查询词典 enzyme

Diketoreductaseis a class of new enzyme, and compared with other oxidoreductase, whose uniqueness lies in its ability to restore at the same time reduced to two hydroxy carbonyl and a high degree of stereoselectivity.

双羰基还原酶（Diketoreductase，简称DKR）是一类全新的酶，与其他的氧化还原酶相比，其独特之处在于它能够同时还原两个羰基还原成羟基，并有高度的立体选择性。

Second, it is successful to try applying the dripping method of suspended cells obtained by enzyme treatment instead of traditional method for pachytene chromosomes preparation.

第二，改变普遍采用的粗线期染色体制备的传统方法，尝试用酶解滴片法制备了水稻减数分裂染色体，获得了成功。

After linearisation by restriction enzyme PacI, the recombinant plasmids were transfected into HEK 293-cells and amplified to give large quantities of infectious recombinant adenovirus carrying the sequences of si-FOXO1s, designated Ad-si-FOXO1-1, Ad-si-FOXO1-2, Ad-si-FOXO1-3, Ad-si-FOXO-pan and Ad-NC, respectively.

人工合成si-FOXO1s的cDNA序列，退火形成的双链cDNA克隆到腺病毒表达载体pAdTrack-CMV上，经PmeI线性化后与pAdEasy-1细胞内同源重组，最后在HEK293细胞中包装为重组腺病毒，分别命名为Ad-si-FOXO1-1、Ad-si-FOXO1-2、Ad-si-FOXO1-3、Ad-si-FOXO-pan和Ad-NC。

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

An enzyme of pancreatic juice that hydrolyzes proteins to form smaller polypeptide units.

这种形式的投资是为你的孩子提供日后经济保障的上好方法。

Soy protein isolate ; soy peptide ; angiotensin converting enzyme inhibitory activity ; in vitro digestion ; pepsin ; pancreatin

大豆分离蛋白；血管紧张素转化酶抑制剂；体外消化；胃蛋白酶；胰蛋白酶

Combizym, an agent of double-deck pellet containing Oryz-Aspergillus enzyme and pancreatin, has been used in patients with dyspepsia for decades in Western countries. However, there is not yet any data of the efficacy and safety of this agent in China.

背景：米曲菌胰酶片是含有米曲菌酶和胰酶的口服双层包膜复合消化酶制剂，在国外用于治疗消化不良已有数十年，然而目前国内尚无米曲菌胰酶片治疗消化不良症状疗效和安全性的资料。

The story is based on a study of the 3-D structure of an enzyme called pantothenate kinase, which triggers the first step in the production coenzyme A, a molecule that is indispensable to all forms of life.

整个故事基于泛酸激酶的三维结构的研究，它触发辅酶A产生过程的第一步，辅酶A对于所有生命来说都是不可缺少的。

The researchers already knew that different types of bacteria build their own versions of the enzyme pantothenate kinase, which are called Types I, II and III.

研究者已知不同类型的细菌有自己特有的泛酸激酶，称为类型I，类型II，类型III。

The pantothenate kinase enzymes consist of two strands of amino acids that fold into various twists and turns to make a complex 3-D structure, White said. These modules, called monomers, snap together to form the enzyme.

每一类型氨基酸都由自己独特的外形和大小，泛酸激酶包含两条氨基酸链，经过折叠扭曲形成复杂的三维结构，这些分子称为单体，彼此咬合形成这个酶。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。