查询词典 enzyme-linked immunosorbent assay

Up-regulated proteins under salt stress include an ABC transporter permease, glycerol-3-phosphate permease, pyrimidine nucleotide transporter, formate dehydrogenase, and down-regulated proteins include succinate dehydrogenase iron-sulfur subunit, flavoprotein subunit, cytochrome b-556 subunit, and a hypothetical membrane protein similar to charperone DnaJ. Some of these changes were further verified by enzyme activity assay.

盐胁迫诱导上调表达的蛋白包括ABC型转运蛋白，3-磷酸甘油透性酶，嘧啶核苷转运蛋白和甲酸脱氢酶；下调表达的蛋白包括琥珀酸脱氢酶铁硫亚基、黄素蛋白亚基、细胞色素b556亚基，以及分子伴侣DnaJ的同源蛋白，酶活力测定结果表明胁迫条件下上述蛋白的活性变化与表达量变化相一致。

All results from morphological, physiological and biochemical characterization, the 16S rDNA sequence and phylogenetic assay identified that the strain belongs to Micrococcus luteus. The DNA fragment encoding mature peptide of fibrinolytic enzyme was cloned by PCR and sequenced.

利用传统细菌分类鉴定方法对其形态和生理生化特征进行了研究，发现其与藤黄微球菌的特征相符， 16S rDNA序列分析的结果也表明它与藤黄微球菌的16S rDNA序列的同源性高达99%，因此该菌在系统分类学上属于微球菌属藤黄微球菌。

Methods 112 blood samples of occupationally lead-exposed workers were selected from a puddling factory in the suburb of Chengdu. An assay based on the polymerase chain reaction-restriction fragment length polymorphism technique of restriction enzyme Bsm Ⅰ was used to detect at the same time.

整群抽取成都市郊区某炼铁厂接铅作业人员112人，抽取其静脉血进行血铅、血清钙和基因多态性测定，按基因型测定结果分组分别比较其血铅、血清钙的差异，并进行血铅和血清钙的相关性分析。

The influence of copper ion on enzyme activity of hog liver copper zinc superoxide dismutase was monitored by using the pyrogallol autoxidation inhibition assay.

利用邻苯三酚自氧化法监测在磷酸盐缓冲体系中Cu2＋对猪肝铜锌超氧化物歧化酶活力的影响，认为Cu2＋与猪肝CuZnSOD存在直接相互作用。

The result of enzyme assay revealed that polygalacturonaseand polygalacturonate lyase play key role in the retting process.

酶活性测定显示PGase和PAL在构皮浸解过程中起关键作用。

Objective To develop a assay for testing the concentration of thyroxine in human serum by Enzyme immunoassay.

目的 以T4 (3，5 ，3' ，5' -四碘-L -甲状腺素)测定为模式，研究建立血清激素固相试管酶免测定方法。

Through Enzyme assay of biochemical to detect the inhibitory effect of the extract on the activity of tyrosinase.

动物细胞实验检测提取液对小鼠B16黑素瘤细胞增殖抑制率的影响，细胞内黑色素含量和细胞内酪氨酸酶活力测定。

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

Because of no signal from N-terminal protein sequencing for 43kDa protein directly, a resulting 18kDa peptide from the partial cleavage of 43kDa protein by BrCN had its N-terminal sequences of AFTFKK determined by N-terminal protein sequencing assay. Then, the 43kDa protein was convinced to the enzyme of 3phosphate glycerate kinase (PGK1) by searching YPD.

由于43kDa蛋白直接进行蛋白质氨基端序列测定时没有信号，因而用溴化氰部分化学裂解43kDa蛋白，得到的18 kDa多肽经蛋白质氨基端序列测定，得到其氨基端前6个氨基酸残基序列为AFTFKK，通过计算机检索YPD，确定43 kDa蛋白是3—磷酸甘油酸激酶（PGK1）。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。