. The 14000 Mr protein was the major allergen. Result from the ion-exchange chromatography indicated that the two pollen allergens (12 000、14000 Mr) from Caryota ochlandra were eluted in the second peak. The major pollen allergen of Carvota mitis was eluted in the forth and fifth peak.

结果 长穗鱼尾葵花粉有30余条蛋白带，其中主要条带有18条，12000和14000 Mr为长穗鱼尾葵花粉特异性变应原；短穗鱼尾葵花粉有30余条蛋白带，其中主要条带有9条，26000、1200和14000 Mr为短穗鱼尾葵花粉特异性变应原，其中14000 Mr为主要变应原；通过离子交换层析方法纯化出长穗鱼尾葵花粉相对分子质量为12000和14000的变应原主要分布在Ⅱ峰中，短穗鱼尾葵花粉相对分子质量为14000阳的主要变应原分布在Ⅴ峰和Ⅵ峰中。

Three kinds of BCRC No.51534, 10322 and 10675 would be selected and acted as an experimental sample of Escherichia coli. Results shows that Escherichia coli of No.51534 will appear better performance because the maximum of open circuit voltage, closed current and power density are 1.01V, 22mA and 1342mW/m2, respectively. Concerning the effect of culture time with respect to different phase type on the electricity performance of MFCs, the time points on the intersection between lag phase and logarithmic phase, the middle of point of stationary phase for growth curve of Escherichia coli would appear a good performance of MFCs. In addition, the BCRC No. 51534 Escherichia coli possessing a better performance of MFCs than others would be suggested and applied to further studying. Comparison with the performance of MFCs with respect to electron mediator under different mole number, result shows that electron mediator of methylene blue with 4.63mM would appear a better electricity performance of MFCs than others. Concerning the different material of proton exchange membrane with PTFE-Nafion, Nafion 211, 212 and 117 with respect to the performance of MFCs, result shows that the Nafion 117 applied in MFCs will have a better performance of MFCs than other cases. Finally, the effect of molar concentration on the performance of MFCs would be expected at the studied cases of 0.4M, 0.2M, 0.1M and 0.05M respectively for cathode oxidant, result shows that a good performance of MFCs will happen at the condition of 0.2M. Those observations will be useful to improvement of MFCs in the further study.

於上述电池系统条件下，进行大肠杆菌生长曲线、电子传递介质、质子交换膜、电极与阴极氧化剂对电池电性效能分析；选择编号10322、10675与51534之大肠杆菌为实验菌株，依定量培养之生长曲线取出代表不同时生长特性时期的培养时间，利用亚甲基蓝作为电子传递介质进行实验分析从所测得的电量进行分析，以编号51534之大肠杆菌的微生物燃料电池有最大的开路电压为1.01V及最大闭路电流为22mA；当极化曲线中电压为0.47V、电流为11.4 mA时有最大的功率密度为1342 mW/m2；加以负载有平均工作功率密度294 mW/m2；从生长曲线与电性效能来分析，得知生长曲线的迟滞期与对数期的转变点与静止期的中间点有最佳电性效能表现；对於加入不同莫耳数之电子传递介质methylene blue、neutral red与thionine之电池效能表现，则以加入4.63mM methylene blue电子传递介质的电池有较佳平均功率密度230 mW/m2；另对於质子交换膜PTFE-Nafion、Nafion 211、Nafion 212与Nafion 117之电池效能表现，以Nafion 117质子交换膜的电池有较佳平均功率密度340 mW/m2；对於分析加入不同莫耳数浓度0.4M、0.2M、0.1M与0.05M的阴极氧化剂之电池效能，则以0.2M的阴极氧化剂的电池可得到较佳平均功率密度429 mW/m2。

With a background of heated gold investment, most new passionate investors came into the market without simply understanding the gold investment. This made many investors lost their capital when first stepped into the market. In addition, many gold exchange types are specialized in enlarging guarantee sum. Compared with investment in stock market, investors who invested in types characterized in enlarging guarantee sum would likely to face more risks and challenges. Those risks could enlarge investors' one-year loss in stock market to one-day loss in gold market. Nowadays, Chinese gold market can be differentiated into material gold market, paper gold market, in-stock gold and future gold market. Because of the enlarging system in international gold market, investors may lose to the bottom in one day if he or she chose a guarantee sum dealer with an highly enlarging system. Of course, this is an ultimate example. In gold market, the type with the least risk is material gold, such as gold memorative silver coin, material piece of gold and gold brick. Material gold investment enjoyed its popularity among middle or large cities located in the middle and eastern part of China.

在黄金投资火热的背后，是大部分新入市投资者盲目的热情，由于对黄金投资缺乏了解，很多投资者入市即被套，而且很多黄金交易品种具备保证金放大效应，和在股市投资相比，投资者炒作保证金放大品种进行投资，往往需要面对高额风险，这种风险可以使投资者在股市中一年时间遭受的损失在一天内就提现出来，现在国内黄金市场主要分为实物黄金市场、纸黄金市场、黄金现货、黄金期货市场，其中国际黄金现货市场由于存在高度放大机制，如果投资者选择具备高度放大的保证金交易商，经常会出现一天内就会血本无归的情况，当然，这是极端的情况，黄金市场中，风险最小的是实物黄金，例如黄金纪念金银币、实物金条、金砖，实物黄金投资在中东部地区大中城市比较流行。

With a background of heated gold investment, most new passionate investors came into the market without simply understanding the gold investment. This made many investors lost their capital when first stepped into the market. In addition, many gold exchange types are specialized in enlarging guarantee sum. Compared with investment in stock market, investors who invested in types characterized in enlarging guarantee sum would likely to face more risks and challenges. Those risks could enlarge investors' one-year loss in stock market to one-day loss in gold market. Nowadays, Chinese gold market can be differentiated into material gold market, paper gold market, in-stock gold and future gold market. Because of the enlarging system in international gold market, investors may lose to the bottom in one day if he or she chose a guarantee sum dealer with an highly enlarging system. Of course, this is an ultimate example. In gold market, the type with the least risk is material gold, such as gold memorative silver coin, material piece of gold and gold brick. Material gold investment enjoyed its popularity among middle or large cities located in the middle and eastern part of China.

在黄金投资火热的背后，是大部分新入市投资者盲目的热情，由于对黄金投资缺乏了解，很多投资者入市即被套，而且很多黄金交易品种具备保证金放大效应，和在股市投资相比，投资者炒作保证金放大品种进行投资，往往需要面对高额风险，这种风险可以使投资者在股市中一时间遭受的损失在一天内就提现出来，现在国内黄金市场主要分为实物黄金市场、纸黄金市场、黄金现货、黄金期货市场，其中国际黄金现货市场由于存在高度放大机制，如果投资者选择具备高度放大的保证金交易商，经常会出现一天内就会血本无归的情况，当然，这是极端的情况，黄金市场中，风险最小的是实物黄金，例如黄金纪念金银币、实物金条、金砖，实物黄金投资在中东部地区大中城市比较流行。

With a background of heated gold investment, most new passionate investors came into the market without simply understanding the gold investment. This made many investors lost their capital when first stepped into the market. In addition, many gold exchange types are specialized in enlarging guarantee sum. Compared with investment in stock market, investors who invested in types characterized in enlarging guarantee sum would likely to face more risks and challenges. Those risks could enlarge investors' one-year loss in stock market to one-day loss in gold market. Nowadays, Chinese gold market can be differentiated into material gold market, paper gold market, in-stock gold and future gold market. Because of the enlarging system in international gold market, investors may lose to the bottom in one day if he or she chose a guarantee sum dealer with an highly enlarging system. Of course, this is an ultimate example. In gold market, the type with the least risk is material gold, such as gold memorative silver coin, material piece of gold and gold brick. Material gold investment enjoyed its popularity among middle or large cities located in the middle and eastern part ofChina.

在黄金投资火热的背后，是大部分新入市投资者盲目的热情，由于对黄金投资缺乏了解，很多投资者入市即被套，而且很多黄金交易品种具备保证金放大效应，和在股市投资相比，投资者炒作保证金放大品种进行投资，往往需要面对高额风险，这种风险可以使投资者在股市中一年时间遭受的损失在一天内就提现出来，现在国内黄金市场主要分为实物黄金市场、纸黄金市场、黄金现货、黄金期货市场，其中国际黄金现货市场由于存在高度放大机制，如果投资者选择具备高度放大的保证金交易商，经常会出现一天内就会血本无归的情况，当然，这是极端的情况，黄金市场中，风险最小的是实物黄金，例如黄金纪念金银币、实物金条、金砖，实物黄金投资在中东部地区大中城市比较流行。

Finally, simulation software was developed with Visual C++ and Delphi for 2D molding analysis of MIM, which affords facilities for the quality forecast of 2D thin wall parts and optimal design of mold and process parameters. In the research of molding performance of MIM feedstock, for the shortcoming of capillary rheometry, it was propounded that using spiral mold test molding performance of feedstock, and a set of spiral mold was creatively designed with quickly exchange gates and closed mold cavity. With the spiral mold, an orthogonal experiment was made on getting regress equation of flow length L〓 of the heavy alloy feedstock. Then the rule of L〓 varying with Q〓, P〓, and ΔT〓 was discovered. Q〓 ranks first in the influence on process parameters of molding performance, P〓 and Δ T〓 second. Therefore the injection rate Q〓 should be controlled primarily to improve the flow performance of feedstock.

在MIM喂料注射成形性能研究方面，针对毛细管流变仪测试法衡量流动性能的不足，提出使用螺旋线模具测试喂料熔体的注射成形性能，创造性地设计了一套可快换浇口，模腔封闭的螺旋测试模，并在螺旋测试模上采用正交实验设计，得出了高密度重合金喂料流动长度L〓的回归公式，揭示了L〓随Q〓、P〓、△T〓和S〓的变化规律，并得出工艺参数对成形性能影响的强度顺序为Q〓最大，P〓与△T〓其次，因而改善喂料流动性能应优先控制注射速率Q〓。

From May 4th Movement of 1919 to the 1920s, sex love narrations in Chinese novels were rather an enlightenment and liberation in that particular era with four characteristics. Firstly, sexual desire was endowed with rationality with regard to liberation of human nature and mutual love. Secondly, with an epochal concern for a stronger and superior race, greater attention was paid to sexual parties concerned, mainly physically. Thirdly, new ethics on sex were promoted in that personal freedom on sex love was emphasized and sex love became an important way of self-fulfillment. Therefore, independence of sex love was advocated and the scope of sex love was also widened. Fourthly, the sex consciousness of this period had the mixed features of the past and the contemporary. Sex love narrations in the 1930s and 1940s showed a further exploration on the theme of human nature. First of all, sex was restored to a kind of desire. ShenCongwen probed the importance of harmonious sex love to a sound personality and Shi Zhecunrevealed that sexual inhibition could lead to the distortion, even frenzy of human nature. Bothexplored the influence and functions of sexual desire as human nature upon human personality,giving prominence to the irresistibleness of sexual desire and the significance of sexual desire asa motive. Then, human bodies driven by sexual desire were described and given greater attentionto. Next, ethics on sex were deconstructed either by Shen Congwen who opposed man-madeethics with law of nature and the School of New Sensation who replaced traditional ethics on sexwith exchange rules in consumption, or by Shi Zhecun who spared no efforts in representing thecomplete failure of social rules, including ethics on sex, due to the impact from sexual desire.The direct effect was a paleness of the ethical binding force on sex. Last, sex consciousness inthe works of female writers emerged. In the early works of Ding Ling, the sexual desire of thefemale is no longer a ramification of emotion but an independent one. In addition, in the sex lovenarrations of other female writers, such as Mei Niang, strong self-consciousness on the socialstatus of the female is also reflected. Sex love narrations in the late 1970s and early 1980s wereactually a re-enlightenment in the spirit of humanism, whose major features include humanisticlegitimation of sex love within the boundary of ethics, a blend of sex love narrations withnational concepts and national myths as well as the aesthetic, poetic descriptions of humanbodies etc. In the middle and late 1980s, sex love narrations entered a stage of exploration forpluralism. First, by restoration of sexual desire, writers of this era restored human nature, thevery original state of human being. Different emphasis and tendencies could be seen in differentwriters: looking at sex love dialectically and trying to describe its creative as well as destructivecharacteristics; connecting sex love with violence and trying to reveal the evil side of humannature through it; emphasizing the creativeness of sex love especially in the heritage of"wildness".

从&五四&到20世纪20年代，中国小说的性爱叙事是一种处在时代夹缝中的启蒙和解放，其所表现出来的特点，一是正面赋予性欲望以合理性，这种合理性是在人性解放和爱情前提下获得的；二是在强种育种的时代焦虑影响下小说性爱叙事表现出对物质身体的关注，其焦点主要在体格方面；三是树立起新的性伦理道德观念，表现为强调在性爱问题上的个人选择自由，性爱成为个人自我实现的重要方式，从而赋予人在性爱问题上的独立性，同时还扩大了性爱表现的领域；四，这个时期小说性爱叙事中所表现出的性意识还具有新旧杂糅的特点。30、40年代小说性爱叙事表现出对人性主题的深化与丌拓，首先是把性还原为欲望本身来探索，沈从文从和谐性爱对健全人性的决定作用、施蛰存从性压抑会导致人性扭曲甚至疯狂的角度探讨作为人类本质性欲望的性对人性产生的影响和作用，突出了性欲力量的不可抗拒和作为人类行为的重要动力等方面的内容；其次在叙事中表现出对欲望化了的身体的描绘与关注；第三是解构了性的伦理道德，无论是沈从文以自然法则来反对人为的道德设置还是新感觉派用消费交换原则替代传统性伦理道德，或是施蛰存极力表现包括性伦理规范在内的社会法则在性欲力量冲击下溃不成军，其直接的效果都是使性伦理道德对人的约束力量显得苍白虚弱；第四，出现了由女性作家表现的女性性意识，在丁玲的早期作品中女性的性欲望不再是作为感情的衍生物而是独立的欲望存在，而在梅娘等其他女作家的小说性爱叙事中则表现出对女性社会地位的强烈自觉意识。70年代末80年代初期小说的性爱叙事实际上是在人道主义语境下的再次启蒙，其主要特征一是性爱在人道主义前提下获得合法性，但要求性爱不超出伦理规定的范围；再就是将性爱叙事同国家观念和民族神话相融合；此外还有对身体的描写审美化诗意化等。80年代中后期，小说性爱叙事进入了多元化探索的阶段，作家通过还原性欲望来还原人性、还原人类的本真状态，不同作家笔下表现为不同的侧重和倾向——以辨证的方式看待性爱，写出其具有的创造性力量和毁灭性特征；将性爱与暴力相联系并通过其来表现人性的黑暗；强调性爱的创造性作用，尤其在民族&血性&的传承方面。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。