查询词典 enzyme-linked immunosorbent assay

The purified protein was detected by chicken chorioallantoic membrane angiogenesis inhibitory assay and endothelial cell proliferation inhibitory assay.

将纯化的蛋白质通过鸡胚尿囊膜血管生成抑制实验和内皮细胞抑制实验对纯化的蛋白质进行生物学活性测定。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

The inhibitory effects of ATRA and its nickel complexes on K562 cell lines have been investigated by using MTT colorimetric assay and clonic assay.

采用MTT比色法，克隆形成法研究了该配合物及全反式维甲酸对K562细胞增殖的影响。

Methods: Growth inhibition of dl1520 on CNE-2 cells was examined with MTT colorimetric assay and cytopathic effect assay.

用MTT法和细胞病变试验测量d11520在体外对CNE-2细胞的抑制和杀伤作用。

The confirmative tests of weak-reactive samples included neutralization assay for specific antigen and immune-blotting or immuno-fluorescence antibody assay for specific antibody.

确认方法可分为特异抗原确认的中和试验和特异抗体确认的免疫印迹或荧光抗体试验。

GdCl3(10mg/kg) or the same volume of NS was continually injected of vein at 48h and 24h before LPS(5mg/kg) was injected in the male kunming species mouse. Then took out of liver or isolated KCs 30 minute after LPS was injected and assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of liver or KCs in vivo.Ⅱ. Isolated and cultured KCs of mouse, 1 h pretreatment with GdCl3; Culture medium with LPS(100ng/ml) was added and continuatively incubated 30 minute. Assay the protein expression and phosphorylation level of ERK1/2 and p38MAPK of KCs in vitro and detection effect of GdCl3 on its phagocytosis function in respectively.

Ⅰ 雄性昆明种小鼠在注射LPS（5mg/kg）前48h、24h，分别静脉注射GdCl3（10mg/kg ）或等量的生理盐水，于LPS或生理盐水注射后30min，分别取出肝脏或分离KCs，检测肝脏或KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平；Ⅱ体外培养小鼠KCs经GdCl3（100μM）预处理1h，加入含LPS（100ng/ml）的DMEM培养基继续孵育30min，分别检测体外培养KCs ERK1/2、p38MAPK蛋白表达及磷酸化水平和GdCl3对其吞噬、分泌功能的影响。

Methods Electrophoretic mobility shift assay and transient transfection assay were used in this study.

采用电泳迁移改变分析和荧光素酶报告基因瞬时转染分析。

Genesee strives to establish its core product pipelines in antibodies and recombinant antigens to meet the needs of a wide variety of diagnostic techniques, such as latex agglutination assay, capture ELISA, double antigen ELISA, double antigen-mediated lateral flow immuno-chromatograph, chemoluminescence, multiplex microsphere assay.

公司致力于抗体和重组蛋白两种核心原材料产品的开发，以满足各种诊断技术的需求，如乳胶凝集试验法，间接法ELISA，双抗原ELISA，双抗原介导的胶体金快速诊断，化学发光法，蛋白液体晶片多重诊断技术。

Trypan blue exclusion assay and cell growth curve were used to observe proliferation. Fluctuation of CD11b, reactive oxygen species and NF-кB were detected using flow cytometry. Cell apoptosis was observed using DNA laddering assay.

选择NB4细胞，加入ATRA和不同剂量的As2O3，观察细胞生长曲线，流式细胞仪检测CD11b、ROS、NF-кB的变化，DNA片段梯度观察细胞凋亡。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样，经微球菌溶解实验证明，3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L；从每组小鼠的12种组织提取总RNA，经Dot blotting检测证明，三种重组载体除在乳腺中表达外，p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达；在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。