查询词典 enzyme

The thesis studied the total exocellular proteins, reductant sugar and the pectinase enzyme activity of CXJZ95-198 strain in glucose, mannose, xylan, mannosan and pectin culture media.Compared enzyme activity of pectinase, xylanose and β-mannanose. The secreted proteins in ferment fluid from different culture media were analysed by SDS-PAGE electrophoresis.

本论文对CXJZ95-198菌株在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中胞外酶蛋白质总量、还原糖及果胶酶活性的影响进行了研究；对果胶酶、木聚糖酶及β-甘露聚糖酶在甘露糖培养基中的酶活进行了比较；对CXJZ95-198菌株在不同碳源培养基中的发酵液在SDS-PAGE电泳中分泌出的蛋白带作了比较；对CXJZ95-198菌株所产生的胞外酶系中果胶酶进行了分离纯化。

The oxidase system includes both membrane-bound and soluble cytosolic proteins. The primary feature of this enzyme is stimulus-dependent activation and which mechanism is the phosphorylation and translocation of a complex of cytosolic factors to the membrane, where they associate with a flavocytochrome enzyme.The fully assembled complex functions as an electron transport system,moving electrons from cytosolic NADPH to molecular oxygen to form superoxide anion（O2-）, which, along with subsequent reactive products called respiratory burst,exerts microbicidal and cytotoxic activities.

此氧化酶体系包括膜结合的和可溶的胞浆蛋白，其主要特征为依赖刺激而活化，机制为复合体的胞浆因子磷酸化并转位至膜，在这里与细胞色素酶相关联，组装完全的复合体在功能上作为一个电子转运体系，从胞浆NADPH转移电子给分子氧形成超氧阴离子（O2-），伴随着接下来的活性物质产生，也叫呼吸爆发，发挥抗菌和抗细胞毒素的活性。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速，应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等，其中自杀基因被认为是最有前景的基因治疗方法，它又称病毒介导的酶／药物前体疗法，是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中，该基因表达的产物可将无毒的药物前体转化为毒性药物，从而选择性杀伤该肿瘤细胞，常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因，前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物，然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷，作为DNA合成链的终止剂和DNA合成酶的抑制剂，干扰细胞DNA的合成；后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶（5-FC）脱氨成为5-氟尿嘧啶（5-FU），然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸（5-FUTP）和5-氟-2′脱氧尿嘧啶-5′磷酸（5-FdUTP），5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成，5-FdUTP则作用于胸苷合成酶，导致TMP衰竭而阻止DNA的合成，最终诱导肿瘤细胞凋亡。

The best combination of isolating protoplast of Mozzie buster was the enzyme mixture containing 2.0% Cellulase R-10, 0.4% Pectinase, 0.5M mannitol as osmoticpressure, 27℃under dark 80r/m rotating enzymolysis 8h, and the highest yieldachieved to 1.67×10~6per gram, the protoplast vitality was 87.4%; The bestcombination of isolating protoplast of Ocimum basilicum was the enzyme mixturecontaining 1.6% Cellulase R-10, 0.4.% Pectinase, 0.5M mannitol as osmotic pressure,27℃under dark 80r/m rotating enzymolysis 8h, and the highest yield achieved to1.67×10~6 per gram, the protoplast vitality was 87.4%.

本研究对酶解最佳条件进行研究，得出分离驱蚊草原生质体酶解条件以CPW-13进行质壁分离1h，2.0％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压，27℃黑暗下80r／m酶解处理8h为好，产量可达1.67×10~6个／g，原生质体活力为87.4％；分离罗勒原生质体酶解条件以CPW-13进行质壁分离1h，1.6％纤维素酶和0.4％果胶酶，0.5M甘露醇为渗透压为好，27℃黑暗下80r／m酶解处理8h，产量可达2.14×10~6个／g，原生质体活力为67.8％。

Purified GO had a molecular weight between 145, 000 and 162, 300, as determined by Sephadex G-200 filtration and SDS-PAGE of the enzyme as cross-linked with glutaraldehyde. This result showed that the holoenzyme was a tetramer, and no change occurred in molecular weight of the enzyme during the s〓orage. The active form of GO from brassica leaves showed a constant molecular weight during purification, so did that from O. sativa leaves during greening of etiolated seedlings, maybe acting as a stable tetramer.

用Sephadex G-200胶过滤和戊二醛交联后的SDS-PAGE法测得纯化的菜心叶片GO的分子量在145，000-162，300间，为四聚体；并且在放置过程中其分子量和聚合态均保持不变，为稳定的四聚体；进一步研究表明菜心叶片GO在纯化过程中和水稻叶片GO在黄化苗的转绿过程中，两种GO活性状态的分子量和聚合态也没有发生变化，均呈四聚体。

According to some deficiencies in using pectinase only, a new kind of enzyme, macerating enzyme , used to process fruit and vegetable is putting forward.

综述了我国果蔬加工的现状，根据目前加工过程中单一使用果胶酶还存在不足的情况，提出了一种新型的果蔬加工用酶——粥化酶。

When the embryo reached the prehatching stage, the hatching enzyme granules were secreted into the perivitelline space from the top of HGCs. Secretory granules were spherical and 0.5-1.0 μm in diameter. The hatching enzyme was dissolved in the perivitelline fluid, where it gained access to the egg envelope and digested the inner layer of the egg envelope.

至孵化前期时，孵化酶颗粒自HGCs顶部的开口分泌出来，分泌颗粒呈圆球形，直径为0.5-1.0 μm，有的以单体的形式存在，有的粘结成团。

The biological tests indicate that most of the compounds synthesized have herbicidal activity in a certain degree and several compounds show certain bactericidal activity. Further anti-ALS enzyme test shows that two kinds of novel phosphonic ureas have good anti-ALS enzyme activity.

对合成的部分化合物进行了初步的生物活性测定，所设计的大部分化合物都显示出一定的除草活性，有的具有一定的杀菌活性；对两类膦酰脲化合物进行了离体ALS酶抑制试验，结果表明它们是很好的ALS酶抑制剂，同磺酰脲相当，有希望作为另一类ALS酶抑制剂的先导化合物。

Finally, it was preliminarily to make use of compound enzyme in the collagen enzyme-degrading reaction.

最后，在对单酶的研究基础上，初步探讨了复合酶对骨胶原的酶解反应。

Psychrophilic enzyme, cold active enzyme...

在理论研究中的重要性

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48