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The flic gene of App was obtained by PCR based on highly homology with Escherichia coli, Salmonella, Shigellosis. Then it was legated to pMD-18T vector. The recombinant plasmid was identified by enzyme and sequence analysis. The 528 bp fragment of flic gene was successfully cloned into pGEX-KG vector, then the recombinant plasmid was transformed into E.

以猪传染性胸膜肺炎放线杆菌血清Ⅰ型菌株基因组为模板，根据其鞭毛区与其它细菌高度同源性设计引物，利用PCR方法扩增鞭毛蛋白基因flic片段，将其亚克隆到pMD-18T载体中并进行PCR和酶切鉴定，再将其亚克隆与载体pGEx-kG分别双酶切和连接，构建重组表达载体pGEX-flic，并将其转化大肠杆菌工程菌BL21，进行原核表达。

Four white-rot fungi, Pleurotus eryngii, Polyporus versicolor, Pleurotus ostreatus and Fomes lignosus, are tested for their abilities to produce lignin peroxidase, manganese peroxidase and laccase in N-limited and N-rich media with shake culture. The four white-rot fungi only produce manganese peroxidase and laccase. Pleurotus eryngii and Pleurotus ostreatus are selected, due to their high enzyme activities, for the degradation and decolorization of alkali lignin.

首先观察了四种白腐菌Pleurotus eryngii，Polyporusversicolor，Pleurotus ostreatus和Fomes lignosus在限氮和富氮培养基中振荡培养产木素过氧化物酶、锰过氧化物酶和漆酶的能力，由于Pleurotus ostreatus和Pleurotus eryngii产酶能力较强，用于碱木素的降解脱色效果较好，二者在限氮条件下11天对碱木素降解脱色率为65%和72%。

This paper discribes character and application of fish enzyme and fish protein in food industry.

综述了鱼酶及鱼蛋白的性质及其在食品工业中的应用。

Four vectors of pET vector system were chosen to express the enzyme in E. coli BL21(DE3). The main results were as follows: 1. The total RNA was obtained from fungus Syncephalastrum racemosum.

以总状共头霉菌株总RNA为模板，通过RT-PCR方法，获得了去除自身信号肽的天冬氨酸类蛋白酶syncephapepsin基因。2。

They were reversible un-competitive inhibitors, and their inhibition constants of free enzymeK(subscript I were 254.00 and 136.78 mmolL^(-1), respectively. Urea was reversible mixed-type inhibitor, and its inhibition constants of free enzymeK(subscript I and enzyme-substrate complexK(subscript IS were determined to be 379.02 and 1182.00 mmolL^(-1), respectively.

Na2SO3和H2O2对酶的抑制均表现为可逆反竞争性类型，它们对游离酶的抑制常数K(下标 I分别为254.00和136.78 mmolL^(-1)；尿素对酶的抑制表现为可逆的混合型抑制，它对游离酶的K和对酶－底物复合物的抑制常数K(下标 IS分别为379.02和1182.00 mmolL^(-1)。

Now, a lot of genes engineering antibodies, enzyme, incretion, plasma proteins, and vaccines have been expressed in the leaves, stems, roots, fructification, and other plant cells and organisms.

现在多种基因工程抗体、酶、激素、血浆蛋白和疫苗等都已在植物的叶、茎、根、果实、种子以及植物细胞和器官中得到表达。

There are two kinds according to reaction mechanism,they are depolymerizing Enzyme and Pectinesterase.Peclinase are generally discovered in fruitage and microorganism. It was widely used to defecation procession of juice in food industry, procession being retted of flax in spinning industry and feedingstuff additive in farming.

按其作用机理分为果胶解聚酶和果胶酯酶两大类；它普遍存在于植物果实和微生物中，广泛应用于食品工业的果汁澄清加工，纺织工业麻类脱胶，畜牧业的饲料添加剂等。

BACKGROUND: The choline acetyltransferase is the key synthetic enzyme for acetylcholine, and is the important symbol of the functional activity of the cholinergic system.

背景：胆碱乙酰化转移酶是乙酰胆碱合成的关键酶，是胆碱能神经系统功能活动的重要标志。

Methods Keratinocytes monolayers and Malassezia furfur were co-cultured in vitro, and the modulating effects of Malassezia furfur on the expression of TLR-2 was measured by immuocytochemistry methods, the expression and secretion of sICAM-1 and IL-8 in keratinocytes was detected by enzyme linked immunosorbent assay.

马拉色菌和角质形成细胞体外共培养，通过免疫细胞化学方法检测角质形成细胞TLR-2蛋白的表达状况，并运用ELISA方法检测马拉色菌对角质形成细胞sICAM-1和IL-8表达和分泌的调节作用。

The relation of dry yeast,proteinase,saccharifying enzyme on the yield of fusel oil in liquor in the solid fermentation was investigated in this paper.

研究了固态发酵白酒中，酵母、糖化酶和蛋白酶的添加与杂醇油生成量的关系。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。