查询词典 enzyme

The major route of β-alanine production for pantothenate in Escherichia coli is through the α-decarboxylation of L-aspartate, which is catalysed by the enzyme L-aspartate-α-decarboxylase. The panD mutant of E. coli is β-alanine auxotrophy.

在大肠杆菌中，用于泛酸生物合成的的β-丙氨酸主要是由L-天冬氨酸α位脱羧而产生，这个生化反应由panD基因编码的L-天冬氨酸-α-脱羧酶催化。

The enzyme then removes the phosphate group from that molecule and sticks it onto pantothenic acid.

然后泛酸激酶将后者的磷酸基团移除并把磷酸基团"粘在"泛酸上。

Na〓 inhibited enzyme activity strongly. The kinetic parameters of Km and kcat against methyl parathion at 25℃ was 68. 6±5. 1μmol/L and 45 ±6 S〓 respectively.

Na〓对甲基对硫磷水解酶有较强的抑制作用。25℃时该酶对甲基对硫磷的米氏常数Km为68.6±5.1μmol／L，kcat为45±6 S〓。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Enzyme can cause burning, paresthesia, and dermatitis to surrounding skin.

正确分布软膏可确保效果，酶可致周围皮肤出现烧灼感、感觉异常和皮炎等。

All of them were bled prior to and 3 years after entering this programme and measured the antibody against epidemic parotitis and rubella by enzyme-linked immunosorbant assay.

在疫苗接种前和三年后各抽血一次，然后用 EL ISA方法检测接种前后血清中特异性抗体的浓度，并观察接种组和对照组这两种传染病的发病情况。

The results obtained by the correlation, path and principal component analysis showed that soil urease and alkaline phosphatase activities could be as a comprehensive index of soil fertility, and their enzyme activities are affected by soil chemical properties and other enzymes.

结果表明，土壤脲酶和堿性磷酸酶活性可以作为土壤肥力的指标，且酶活性大小受到土壤化学性质和其它酶活性影响。

The newly constructed nano-gold-baesd gene chip combined with restrictive enzyme digestion without PCR can be widely applied in the simulta- neous detection of Mycoplasma, Chlamydia, fungus, virus and bacteria. It shows a bright prospect in increasing the throughput of identi- fieation of pathogene.

本研究构建的基于限制性酶切非PCR法的纳米金芯片技术可以同时实现对细菌、真菌、支原体、衣原体及病毒等微生物的检测，适用性广且对大幅度提高检测通量有积极意义。

In addition, the restriction enzyme map of activins is high similar between white peafowl and blue peafowl.

氨基酸功能位点分析表明，活化素βA亚基成熟肽可能在细胞信号传递过程中发挥了很重要的作用。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。