查询词典 enzyme-linked immunosorbent assay

Two modified beacons containing of uracil and hypoxanthine have been used to validate the assay on purified proteins with success. However, while applying the assay to cell extracts, the results were interfered by non-specific nuclease activities.

我们分别设计其中含有尿嘧啶以及inosine的分子信号，以测定尿嘧啶-核酸醣解酶（uracil-DNA glycosylase）以及次黄嘌呤-核酸醣解酶（hypoxanthine-DNA glycosylase）的活性。

MethodsOne point assay and two point assay were used to detect 20 respective samples of normal,hemolysis and icterus,lipaemia.

方法分别采用一点终点法和两点终点法检测正常标本、溶血或黄疸标本、脂血标本各20例。

METHODS Plasma endotoxin of patient and healthy people were detected by turbidimetric assay and gel-clot assay with limulus reagents.

分别用定量动态比浊法和鲎试剂定性凝胶法检测革兰阴性菌感染患者和正常健康对照血浆内毒素含量。

In this study, semi-quantitative RT-PCR, ABC immunohistochemistry assay, flow cytometry analysis and luciferase/luciferin assay were used to study the expression and function of CD39 in seven hematopoietic cell lines.

采用半定量RT-PCR、ABC免疫酶标和流式细胞术以及荧光素/荧光素酶法，研究了七个血源细胞系中CD39的表达和功能。

In the second part, in order to compare the effect of function of different SNPs, we use functional assay to detect the LUM under the condition of site-directed mutation. Moreover, since MAP kinase pathway is important in survival and apoptosis of cells, we use luciferase reporter assay to detect the influences of the transcription of LUM in mitogen-activated protein kinase pathway.

本研究的第二部分在探讨这些基因多型性的功能，这是以表现型功能性质体的作用，藉由各种不同基因多型性在其中的表现，来比较这些不同的基因变异是否会有功能上的改变，及比较其造成改变的强弱。

Nonradioactive spectrophotometric HAT assay is an alternative method to the widespread radioactive assay but suffers from drawbacks as lack of sensitivity and accuracy.

非放射性分光光度HAT测定方法虽然可替代广泛使用的放射性检测方法，但缺乏灵敏度和准确性。

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

A sandwich ELISA was established taking the antiserum as the primary antibody. The sensitivity of the ELISA was 0.412 ng GH/ml of sample. Inter-assay and intro-assay coefficients of variation were 2. 58％(n=6) and 5. 69％(n=6) respectively. Serial dilutions of serum and pituitary homogenate from orange-spotted grouper yielded dose response curves that were parallel to the standard curve. And the GH of Acanthopagrus butcheri was also parallel to the standard curve. Prolactin of goldfish and recombinant growth hormone of common carp showed no cross-reactivity with the antiserum of orange-spotted grouper GH. It showed that the established RIA was specific and sensitive.

以斜带石斑鱼重组GH抗血清为第一抗体，羊抗兔抗体为第二抗体建立了双抗体夹心酶联免疫测定法，该系统的灵敏度为0.412ng GH／ml(n=6)，组内变异系数为2.58％(n=6)，组间变异系数为5.69％(n=6)，斜带石斑鱼血清和脑垂体稀释液曲线与斜带石斑鱼重组GH蛋白的标准曲线相平行、黑棘鲷GH的系列稀释曲线与标准曲线相平行，金鱼催乳素、重组鲤鱼GH的系列稀释曲线与标准曲线不平行，表明建立的斜带石斑鱼GH ELISA具有良好的特异性和重复性，灵敏度达到了测试血清样品中GH的水平。

Virus removal efficiency is determined by the real time PCR and inactivation efficiency is determined by cell-based infectivity assay.Enveloped DNA virus removal and inactivation: Real time PCR indicated that method based on gold magnetic particles coupled with antiserum has shown great superiority on virus removal. Cell-based infectivity assay indicate pasteurism has shown superiority on virus inactivation.

对两种指示病毒分别进行巴氏灭毒和偶联抗血清金磁颗粒处理，实时荧光定量PCR检测核酸变化确定病毒的去除效率，同时进行病毒的细胞感染力实验确定病毒的灭活效率，结果显示：在对DNA有包膜病毒的去除能力上，金磁颗粒法明显优于普通的巴氏灭活法；在对DNA有包膜病毒灭活能力上，巴氏法明显优于金磁颗粒法。

Methods: Human leukemia cell line K562 was treated with different concentrations of WM. The proliferation of K562 cells was examined by MTT assay. DNA damage in K562 cells was examined by single cell gel electrophoresis assay, and apoptosis of K562 cells was detected by Annexin V-FITC/PI double staining. The expressions of total Akt, phosphorate Akt, and NF-κB p65 mRNA and protein were detected by RT-PCR and Western blotting, respectively.

以不同浓度的渥曼青霉素作用于人类髓细胞白血病细胞K562，采用MTT法检测细胞增殖活性，单细胞凝胶电泳技术检测细胞DNA损伤形成的"彗星"样拖尾现象，Annexin V FITC/PI双标法检测细胞凋亡，Western blotting、RT-PCR检测渥曼青霉素作用K562细胞后总Akt和磷酸化Akt以及NF-κB基因及蛋白表达水平的变化。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。