查询词典 enzyme-linked immunosorbent assay

The activity of glutamate-pyruvate transaminase andglutamic oxalacetic transaminase was measured using AU5400 automaticbiochemical analyzer; the activity of the hyaluronic acid,the laminin,precollagen typeⅢ type, collagen typeⅣ was evaluated according toradioimmunoassay method; the activity of transforming growth factor beta-1(TGFβ1)and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) was measured according to enzyme linked immunosorbent assay. Results First, the effect of Baoganning on CCL_4 induced hepatic fibrosis in rats.

研究结果一、保肝宁对四氯化碳(carbon tetrachloride CCL_4)复合因素致大鼠肝纤维化模型的作用： 1、与正常组动物血清相比，模型组动物血清中的ALT、AST、HA、LN、PCⅢ、CⅣ、TGFβ1、TIMP-1显著升高(P＜0.05)；与模型组相比，保肝宁高、中、低剂量组血清中的ALT、AST、HA、LN、PCⅢ、CⅣ、TGFβ1、TIMP-1显著降低(P＜0.05)；秋水仙碱组、复方鳖甲软肝组动物血清中上述指标呈不同程度的下降趋势。

Root,immunoassay of ELISA(enzyme linked immunosorbent assay) was employed to determine IAA concentration in root tip, unilateral application of agar block containing IAA on root tip was carried out to investigate the effect of exogenous IAA on phototropic response.

为了研究生长素在水稻根负向光性反应中的作用，用含有IAA的琼脂块贴附在种子根的根尖表面观测IAA对根的生长效应，用ELISA法测定根尖的IAA含量，并对种子根弯曲部分进行半薄切片，结果表明：(1)种子根的生长方向既受光的调控也受外施的IAA的调控，根尖向贴有含IAA琼脂块的一侧弯曲生长；(2 )不定根在光照 1.5h后，背光侧的IAA含量明显大于向光侧；(3)种子根尖发生弯曲生长是伸长区的细胞不均等生长所致。

Methods After taking triazolam and distilled water respectively, the mice sleeping-behavior state was observed and recorded; the ratio of sleeping mice was recorded after taking different of ingredients Eucommia ulmoides Oliv. combining with pentobarbital sodium at subliminal dose, and mice latent period and sleeping time at supraliminal dose. Measure the content of interleukin-1β(IL-1β) in mice brain with enzyme-linked immunosorbent assay, weigh spleen and thymus of mice.

通过观察记录日间小鼠睡眠行为状态，戊巴比妥钠阈下催眠剂量实验，延长戊巴比妥钠睡眠时间实验，酶联免疫吸附反应分别检测服用3种不同极性的提取部位、阳性对照物和空白对照组的小鼠脑组织中IL-1β含量，并结合脏器指数等综合分析。

Seven inactivated tetravalent vaccines of Riemerella anatipestifer,of which three (1#,3#,7#) employed oil-emulsion,two(4#,6#) employed propolis,two(8#,10#) employed aluminum hydroxide as their basic adjuvants,were developed and compared by the methods as follows:challenges were performed by homologous virulent RA strains to measure their protective abilities;indirect enzyme-linked immunosorbent assay was used to monitor antibody production;lymphocyte transformation test was performed to study the expression of cell-mediated immunity.

本实验通过对供试鸭的攻毒保护试验、通过用ELISA方法对供试鸭特异性抗体消长规律的研究以及通过用~3H-TdR掺入法对供试鸭T细胞免疫水平的检测三个方面对所研制的七种不同佐剂类型的鸭疫里氏杆菌四价灭活苗的免疫原性进行了比较研究。

Methods: Blood samples were taken from 40 RRTI children (26 males and 14 females, mean age of 3.68±1.47 years) and 35 normal children. Enzyme linked immunosorbent assay method was used to detect the levels of serum tIgE and Western blotting method was used to detect positive percentage of sIgE.

RRTI患儿40例，对照组健康儿童35例，采用酶联免疫吸附法检测血清tIgE水平，欧蒙印迹法检测血清吸入与食物组抗原sIgE抗体阳性率。

Methods Three serologic methods including domestic-made enzyme-linked immunosorbent assay reagents, the toluidine red unheated-serum test and treponema pallidum particle agglutination test reagents were compared. The transudate fluid of the syphilis patient in genital duct was detected by polymerase chain reaction for analysis of infectivity.

采用国产酶联免疫吸附试验试剂与甲苯胺红不加热血清试验和梅毒螺旋体颗粒凝集试验进行血清学方法比较，采用聚合酶链反应对梅毒患者生殖道皮损渗出液进行检测，分析其传染性。

On the basis of purification and immunity of vitellin, antibodies of vitellin were obtained and used to measure the concentration of vitellin in embryo in different stages of embryonic development by enzyme-linked immunosorbent assay.

在采用纯化的卵黄磷蛋白免疫兔子而获得其抗体的基础上，运用酶联免疫吸附检测法(enZyme一linked ilnlnunosorbent assay，ELISA)分析测定了发育过程中胚胎内卵黄磷蛋白的含量。

Methods: Renal IRI models were made in rats(the right kidney was resected, and the left renal artery was closured by a clip for 1 hour and then was loosened to reperfusion). The concentration of TNF-a, IL-8 and IL-6 were detected by double antibody sandwich enzyme-linked immunosorbent assay. Using biochemical analyzer, concentration of serum urea nitrogen and creatinine were detected.

采用大鼠肾I-R模型(将大鼠右肾切除，夹闭左肾蒂1h再，松开使左肾再灌注)，用双抗体夹心ELISA法动态检测肾组织和血液中TNF-a、IL-8、IL-6含量，以及用生化分析仪测定SUN、Scr等指标变化。

Enzyme-linked immunosorbent assay and coagulating time method were used to assess antigenic stability and coagulating stability,respectively.

以酶联免疫吸附法测定了蛇毒降纤酶的抗原活性稳定性，并与凝固时间法测定的生物活性稳定性进行了比较。

To export whether the ovariectomy induced increase in bone resorbing activity present in supernatants fraction of bone marrow and the effects of endogeneous IL-6 on bone resorption, the bone resorbing activity present in marrow supernatants and the neutralizing effects of antibody against IL-6 were examined by the release of 〓Ca from perlabeled fetal rats parietal bone, the concentration of IL-6 were measured by Enzyme-Linked Immunosorbent Assay .

为了探讨卵巢切除是否导致骨髓上清骨吸收活性增加，以及白细胞介素-6在骨吸收中的作用，通过胎鼠颅骨〓释放实验测试骨髓上清的骨吸收活性及IL-6抗体的中和作用，通过ELISA测骨髓上清IL-6浓度。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48