查询词典 enzyme

To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin, and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay, the antibody was purified with rProtein A sepharose fast flow and dephosphorylated antigen membrane. The antibody titrate reached 1:10 000 after purification and its special property was enhanced with absorption treatment of dephosphorylated antigen membrane. In addition, we used rabbit anti-PRAS40 antibody and the phosphorylated PRAS40 (Ser183) antibody to detect PRAS40 expression in several cell lines, including the normal cells HL7702, HEK293, tumor cells HepG2, A549 and S180. There were no quite difference among these cells; otherwise, we observed the decreased phosphorylation level of Ser183 after amino acid withdrawal treatment.

为了制备PRAS40(Ser183)磷酸化多克隆抗体，本实验通过蛋白疏水性抗原性分析设计多肽抗原，用其免疫家兔获得抗血清， ELISA检测其效价为1：10 000； Western blotting法检测发现，通过rProtein A Sepharose亲和层析纯化并经非磷酸化的抗原条吸附处理后的抗体可以明显提高磷酸化抗体的特异性；用PRAS40抗体及PRAS40(Ser183)磷酸化抗体对正常细胞HL7702、HEK293及肿瘤细胞HepG2、A549、S180的检测显示：磷酸化的Ser183在不同细胞中表达差异不显著，而在经细胞饥饿处理的HEK293细胞中却明显观察到了S183磷酸化水平随氨基酸含量降低而减弱的现象。

Many factors such as pancreatic enzyme, polymorphonuclear neutrophil, oxygen free radical, cytokine, microcirculatory disturbance, complement, kinin, NO and ET play an important role in the pathogenesis of SAP by interacting with each other.

胰酶、中性粒细胞、氧自由基、细胞因子、微循环障碍、补体系统、激肽、一氧化氮以及内皮素等因素，通过彼此的相互作用和影响，在SAP发病机制中起着十分重要的作用。

ACE is also known as kininase II, part of the kallikrein-kinin- prostaglandin system. As kininase II, this enzyme is involved in the breakdown of kinins, which are potent vasodilators.

ACE也称为激肽酶II，是激肽释放酶-激肽-前列腺素系统的一部分，激肽是一种强有力的血管扩张因子，激肽酶II介导激肽降解。

In order to overcome defect of traditional koumiss, the technology for the koumiss processed from juice mixed with milk peptides hydrolyzed by protein enzyme was studied by orthogonal methods and contrast tests.

为克服传统乳酒缺陷，该文采用蛋白酶水解法及酒精酵母乳酸菌混合发酵法研究了牛乳蛋白多肽复合果汁乳酒的生产工艺。

Objective To analyze the activation mechanism of hepatic apoptotic enzyme Caspase-3 during the early stage of liver regeneration from the perspective of Kupffer cell.

目的 探讨大鼠肝再生早期枯否细胞介导的肝细胞凋亡执行酶Caspase-3的激活机制。

The exemplary anti-cancer compositions of the present invention may also be used as adjuncts with one or more of the following: a surgical procedure, a laser therapy, a micro-radiation pellet implant, a cryogenic procedure, a chemotherapy, a radiation therapy, a electrodessication/curettage, a laetrile therapy, an immuno-therapy, and an enzyme therapy.

本发明技术抗癌制剂独特之处还在于可以与下列之一或多者结合使用：手术治疗、激光疗法、微放射颗粒植入、冷冻疗法、化学疗法、放射疗法、电烧法/刮除术、苦杏仁苷、免疫疗法和酶替代疗法。

Key words 〗 Soil ; Heavy metals ; Microorganisms; Enzyme activity; Lead zinc mining area

关键词〗土壤；重金属；微生物；酶活性，铅锌矿区

The reason may be related to different adsorption mechanism of them under different addition order of ligands and enzyme.

这可能与它们加入顺序不同导致的吸附机理不同有关。

In GENETIC ENGINEERING, the joining of DNA fragments by the action of the enzyme DNA ligase.

在基因工程中，在 DNA 连接酶的作用下连接 DNA 片段的过程。

Describe the molecular action of the enzyme DNA ligase.

请描述 DNA 连接酶的分子作用机制。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。