查询词典 enzyme-linked immunosorbent assay

In cells assay, the TATI could promote the pseudopodium and anchorage-independent colony formation, but the tumorigenesis assay in animal should be further clarification.

在研究TATI蛋白质在细胞阶层中的作用时，发现TATI蛋白质会影响细胞侵犯能力及非贴附性生长能力，但其在动物体内的所扮演角色需进一步验证。

This interaction was verified by GST pull-down assay and further confirmed by co-localization assay in SH-SY5Y cells.

在上述工作的基础上，我们深入研究了BRI〓与SCG10相互作用的生物学效应。

The assay is based on TaqMan 3-minor groove binder chemistry using the outer membrane protein B gene as the target. The assay amplified R. rickettsii but other members of the genus rickettsia did not produce positive reactions, nor did other members of the order rickettsiales or any non-rickettsial bacterium.

建立的定量标准曲线Ct值与模板拷贝数呈良好线性关系，最低检测浓度为5拷贝/μl，与普通PCR相比较，敏感性是普通PCR的100倍；用该方法检测其它相关立克次体和常见非立克次体病原菌，检出结果均为阴性。

Nuclear Run-on Assay was used to address the contribution of transcriptioninitiation to the regulation of five histones by YSV. Actinomycin D inhibitor studies were used to detect the mRNA half-life of five histones. SLBP weremeasured by Western blot assay to identify regulation of YSV on Pre-mRNAsplicing.

应用Nuclear Run-on Assay观察YSV对人肝癌BEL-7402细胞的五种组蛋白转录效率的影响；应用放线菌素D转录抑制法观察YSV对组蛋白mRNA半衰期的影响；应用Western blot检测SLBP的表达水平观察YSV对组蛋白mRNA加工的影响。

And Homo Sapien fetal brain cDNA Library was used to screen PRL-3-interacting proteins. 3. Identification and bioinformatical analysis of CDH22, a candidate PRL-3-interacting protein Interaction between PRL-3 and CDH22, a candidate PRL-3- interacting protein was identified by GST-pull down assay, co-immunoprecipitation assay and co-localization analysis in vivo and in vitro.

PRL-3相互作用蛋白的鉴定及其功能的生物信息学分析运用基于蛋白质水平的谷胱甘肽-S-转移酶融合蛋白沉淀、免疫共沉淀和共定位分析等技术对酵母双杂交系统初步筛选的结果进行体内和体外的验证。

Objective: To investigate the effects of sulindac on the growth and motility of ovarian cancer cell SKOV3 and its involved mechanism. Methods: In order to detect the effects of sulindac on cultured SKOV3, sulindac with different concentration and untreated control were set up. The changes in morphology of SKOV3 were observed by phase contrast microscopy. MTT colorimetric assay was used to study the effects of sulindac on SKOV3 cell growth. Motility of SKOV3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌SKOV3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（MMP2）、环氧合酶-2(COX-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对SKOV3细胞形态的影响，采用MTT法观察舒林酸对SKOV3细胞生长的影响，通过划痕试验观察舒林酸对SKOV3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对SKOV3细胞MMP2、COX-2表达的影响。

Methods The effects of various concentrations of tea polyphenols on proliferation, invasion and apoptosis of CGTH W-3 cells were observed by MTF assay, plate scarification assay and flow cytometer, respectively.

方法用MTT法检测细胞增殖活性，观察不同浓度茶多酚对CGTH W-3细胞增殖活性的影响；并通过培养板划痕法观察茶多酚对CGTH W-3细胞侵袭能力的影响；应用流式细胞仪检测各浓度茶多酚诱导CGTH W-3细胞凋亡的作用。

Methods: in order to detect the effects of sulindac on cultured skov3, sulindac with different concentration and untreated control were set up. the changes in morphology of skov3 were observed by phase contrast microscopy. mtt colorimetric assay was used to study the effects of sulindac on skov3 cell growth. motility of skov3 was determined by scarification assay.

目的：研究舒林酸对卵巢癌skov3细胞的生长和细胞运动的影响以及对基质金属蛋白酶2（mmp2）、环氧合酶-2(cox-2)表达的影响：方法：将不同浓度的舒林酸加入培养液中观察其对skov3细胞形态的影响，采用mtt法观察舒林酸对skov3细胞生长的影响，通过划痕试验观察舒林酸对skov3细胞运动的影响，利用免疫细胞化学方法研究舒林酸对skov3细胞mmp2、cox-2表达的影响。

ABSTRACT A large passive-active neutron shuffler for the assay of drum containing uranium and transuranic waste is a nondestructive assay instrument which can work in passive and active mode.

大型桶装铀及超铀废物分类装置是一种非破坏性分析仪器，可工作于无源和有源两种方式。

Before repellency and anti-silverfish assay, the culture system for silverfish was established in this study. The best feeds for silverfish were cellulose and mixed feed in a 1:1 ration (the composition of mixed feed was milk powder: oatmeal: yeast= 1: 9: 9). Moreover, the hatching process was not necessary for the mature of silverfish eggs. According to the results obtained in the repellency assay, the essential oil of leaves and woods from C.

为了进行忌避和致死试验，本研究首先对衣鱼进行饲养及孵化条件测试，於实验后得知最适合衣鱼生长之条件为纸张纤维与混合饲料(酵母粉：麦皮：高蛋白＝9：9：1)混合比例为1：1时之生长状况最佳；另外，衣鱼卵并不需要成虫的孵化，而虫卵的生活条件则与成虫一致。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。