查询词典 eukaryotic

Aim To construct anti-HFRS virus human engineering antibody expression vectors and then to express it in eukaryotic cells.

目的构建人源性抗HFRS病毒基因工程抗体基因的表达载体，为其进一步在真核细胞中表达奠定基础。

Objective To express human phospholipid hydroperoxide glutathione peroxidase in eukaryotic cells and to prepare its polyclonal antibody.

目的在真核细胞中表达人磷脂氢谷胱甘肽过氧化物酶并制备抗PHGPx多克隆抗体。

The mechanism of action performed by the interaction between Cap and CBP-eIF4E in the initiation of mRNA-directed tra nslation and the role of another newly-found CBP complex played in intranuclear mRNA processing are discussed.

该文主要讨论了帽结构与胞质中的CBP-eIF4E（eukaryotic initiation factor 4E，真核起始因子4E）的相互作用在mRNA指导的翻译起始中的作用机制，以及帽结构与核内发现的另一种CBP复合体相互作用在mRNA加工中的作用。

The cells were transfected with the eukaryotic expression vector αS1-LA-psv and the activity of β-galactosidase was detected after transfected from 24 h to 120 h. Human alpha lactalbumin was detected at 72 h after transfected too.

将构建的真核表达载体αS1-LA-psv 转染奶牛乳腺上皮细胞，培养24～120 h均检测到了β-半乳糖苷酶的表达；培养72 h检测到细胞中人α-乳白蛋白的表达，表达量约为

Objective Consequently, by constructing the pcDNA3-HA-hdacl, a HDAC1 eukaryotic expression vector, we were to study the interaction between HDAC1 and ERa and ERB in vitro and the effects of HDAC1 on the ERa and ERB at the protein level. So we can partly reveal the multiplication, differentiation and cancerization of lacteal gland cells.

目的 本文意在通过构建可在真核细胞中表达的重组质粒pcDNA3-HA-hdacl，研究组蛋白去乙酰化酶1(HDAC1)和雌激素受体ERα和ERβ在体外的相互结合作用，以及HDAC1对ERα和ERβ蛋白水平的影响规律，从而在一定角度揭示乳腺细胞的增殖、分化和癌变的过程，为更好地寻找治疗癌症的药物靶标打下基础。

Methods Regulation region of bovine β-lactoglobulin geneand full-length genomic DNA encoding hEPO were used to construct eukaryotic expression vector,and the transgenic goats were produced by pronuclear microinjection.

方法采用牛β-乳球蛋白基因调控元件和hEPO全长编码序列基因组DNA构建真核表达载体，应用受精卵原核注射的方法制备hEPO转基因山羊。

Nuclear lamina is a ubiquitous structure in eukaryotic cells.

核纤层是真核细胞普遍具有的结构。

The genes gB,gC and gD amplified from infectious laryngotracheitis virus Chinese Wanggang strain were cloned into the Eco RⅠ site of the eukaryotic expression vector pCAGGS,re-spectively. The positive recombinant plasmids were screened by transforming into E.coli DH5 α cells,which were named pCAGG-gB,pCAGG-gC and pCAGG-gD respectively after identification by restriction endonuclease analysis and sequencing analysis.

将传染性喉气管炎病毒王岗株的gB、gC、gD基因分别克隆到真核表达载体pCAGGS的 Eco RⅠ位点，经过酶切、测序分析，筛选鉴定出含有gB、gC、gD基因的重组质粒，分别命名为pCAGG-gB、pCAGG-gC、pCAGG-gD。

AIM: To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani anddetectexpressionofthegeneinNIH3T3cells.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

METHODS: Amastin gene was amplified from nuclear DNA of Leishmania Donovani isolates and cloned into an eukaryotic expression vector pcDNA31.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。