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Episode 22:Legacy(2007.05.09)●Of all the preposterous assumptions of humanity,nothing exceeds the criticisms made of the habits of the poor by the well-housed,well-warmed,and well-fed.

Herman Melville【赫尔曼·梅尔维尔(美国作家、诗人，代表作『白鲸』，1819-1891)：人性中所有荒谬的傲慢里，没什么能超过来自拥有豪宅、温热和美食的人对穷人的指责。

Episode 22: Legacy（2007.05.09）●Of all the preposterous assumptions of humanity, nothing exceeds the criticisms made of the habits of the poor by the well-housed, well-warmed, and well-fed.

梅尔维尔（美国作家、诗人，代表作『白鲸』，1819-1891）：人性中所有荒谬的傲慢里，没什么能超过来自拥有豪宅、温暖和美食的人对穷人的指责。

◎Episode 22: Legacy（2007.05.09）●Of all the preposterous assumptions of humanity, nothing exceeds the critici sms made of the habits of the poor by the well-housed, well-warmed, and well-f ed.

Herman Melville 【赫尔曼·梅尔维尔（美国作家、诗人，代表作『白鲸』，1819-1891）：人性中所有荒谬的傲慢里，没什么能超过来自拥有豪宅、温暖和美食的人对穷人的指责。

I watch the twenty-sixth episode of the History of the World as soon as I get home this afternoon.

今天下午一回到家中，我便看了《世界历史》的第二十六集。

I watch the twenty-fourth episode of the series teleplay World History first this morning.

今天早上，我看了电视系列剧《世界历史的第二十四集》。

Often appearing vividly was also an image of myself, in a black turtle-neck pullover and a red woolen skirt, head wrapped in a red silk scarf, walking along the ginkgo tree lined streets on a windy autumn day, toward my first job after university…Years have passed, and I still feel like a bird, eager to fly. The allure is the flying itself, not to any destination but to fly. Absence of a goal creates freedom, while every episode along the way lingers in the memories.

连自己那时的样子，也跃然眼前，秋天起风的日子里，常常是一条红格呢裙子，黑色高领羊毛衫，头上包着一块红丝巾，穿过种满银杏树的巷子，去打大学毕业后的第一份工，这么多年过去了，我好像还是一只想飞的鸟，那召唤来自飞翔的感觉，不为到哪儿，只为飞。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区，克隆进PET-32a表达载体，双酶切和PCR鉴定出阳性者，命名为PET-32pgE，转化BL21菌株，挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和，按2％体积转种另一管，培养至OD_(600)为0.6，加IPTG至终浓度1mmol／l，诱导4～6小时，SDS-PAGE和Westernblot分析，证明确实表达并具有免疫反应性。

Whether this is the Chinese jewellery local or foreign businessmen brought in, and it is an excellent witness to foreign trade, maritime Silk Road is a section of episode.

不管这珠宝是中国本地或是外国商人带进来的，它都是中外贸易往来的极好见证，是海上丝绸之路的一段插曲。

Butters discovers his dad at a gay bath house (Episode 514): Butters was designed to be the complete opposite of Cartman in every way -- naive, sweet-natured and idiotic.

Butters发现他老爹出入同志浴室（第五季14集）：在剧中Butters天真、善良、又冒着点傻气，与Cartman毫无共通之处，是一个和Cartman截然不同的角色设定。

Butters discovers his dad at a gay bath house (Episode 514): Butterswas designed to be the complete opposite of Cartman in every way --naive, sweet-natured and idiotic.

Butters发现他老爹出入同志浴室（第五季14集）：在剧中Butters天真、善良、又冒着点傻气，与Cartman毫无共通之处，是一个和Cartman截然不同的角色设定。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。