查询词典 enzyme

The results show that the optimal hydrolysis conditions of these three enzymes were:50℃,pH 8.5, substrate concentration 1:3,enzyme dosage 1200U/g,time 4h for Alcalase; 45℃,pH 8.1, substrate concentration 1:3, enzyme dosage 1200U/g, time 4h for Trypsin;50℃, pH5.5, substrate concentration 1:3, enzyme dosage 1400U/g, time 6h for Bromelin, respectively. The compound enzymes hydrolysis was investigated on the basis of the single enzyme hydrolysis.

利用单因素试验和正交试验分别对这三种酶单独水解牡蛎的条件进行了研究，得出三种酶水解牡蛎的最佳条件分别为Alcalase：温度50℃，pH8.5，料水比1：3，加酶量1200U/g，时间4h；胰蛋白酶：温度45℃，pH8.1，料水比1：3，加酶量1200U/g，时间4h；菠萝蛋白酶：温度50℃，pH5.5，料水比1：3，加酶量1400U/g，时间6h；为牡蛎生物活性肽的开发提供了基础参考数据。

The effects of water content and the added amount of triethylamine on peptide synthesis by immobilized enzyme were different from that of free enzyme. The catalytic efficiency of immobilized enzyme was better than that of free enzyme in most cases. The reuse times of immobilized α-chymotrypsin on MCM-22 are about 4-5 times.

结果表明，有机溶剂中含水量、三乙胺的加入量对自由酶与固载化酶的影响程度不同；多数情况下固载化酶的催化效果优于自由酶；吸附固载化酶的重复使用次数有限。

Compared with the previous method, the improved route can provide directly a spheroid form of immobilized lipase particles of which the size can be controlled by adding non-ionic surfactants during the preparation process so as to increase the recovery rate of enzyme as well as decrease the external diffusional restriction which can diminish the enzyme activity. The improved method can also alter the hydrophobicity and microstructure of the immobilized enzyme matrix by changing the molar ratios of different alkoxyl silanes so as to enhance the enzyme activity by a"surface active effect"and eliminating the internal diffusional restriction.

与前人的制备方法相比，改进的方法可直接制得球形的固定化酶颗粒，而且颗粒的大小可以通过在制备过程中加入非离子型表面活性剂加以控制，从而可以提高固定化过程的酶活回收率和减小外扩散效应对酶的表观催化活力的影响；改进的方法还可以通过改变原料硅氧烷的种类及配比来改变最终制得的固定化载体的疏水性质及微观结构，从而通过对脂肪酶的&界面活化&作用和减小内扩散效应提高固定化脂肪酶的表观酯化活力。

Our purpose is simulating the catalytical action of enzyme in biologic system, so we can directly investigate the factors relating to the functions of enzyme by studying the catalyzing behavior of model molecules imitating enzyme\'s functions. And it is a base of synthesizing high performance model enzyme.

从得到的实验数据我们可以看出：锌是酯水解反应的活性中心；并且金属中心相同时，配位数相同的配合物，结构越简单，其催化酯水解效果越好；含有配位不饱和金属中心的锌配合物表现出更好的催化效果；配合物中咪唑基团的存在可有效的增强这个配合物催化酯水解的效果。

By the partial purification of Arthrobacter nicotinovorus D-97 endocellular enzymes and the results of enzymatic reaction of enzymes of partition collection, it was found that enzymes related to trehalose synthesis were consisted of Trehalose-forming Enzyme and Trehalose-releasing Enzyme . Trehalose-forming Enzyme could convert maltooligosaccharides into non-reducing maltooligosyl trehalose by intramolecular transglycosylation, and Trehalose-releasing Enzyme could hydrolyze α-1, 4 linkage between the trehalose residue and the maltooligosaccharide residue to release trehalose and maltooligosaccharides with two glucose monomer lesser.

将食尼古丁节杆菌D-97胞内酶部分纯化并对各收集组分进行酶反应，发现其海藻糖合成酶系由海藻糖生成酶(Trehalose-forming Enzyme，TFE)和海藻糖释放酶(Trehalose-releasingEnzyme，TRE)组成，前者分子内转糖基将直链麦芽寡糖转化为没有还原性的麦芽寡糖基海藻糖，后者专一切割麦芽寡糖基海藻糖分子上麦芽寡糖基与海藻糖连接的α-1，4糖苷键，从而释放出海藻糖和减少了两个葡萄糖单体的麦芽寡糖。

Phenylmethylsulfonyl fluoride (174.2 mg/mL), chicken ovomucoid (1000 mg/mL) and soy-bean trypsin inhibitor (1000 mg/mL) could inhibit enzyme activity, which indicated that the enzyme belonged to serine protease group. On plasminogen-free fibrin plates and plasminogen fibrin plates, the fibrinolytic activity had no obvious difference, indicating that the enzyme was a fibrinolytic enzyme which degraded fibrin directly, but not a plasminogen activator which degraded fibrin by activating plasminogen.

此菌株产生的纤溶酶在50℃以下和pH5.0~11.0范围内具有较好的稳定性，最适作用温度为42℃；最适pH值为9.0；Mg2+、Ca2+对此酶有明显的激活作用，而Cu2+能完全抑制酶的活性；174.2 mg/mL的苯甲基磺酰氟、1000 mg/mL的鸡卵类粘蛋白和1000 mg/mL大豆胰蛋白酶抑制剂能完全抑制酶活性，初步说明此酶属于丝氨酸蛋白酶类；体外溶纤作用表明，该酶溶解纤维蛋白的方式是直接溶解，而不是通过激活纤溶酶原。

As an important enzyme in both glycolysis and glyconeogenesis, D-Glyceraldehyde-3-phosphate dehydrogenase has been shown to be an allosteric enzyme, The yeast enzyme binds 〓 with positive cooperativity, whereas the muscle enzyme binds 〓 with negative cooperativity.

D—甘油醛—3—磷酸脱氢酶是生物体内葡糖酵解与糖原异生过程中的关键酶，不同种属的酶在协同效应与半位反应方面表现出较大差异。

The myocardial enzyme, liver duty enzyme, kidney duty enzyme, amylopsin and blood sugar in serum were detected, the results showed that CK, CK-MB, ALT and AMY had different extent changing characteristic, but CRE and BUN no obviously change.

分别于接种病毒后15天和30天剖杀猕猴进行病理学组织学观察，其病变以病毒性心肌炎、间质性肾炎、神经系统的非化脓性炎症及肝脏汇管区有细胞增生性反应为主要特征，其他组织器官也有不同程度的病理变化。

Now the research on HPL focus on dicotyledon, and the research on HPL of monocotyledon still havent been reported. In this paper, the research on cloning of rice HPL and enzyme activity assay were carried out and several result were obtained.1. Enzyme activity assay of rice HPLBased on the method of measure HPL Enzyme activity in other plants, the means on preparation of hydroperoxides and measurement rice HPL activity were set up. The 9-HPL arid 13-HPL activity of several rice varieties with different storability and different dormancy were tested.

本试验建立了水稻的HPL酶活性测定体系，测定了水稻萌发过程中的HPL酶活性，并进行了水稻HPL基因的克隆及表达研究，结果如下： 1、水稻HPL的酶活性测定在参考其它植物HPL酶活性测定方法的基础上，建立了水稻的HPL底物发生体系和酶活性测定体系，并测定了不同贮藏性水稻品种萌发过程中的种胚9-HPL、13-HPL活性和不同休眠性水稻品种种子的9-HPL、13-HPL酶活性。

This article focus on enzyme gene clone and heterogenesis expression,site directed modify and orthogenesis,fu-sion protein and fusion enzyme,enzyme mimics(abzyme and molecular imprinting)and telomere .

本文着重介绍了酶基因克隆与异源表达、酶分子的定向改造和进化、融合蛋白与融合酶、酶的人工模拟和端粒酶，综述了分子酶工程的研究进展、趋势及其应用。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48