查询词典 enzyme-linked immunosorbent assay

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件，最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1：25倍稀释、最佳反应时间为2h；与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应；阳性样品经64倍稀释后依然可检出，检测灵敏度是传统微孔板ELISA的4倍。

ACA was detected in the sera of 176 patients and 98 members in control group by enzyme linked immunosorbent assay.

用酶联免疫吸附试验对176例急性脑血管病患者和98例健康对照者进行ACA检测。

Syphilis serodiagnosis/methods; Enzyme-linked immunosorbent assay; Antibodies, bacterial

梅毒血清诊断／方法酶联免疫吸附测定抗体，细菌

METHODS: A series of N-octyl-N'-succinyl chitosan labeled with fluorescein, were incubated with HepG2 liver cancer cells, K562 leukemia cells, A549 human lung cancer cells, and BGC cancer cells respectively and followed by using flow cytometry and enzyme-linked immunosorbent assay to assess the affinity and inhibition of N-octyl-N'-succinyl chitosan for the four kinds of tumor cells already mentioned.

用异硫氰酸荧光素对N-正辛基－N'-琥珀酰基壳聚糖进行标记，再分别与人肝癌细胞(HepG2)、人白血病细胞(K562)、人非小细胞肺癌细胞(A549)及人胃癌细胞共培养，通过流式细胞仪及酶联免疫检测仪测定N-正辛基－N'-琥珀酰基壳聚糖对肿瘤细胞的亲和性及抑制力。

An indirect competitive enzyme-linked immunosorbent assay was established for detecting vitellogenin in the whole body homogenate of male swordtail fish.This technique was developed with antiserum of lipovitellinresistance as antibody and Vtg as antigen.

以卵黄脂磷蛋白(lipovitellin，Lv)抗血清为抗体，以纯化的卵黄蛋白原(vitellogenin，Vtg)为抗原，建立了间接酶联免疫吸附反应方法检测剑尾鱼雄鱼整体匀浆液中ρ。

The enzyme-linked immunosorbent assay was used for the content measurement of myelin basic protein in telencephalon tissue at 1 week, 1 month, 3 months and 6 months after irradiation.

SD大鼠受2、10或30Gy全脑照射后的1周以及1、3和6个月时，采用酶联免疫吸附法测量脑组织中髓鞘碱性蛋白含量、用Luxol fast blue染色法和电镜观察并分析髓鞘的病理学改变。

Methods: Serum sICAM-1, IL-15 and monocyte ADCC of 66 Spleen-Deficiency patients were determined by enzyme-linked immunosorbent assay and modified thiazole blue test.

采用酶联免疫吸附法和改良噻唑蓝法对66例脾虚患者血清sICAM-1和白细胞介素-15(IL-15)及单核细胞抗体依赖性细胞介导的细胞毒反应进行检测。

Methods SYBR Green I real-time polymerase chain reaction was used to detect the expression of VEGF mRNA in articular cartilage from 20 OA patients and 10 controls with traumatism. VEGF protein was detected by enzyme-linked immunosorbent assay in articular cartilage.

应用SYBR Green Ⅰ实时定量聚合酶链反应检测20例OA和10例外伤患者关节软骨中VEGF mRNA表达，并用酶联免疫吸附试验测定关节软骨组织VEGF含量。

Objective: To analyze the enzyme-linked immunosorbent assay from clinical samples to detect Treponema pallidum antibody positive results in the false-positive and response measures.

目的：分析酶联免疫吸附试验从临床样本中检测出梅毒阳性结果中的假阳性及应对措施。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48