查询词典 esterase

The substrate specificity test showed that the lipolytic enzyme from strain DF-B6 was an esterase, and not a lipase.

底物特异性实验确定DF-B6菌株分泌的脂裂解酶为酯酶，而非脂肪酶。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

Malassezia can breakdown lipid like esterase,and result in morphological changes of malpighian cell and cell apoptosis,these are all their main etiological factors.

马拉色菌能像酯酶一样分解脂质，而且可引起角质形成细胞形态学改变和细胞凋亡，这些都是它的主要致病因素。

Low methoxyl pectin was obtained from fresh orange peel by using Na2CO3 as an activator to active pectin esterase in the raw material and pectin was de-esterified.

以新鲜橙皮为原料，在盐酸水解乙醇沉淀提取果胶之前，激活并利用果皮中固有的果胶酯酶进行果胶的酶法脱酯，制备低甲氧基果胶，以产品的甲氧基含量和果胶得率为指标，确定最佳工艺条件。

Molecular systematics of partial species belonging to 5 subfamilies of Miridae are investigated in this study by using esterase is...

运用酯酶同工酶和线粒体DNA中编码细胞色素B蛋白质的基因Cyt b作为分子标记，对盲蝽科Miridae的5个亚科的部分种类进行了分子系统学研究。

Molecular systematics of partial species belonging to 5 subfamilies of Miridae are investigated in this study by using esterase isozyme and cytochrome b gene in mitochondrial DNA as molecular marks.

运用酯酶同工酶和线粒体DNA中编码细胞色素B蛋白质的基因Cyt b作为分子标记，对盲蝽科Miridae的5个亚科的部分种类进行了分子系统学研究。

The following examining methods were carried out:(1)Phagocytosis of Monilia albicans by the peritoneal macrophages by means of fluorescence microscopy after acridine orange staining.(2)Measurement of non-specific esterase in Mφ after being stained with α-nephthy acetate by image analysis.

激光照射结束后取小鼠腹腔巨噬细胞，分别做如下实验：(1)用荧光显微镜观察腹腔巨噬细胞对白色念珠菌的吞噬功能；(2)用α-荼酚醋酸酯法显示腹腔巨噬细胞内非特异性酯酶，并用图像分析系统对NSE作定量分析；(3)用透射电镜观察腹腔巨噬细胞超微结构的变化。

The Esterase, Catalase, PPO isoenzyme and soluble mycoprotein patterns of 43 isolates were analyzed by polyacrylamide gel electrophoresis.

利用聚丙烯酰胺凝胶电泳技术对43个新月弯孢分离物进行了酯酶等三种同工酶及可溶性蛋白的分析。

Pathogenieity and virulence of the isolates were assessed; Soluble mycoprotein and esterase etc isoenzynie patterns of 43 isolates were analyzed; Genetic diversity among 40 isolates of Curvularia lunata was evaluated by random amplified polymorphic DNA fingerprint.

通过对43个新月弯孢分离物的3种同工酶及可溶性蛋白分析和40个分离物的RAPD指纹图谱分析，表明玉米弯孢菌叶斑病病原群体存在遗传多样性。

Some are slowly progressive and a myopathic EMG may occur if there is an associated endplate myopathy (eg, in slow channel syndrome or acetylcholine esterase deficiency) or in DOK7 synaptopathy.

一部分缓慢进展，当伴随有终板的改变（如：在慢通道综合症或胆碱酯酶缺乏）或者在DOK7突触病时，EMG呈肌源性改变。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。