查询词典 enzyme

The result of immobilization showed that hydrophile membranes were more advantageous than hydrophobe ones to act as immobilization carrier. Crosslinking organophosphorus hydrolase by glutaraldehyde on polyethersulfone membrane is a simple and practical immobilization method. An appropriate amount of bovine serum albumin and crosslinker is necessary to get a good result of immobilization. In the biodegradation of methyl-parathion through enzyme membrane reactor, the effect of immobilized enzyme amount, flow rate of peristaltic pump, pH of the feed, and the methyl-parathion concentration on biodegradation rate was studied. It was shown that the biodegradation rate increased with immobilized enzyme amount and substrate concentration. Biodegradation rate didn't increase when the folw rate of the peristaltic pump was larger than 7 ml/min.

然后，研究固定化酶降解甲基对硫磷，从不同材料的膜载体固定有机磷水解酶的比较中，发现亲水膜比疏水膜适合作固定化酶载体；在此基础上，将有机磷水解酶固定在聚醚砜微滤膜上，并制成酶膜生物反应器，用于降解甲基对硫磷；戊二醛化学交联法固定时，酶液与10%牛血清白蛋白溶液的比例在3：1~2：1，与交联剂的比例在7：2~7：4范围内，固定化效果较好，并发现牛血清白蛋白和膜载体有降低酶活损失的作用；将固定化的酶膜装于酶膜反应器降解甲基对硫磷，实验结果表明：反应器的降解速率与固定化酶量呈正比，也随底物浓度的增加而加快，当流速低时，降解速率随流速增大而加快，到7ml/min以上时，降解速率不随流速脑黾佣洌到獾淖畹团ǘ任？

The results indicates that enzyme combination diluent is suited for the production of HCV antibody enzyme linked immunosorbent diagnostic reagents,if the enzyme combination diluent regards PBST as buffer liquid and BSA and Casein irrelated albumen as stability .

结论以PBST为缓冲液，以BSA和C as ein无关蛋白作稳定剂组成酶结合物稀释液适用于HCV抗体酶联免疫诊断试剂的生产。

In this study, we investigate the heat-induced inhibition of drug-metabolizing enzyme activity in mice fed with freeze-dried, heated freeze-dried or half amount autolyzed freeze-dried Brussels sprouts powder. 0.5% ethoxyquin diet was fed to another group of mice as a positive control. The study mice, four per group, were fed a diet consisting of 20% Brussels sprouts for 7 days. Each group had its own pair-fed group. We found significantly greater inductions of glutathione S- transferase, quinine reductase, and phase Ⅱ drug-metabolizing enzyme activities in groups fed within freeze-dried and heated freeze-dried Brussels sprouts than their pair-fed groups. These findings indicate that intestinal microflora still can hydrolyze glucosinolates without plant myrosinase activity, though the alteration of cytochrome- P-450 1A (CYP1A, a phase Ⅰ drug-metabolizing enzyme) induction was only 1.4-fold.

本实验欲进一步探討加热处理之抱子甘蓝对phase Ⅰ与phase Ⅱ解毒酵素活性之影响差异，实验分三组，抱子甘蓝(1)冷冻乾燥组（glucosinolate未裂解）(2)加热冷冻乾燥组(3)半量之裂解冷冻乾燥组（glucosinolate已裂解），(4)and 0.5% ethoxyquin diet was used as a positive control以2%重量混合成饲料，餵以小白鼠（4只／组）7天，结果发现，在第(2)组小白鼠GST phase Ⅱ酵素）活性显著上升，显示在植物裂解酵素不存在之状况下，肠胃道之微生物仍可促进裂解反应，但由CYP1A活性增加不显著（仅1.4倍）看来，根据定义：单独phase Ⅱ酵素活性增加，为monofunctional induction/inducer，可加速致癌物之代谢。

Compared with thermozyme,the first-class structure change of the psychrophilic enzyme can lead to space structure change and the structure of the enzyme is more flexible.Thus,this enzyme can catalyze with less energy in low temperature.

与嗜温酶相比，嗜冷酶一级结构的变化导致空间结构的变化，使酶分子的构象具有更大的柔性，同时也有利于这类酶在低温条件下需要更少的能量而发挥催化作用。

Study on 6 soil enzyme activities and 7 siol fertility factors of 12 different planting modes,analyse the dates with the correlation analysis and the canonical correalation analysis,In order to reveal the relations between the soil enzyme activities and soil fertilities in the low hill lands along Yantze River , The result of the correlation analysis showed that they had a great relation between the soil enzyme activities and siol fertility factors.

对12种不同种植模式的6个土壤酶活性和7个土壤肥力因子进行测定，运用相关分析和典型相关分析方法对数据进行分析，以期阐明沿江丘陵地区土壤酶活性与土壤肥力因子的关系。相关分析的结果表明土壤酶活性与土壤肥力因子密切相关，多数呈极显著正相关。

Studied the stachydrine dealing with the Oncomelania hupensis's esterase、the glycogen and the total protein. The result showed when dealt with the Oncomelania hupensis for 1~2 days, the figures of enzyme are higher than the compared group; when dealt for 3~4 days, the figures of enzyme reached the highest level; when dealt for 5 days, the figures of enzyme remarkably reduced.

用益母草中水苏碱处理钉螺导致其酯酶同工酶、糖原及总蛋白变化的实验结果表明：处理1~2d后钉螺的酶活高于清水对照组，处理3~4d后钉螺的酶活最强，而处理5d后钉螺样品的酶活则大大减弱。

The pH stability of enzyme was 8 and the temperature stability of enzyme was 30℃. Bap-degrading enzyme catalyzed the oxidation of veratryl alcohol to veratraldehyde without H2O2 and the oxidation of Mn2+ by H2O2 to Mn3+.

酵素在将veratryl alcohol氧化生成veratraldehyde时是不需要H2O2协助催化，然而在对Mn2+离子氧化生成Mn3+离子时若有H2O2协助催化下可以加快酵素催化的速度。

Enzyme technology has been used in oil and fats research for years, such as enzyme degumming for oils, enzyme modification for lecithins and biodiesel productions.

酶反应技术在油脂研究中已经得到了一定的应用，如酶法脱胶、磷脂酶法改性、生物燃料的研究等等。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。