查询词典 enzyme-linked immunosorbent assay

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RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

Water electroplating factory: an area of over 1,000 square meters, has eight professional and technical personnel, employees 80 people; have metal, plastic plating production lines linked to the one, linked to production of various chromium, linked to nickel, bronze, bronze, gold, pearls Lak And so on.

水电镀厂：占地1000多平方米，拥有专业技术人员8人，员工80人；拥有五金、塑挂镀生产线各一条，生产各样挂铬、挂镍、古铜、青铜、金、珍珠叻等。

Just started a three-day fever, the highest 39.1 degrees, was later linked to the hospital for three days of water, the fourth day of diarrhea and vomiting since the afternoon, go to the hospital linked to water, hanging by nearly a week见好not actually check the stool also found fungal infection, immediately after the cessation of linked water, to do massage with the enema, it is not diarrhea, but is still very hard stools, a one, may I ask how can we rule fungal infection?

宝宝前阵子拉肚子，挂了好几天的水，抗生素用多了，霉菌感染怎么办？刚开始三天发烧，最高39.1度，后来去医院挂了三天的水，第四天下午开始腹泻呕吐，去医院挂水，挂了近一个礼拜都不见好，居然还检查大便后发现霉菌感染，后马上停止挂水，改做推拿跟灌肠，现不腹泻，但大便还是很硬，一粒一粒，请问怎么才能治霉菌感染？

Isolation, cultivation and identification of human embryonic NSC from cerebral cortex of 7-12 week abortus. By using flow cytometry and MTT assay, the effects of various concentration of TSPG and TSPG cooperating with cytokines in NSC culture media for 3 days on proliferation of human embryonic NSC has studied. By employing immunocytochemistry assay of the expression of tyrosine hydroxylase, the effect of different dilution of TSPG and TSPG cooperating with IL-1 on induced differentiation of human embryonic NSC into dopaminergic neuron has researched.

从7～12周人工流产胚胎脑组织中分离培养人胚胎NSC，免疫细胞化学鉴定NSC特异性nestin抗原表达，在NSC的培养体系中加入TSPG，采用流式细胞术和MTT法检测不同浓度TSPG以及TSPG与EGF，bFGF联合应用对人胚胎NSC增殖的影响；经免疫细胞化学检测培养细胞酪氨酸羟化酶表达，分析不同浓度TSPG以及TSPG与IL-1联合应用对NSC定向诱导为DA能神经元的影响。

To study the changes of plasma endotoxin NO and hemorheology in experimental cirrhotic rats and their correlation,we have used hemorheology analysis system and the means of limulus amoebocyte lystata assay Griess assay to detect changes of plasma endotoxin NO and hemorheological parameter in rats at 3 6 9 weeks after CCl4 administration.

为探讨肝硬化中血浆内毒素、NO水平的变化及血液流变学特性的改变及其相互关系，我们通过用四氯化碳(carbon tetrachloride，CCl_4)来诱导大鼠肝硬化模型，采用鲎试剂动态浊度法、镉还原比色法、血液流变学分析仪，分别检测了大鼠肝硬化演变过程中早期(诱导第3周末)、中期(诱导第6周末)、晚期(诱导第9周末)的血浆内毒素、NO水平及血液流变学各参数。

Keywords Sheep;Goat;Anthelmintic resistance;PCR-SSCP;PCR-RFLP;Faecal egg count reduction test;Egg hatch assay;Larval development assay

绵羊； 山羊；驱虫药抗药性； PCR-SSCP； PCR-RFLP；粪便虫卵减少试验；虫卵孵化试验；幼虫发育试验

Upon the optimized Agrobacterum-mediated transformation systems mentioned above, the herbicide-resistant gene bar has proved to be successfully incorporated into genomes of the Cynodon dactylon after PCR assay of hpt gene and bar gene, GUS histochemical assay and hpt resistance evaluation.

经潮霉素抗性基因hpt和除草剂抗性基因bar的PCR分析、GUS组织化学染色和潮霉素抗性鉴定，证实除草剂抗性基因bar已成功整合到狗牙根基因组。

The testing method contained fibrin plate assay, direct and indirect chromogenic assay, SDS-PAGE fibrin autography, and visual exhibition of the complexes between rPAI-1 and t-PA or low molecular weight u-PA on SDSPAGE.

所使用的方法包括：纤维蛋白平板法，直接和间接的显色底物法，SDS-PAGE纤维蛋白自显影法，以及用SDS-PAGE直接显示rPAI-1与t-PA和低分子量u-PA形成的复合物。

The Thiobarbituric acid-reactive substances assay is a sensitivity HPLC assay that measures a chromogen that is produced by the reaction of thiobarbituric acid with malondialdehyde, which is an endproduct of lipid peroxidation.

硫巴比妥酸反应物分析，利用HPLC的灵敏特性分析硫巴比妥酸与丙二醛反应所产生的发色体，其为内生性脂质过氧化物。

You can snipe the second and third union leaders from this position.

您可以鹬第二和第三工会领袖从这一立场出发。

Aiming at the currently shortage of XML streams quality detecting, this paper proposes a new forecasting method of XML streams quality by least squares support vector machines, which is used the method of XML keys' vector matrix as windows, and vector product wavelet transform to multilevel decompose and refactor the XML streams series, that can fulfill real-time checking demand of XML quality, and ensure constraint, consist- ency and integrality. For even more adapting net load, it proposes a control strategy by weight and adaptive adjustment to ensure XML streams quality.

针对当前XML数据流质量检测存在的不足，提出构建XML键的矢量矩阵作为窗口，利用矢量积小波变换多级分解与重构XML数据流，再结合最小二乘支持向量机对XML数据流质量进行预测的一种方法，满足XML数据流质量重构时实时检测的要求，保证XML数据的约束性、一致性与完整性；为了更好的适应网络负载，采取加权与自适应窗口调整等调度策略充分保证XML数据流的质量检测。