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The PCR product was cloned into pCR BluntⅡ-TOPO vector to form subclone and then sequenced.The subclone product were cloned into the improved pBluescript Ⅱ KS vector in proper order by suitable enzyme sites to obtain viral full length genomic cDNA clone.

根据测序结果，选择合适的酶切位点将各个亚克隆产物逐段克隆入改造过的pBluescript Ⅱ KS载体中，从而获得了病毒的全长基因组cDNA克隆。

One form of this enzyme makes the prostaglandin that causes pain and swelling.

这种酶的一种形态制造了这种能够产生疼痛和扩张的前列腺素。

Now targeted disruption of the Jhdm2a gene in mice shows that the enzyme is involved in spermatogenesis and regulation of transition nuclear protein and protamine genes.

现在对小鼠Jhdm2a基因的定向破坏表明，这种酶涉及精子生成和转换核蛋白及鱼精蛋白基因的调控。

The objective of this study was to optimize the composition of enzyme protectant of chlorpyifos-biodegradation fungi and determine its stability.

优化毒死蜱高效降解酶液保护剂组成配方，明确其在不同条件下降解毒死蜱的稳定性。

Proteinase inhibitors play an important role in the research of enzyme studies and the structure function relationship of proteins.

蛋白酶抑制剂在酶学及蛋白质的结构与功能关系研究中有重要意义，Kunitz型丝氨酸蛋白酶抑制剂是其中最重要的，也是研究最广泛的蛋白酶抑制剂之一。

These alterations are related to a conformational change of the enzyme resulted from proteolysis.

这些变化与被修饰的酶出现的构象改变有关。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

This paper reporte dthe results of replacement of human Ciq with guinea pig Clq isolated by pseudoglobulin precipition method in solid phase enzyme-linked immuno-sobent assayfor the detection of human circulating immune complexes.

本文首次报道了以优球蛋白沉淀法提取的豚鼠C_1q替代人C_1q，作固相酶联免疫吸附试验检测人循环免疫复合物的结果。

Based on the rabbit-anti-PRV IgG and McAb, the double sandwich enzyme-linked immunosorbent assay for detection of Pseudorabies virus was developed.

利用所制备的兔抗PRV IgG和McAb建立了检测PRV的"单克隆抗体双夹心ELISA"。

Methods 112 blood samples of occupationally lead-exposed workers were selected from a puddling factory in the suburb of Chengdu. An assay based on the polymerase chain reaction-restriction fragment length polymorphism technique of restriction enzyme Bsm Ⅰ was used to detect at the same time.

整群抽取成都市郊区某炼铁厂接铅作业人员112人，抽取其静脉血进行血铅、血清钙和基因多态性测定，按基因型测定结果分组分别比较其血铅、血清钙的差异，并进行血铅和血清钙的相关性分析。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。