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In this heating step the hydrolytic enzymes are inactivated and the wort can be pitched inoculated with the desired yeast.

在加热过程中，水解酶丧失了活性，而且我们可以接种酵母进行麦芽汁的分批发酵。

Among 558 isolates, 65 （11.6 ~）isolates produced hyperproduction AmpC β-lactamases, 135 （24.2 ~） iso- lates produced ESBLs,and 28（5.0%）produced both enzymes.

对阴沟肠杆菌进行耐药性监测及去阻遏持续高产AmpC酶和ESBLs检测，能较好了解该地区细菌耐药的现状，为当地临床医生抗生素的使用提供合理建议。

In order to explore the mechanisms of auxin action and metabolism, as well as the changing characteristics of its relative enzymes, IAA content in strawberry fruits with different treatments was determined by HPLC. Content of IAA and the activity of IAO and POD in fruits treated by NPA or TIBA, the inhibitor of auxin transport, were analyzed to investigate the changing characteristics and possible source of auxin, as well as the relation among IAA, POD and IAO. Results were showed as the followings: there had a certain count of IAA in receptacles and achenes, and IAA content in achenes was higher than that in receptacles.

摘要为了探索草莓果实中生长素的代谢变化及其发挥作用的机理，利用HPLC定量测定了草莓果实发育过程中瘦果和花托中的吲哚乙酸，结合生长素运输抑制剂NPA和TIBA处理考察了草莓果实中IAA含量以及不同存在状态的生长素氧化酶和过氧化物酶活性变化，综合分析了草莓果实中IAA 的变化特性、可能来源及其与生长素代谢相关酶的关系。

In vitro digestion methods for monogastric animal were reviewed accordi ng to simulatation of sites of the digestive tract,sources of digestive enzymes and the ways for separating digested and indigested nutrients in digesta.

本文从畜禽消化道模拟部位、消化酶的来源和分离已消化养分的方法等几方面对单胃动物体外消化模拟技术进行了归类和总结。

Insecticidal activities of 5-aminolevulinic acid on Oxya chinensis and the effect on activities of three enzymes were studied.

研究5-氨基乙酰丙酸(5-aminolevulinic acid，ALA)对中华稻蝗的杀虫活性及对3种酶活性的影响。

Methods: the present study improved isolation of LDL, undertook one-step of density gradient centrifugation, generate E-LDL by treatment LDL with multiple enzymes, direct fluorescent label CRP and LDL, and investigate whether CRP play as intermedial mediator in atherosclerosis. Experiment consists of 8 groups: control group, N-LDL group, ox-LDL group, E-LDL group, CRP+N-LDL group, CRP+ox-LDL group, CRP+E-LDL group.

本研究参考文献改进LDL提取方法，通过一次性密度梯度超速离心，分离LDL，并且通过复合酶处理以及铜离子氧化法分别生成E-LDL和ox-LDL，并直接对CRP及LDL进行荧光标记，通过与内皮细胞共孵育，利用荧光显微镜检测CRP及LDL与内皮细胞结合，实验分为8组：空白对照组、CRP组、N-LDL组、ox-LDL组、E-LDL组、CRP+N-LDL组、CRP+ox-LDL组、CRP+E-LDL组。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

Objective To research the effects of extract of ginger on testis enzymes of X-ray irradiated mice.

目的：研究生姜提取物对X射线损伤小鼠睾丸酶的作用。

The cell suppression ratio and surviving ratio were detected respectively under conditions of simple hypoxia, or 5mmol/L glucose complicated with hypoxia. The mRNA levels of key enzymes that are involved in regulating glyco-metabolism were detected, including the mRNA levels of pyruvate-kinase, hexokinase, succinic dehydrogenase and isocitric dehydrogenase, and the activity of lactate dehydrognase.

以SMMC-7721人肝癌细胞和L02正常肝细胞作为研究对象，分别在单纯缺氧及加葡萄糖缺氧条件下，观察细胞生长，并检测糖代谢关键酶：丙酮酸激酶(pyruvate-kinase， PK)、己糖激酶(hexokinase， HK)、琥珀酸脱氢酶(succinic dehydrogenase， SDH)、异柠檬酸脱氢酶(isocitric dehydrogenase， IDH) mRNA表达水平和乳酸脱氢酶(lactate dehydrogenase， LDH)活性。

Thee isoenergetic diets containing 0%, 15% and 30% gelatinized corn starch were formulated as control, middle level and high level carbohydrate diets, respectively. The juvenile southern catfish, Silurus meridionalis Chen (50.5±0.6g) were acclimated with control diet for l5d at 27.5±0.5t. Activities of glycolytic key enzymes (HK, GK and PFK-l) and the serum glucose concentrations in the southern catfish respectively fed with the three diets were examined, at 3, 6, 12, 24 and 48h after feeding.

配制含0%、15%和30%糊化玉米淀粉的等能饲料分别作为对照、中水平和高水平碳水化合物饲料，以体重为50.5±0.6g的南方姑幼鱼为实验对象，在水温27.5±0.5℃的条件下以对照饲料驯化15d后，分别测定了以三种饲料投喂的南方鲇在餐后3、6、12、24和48h的己糖激酶、葡萄糖激酶和磷酸果糖激酶(PFK-1)的活性和血糖水平。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。