查询词典 enzyme

The relation between accessibility of amino acids and the property of enzyme was discussed, and the relation between accessibility of amino acids and the isoelectronic point of enzyme was studied.

酶总是在一个特定的pH范围内发挥功能。酸碱性质是它的一个重要的理化性质，等电点是它的一个重要的参数，研究它有利于更好的认识酶的功能。

Studies on the enzyme indicated that it was not a Michaelis-Menten enzyme, its kinetical curve was S model, its optim pH is 7. 5, optim tempreture is 19℃ and it should be conserved in and light-avoiding light-salt solution.

以此为材料进行其性质研究，结果表明，该酶为非Michaelis-Menten酶，其动力学曲线呈S型，最适pH为7.5，最适温度19℃，易于在避光、高盐溶液内贮藏。

Lignin-degrading enzyme can be used to delignify directly, among them laccase was believed the most promise enzyme.

直接脱木素是通过木素降解酶来进行的，到目前为止，最有希望的木素降解酶是漆酶。

The dynamics simulation of modified enzyme was carried out in the presence of solvent. The realtionship between structure and activity was analyzed. It was considered the entropy effect which was brought by the side chain of lauric acid patched to the enzme had the main role of increasing the stability of modified enzyme.

并在溶剂存在的条件下对修饰酶进行了分子动力学模拟和构象分析，讨论了其结构与性能的关系，认为月桂酸修饰链无序性带来的效应是提高修饰酶稳定性的主要原因。

By imitating different concentration of Cd2+ Cu2+ Cr3+ Cr6+and Zn2+polluted water environment,we mainly studied their toxic effects on aquatic piants by means of measurement of physiologicai indexes and electron microscopy observation as well as the relieving to Cu2+ injury of antioxidase enzyme system in Lemna minor L. and Vallisneria asiatica Miki in this paper by throwing Ce.the experimental results showed that Cd2+ Cu2+ Cr3+ Cr6+ and Zn2+ might injure photosynthetic systems, protective enzyme system, substance metabolism systems and cell ultrastructure of the plant.

本文通过模拟不同浓度梯度的重金属Cd~(2+)、Cu~(2+)、Cr~(3+)、Cr~(6+)和Zn~(2+)污染环境，利用生理生化测定、扫描电镜、透射电镜、气相色谱及激光共聚焦等实验手段，研究了四种金属离子对不同生态型的水生植物的毒害影响，初步探索了Ce对浮萍Cu害的缓解效应和La、Zn及其交互作用对浮萍活性氧清除系统影响。

The molecular weight of dextransucrase measured by SDS-PAGE was 170 kDa ,which was similar to the enzyme from Leuconostoc mesenteroides. The enzyme had an optimum temperature between 25and 30℃ and an optimum pH of 5.4. It was relatively stable in the range of pH 5.0 to 7.0, but the stability declined rapidly as soon as the temperature rose over 35℃.

以蔗糖为底物，酶促反应的最适温度为25～30℃，最适pH值为5.4，动力学常数Km值为10.43 mmol/L；酶活在pH 5.0～8.0较为稳定，在室温（25℃）保藏4 天仍有59%的酶活力，4℃保存7周酶活力仅下降一半，但在35℃以上失活很快；Ca2+对催化作用有较大的促进，Mg2+有微弱的促进作用，K+对催化反应无影响，Cu2+的抑制作用最强。

These ferments are made of the roofs, leaves and stems of 58 handpicked kinds of plant and vegetables through exclusive the biochemical technology, with the high-reactivity, high-stability and high-safety; The second is the fruit compound ferments(the approval No.J11000510836) with the abundant vitamin, minerals, favorable viable groups, multi-amino acid groups, oligosaccha groups and glucose , is made from handpicked 103 kinds of fruits and 20 kinds of Chinese herbal medicines through microbial treatment and fermentation method; The third is grain and bean high-fiber nutritious ferments (the approval No.J11000600369) with abundant proteases, amyloclast enzyme and lipolytic enzyme, is made from 60 kinds of grains and beans through special biologic technology, and is used to lose weight, expel toxin, clear the bowels, supply nutrition and activate cells in human body.

公司精选台湾58种植物、蔬菜的根、茎、叶天然原料，以独特的生化科技手段研制而成，不仅具有高活性、稳定性和安全性的特点，而且具有耐高温、耐强酸的特点，不含任何添加剂，其活性是当今世界任何厂家生产的酵素都不可比拟的。第二宝是水果复合酵素（检验审核号是J11000510836）。公司精选台湾103种水果，通过微生物科技处理，经半年多时间发酵酿制而成，含有丰富的维生素、矿物质、有益活菌群、氨基酸、果寡糖、葡萄糖等。第三宝是谷豆高纤营养酵素（检验审核号是J11000600369）。公司精选60种谷、豆及种子，用生物科技手段，经过特殊研制而成，含有丰富的蛋白质分解酵素、淀粉分解酵素及脂肪分解酵素。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

Chitosan alginate loaden enzyme have prominent effect for activity of immobilized enzyme by orthogonal experimental design, and getting optimum condition .

经正交实验L_8(2~7)，壳聚糖、海藻酸钠、载酶量对固定化酶活力影响比较显著，确定最佳固定化酶条件。

Therewas a significant characteristic of the vertical distribution of soil enzyme activity,the relationship analysis indicated that there was a significant positive relationshipbetween the catalase activity and OM,and total N,and effective P、K,and ex-changible Mg,there was a negative relationship between the catalase activity andBase Situatiou percentage,and Total Contents of Base;the activity of the polyphe-nol oxidase was positively related with OM,and negatively related with total N andexchangible Ca clearly;The urease activity decreased with CPR increasing,and thechange of the former two kinds of soil enzyme actirity was not significantly withCPR increasing,It is possible to be related with maned disturbance frequently.

随着代次增加，有机质和交换性Ca下降明显；土壤的CEC和交换性酸分别下降了8.8—16.1%和6.8—18.5%，因此，其养分总量亦逐代降低，其中有机质下降幅度最大；土壤酶的活性具有明显的垂直分布特征，相关分析表明，过氧化氢酶活性与土壤OM、全N、有效P、K和交换性Mg有极显著的正相关，而与盐基总量、盐基饱和度呈明显的负相关。多酚氧化酶活性与OM呈显著负相关，与全N、交换性Ca有显著相关。脲酶活性则有随连栽代次增加而有下降趋势，前两种酶在代次之间变化不明显，可能与频繁的土壤干扰有关。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48